Strain Fitness in Shewanella oneidensis MR-1 around SO1301

Experiment: ShewMM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemL and pyrB are separated by 26 nucleotidespyrB and SO1302 are separated by 218 nucleotides SO1300: hemL - glutamate-1-semialdehyde-2,1-aminomutase (NCBI ptt file), at 1,352,002 to 1,353,294 hemL SO1301: pyrB - aspartate carbamoyltransferase (NCBI ptt file), at 1,353,321 to 1,354,340 pyrB SO1302: SO1302 - chloride channel (NCBI ptt file), at 1,354,559 to 1,356,283 SO1302 Position (kb) 1353 1354 1355Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 1353.380 kb on + strandat 1353.407 kb on + strandat 1353.510 kb on - strand, within pyrBat 1353.533 kb on - strand, within pyrBat 1353.543 kb on - strand, within pyrBat 1353.673 kb on + strand, within pyrBat 1353.767 kb on + strand, within pyrBat 1353.775 kb on - strand, within pyrBat 1353.775 kb on - strand, within pyrBat 1353.885 kb on + strand, within pyrBat 1353.998 kb on - strand, within pyrBat 1354.012 kb on + strand, within pyrBat 1354.098 kb on + strand, within pyrBat 1354.113 kb on - strand, within pyrBat 1354.137 kb on + strand, within pyrBat 1354.183 kb on + strand, within pyrBat 1354.227 kb on - strand, within pyrBat 1354.240 kb on + strandat 1354.246 kb on - strandat 1354.248 kb on - strandat 1354.332 kb on - strandat 1354.402 kb on - strandat 1354.447 kb on - strandat 1354.457 kb on - strandat 1354.460 kb on + strandat 1354.461 kb on + strandat 1354.516 kb on - strandat 1354.537 kb on + strandat 1354.540 kb on - strandat 1354.545 kb on - strandat 1354.545 kb on - strandat 1354.550 kb on - strandat 1354.551 kb on - strandat 1354.557 kb on + strandat 1354.626 kb on + strandat 1354.667 kb on + strandat 1354.672 kb on + strandat 1354.672 kb on + strandat 1354.677 kb on + strandat 1354.680 kb on - strandat 1354.680 kb on - strandat 1354.680 kb on - strandat 1354.685 kb on - strandat 1354.717 kb on + strandat 1354.747 kb on + strand, within SO1302at 1354.753 kb on - strand, within SO1302at 1354.767 kb on + strand, within SO1302at 1354.770 kb on - strand, within SO1302at 1354.810 kb on - strand, within SO1302at 1354.844 kb on - strand, within SO1302at 1354.885 kb on - strand, within SO1302at 1354.943 kb on + strand, within SO1302at 1355.172 kb on - strand, within SO1302at 1355.182 kb on - strand, within SO1302at 1355.182 kb on - strand, within SO1302at 1355.189 kb on + strand, within SO1302at 1355.189 kb on + strand, within SO1302at 1355.203 kb on - strand, within SO1302at 1355.308 kb on - strand, within SO1302

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Per-strain Table

Position Strand Gene LocusTag Fraction ShewMM
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1,353,380 + -2.2
1,353,407 + -1.7
1,353,510 - pyrB SO1301 0.19 -3.5
1,353,533 - pyrB SO1301 0.21 -3.4
1,353,543 - pyrB SO1301 0.22 -4.3
1,353,673 + pyrB SO1301 0.35 -1.4
1,353,767 + pyrB SO1301 0.44 -3.0
1,353,775 - pyrB SO1301 0.45 -4.2
1,353,775 - pyrB SO1301 0.45 -2.3
1,353,885 + pyrB SO1301 0.55 -2.9
1,353,998 - pyrB SO1301 0.66 -1.2
1,354,012 + pyrB SO1301 0.68 -4.6
1,354,098 + pyrB SO1301 0.76 -4.8
1,354,113 - pyrB SO1301 0.78 -0.8
1,354,137 + pyrB SO1301 0.80 -2.0
1,354,183 + pyrB SO1301 0.85 -1.4
1,354,227 - pyrB SO1301 0.89 -4.7
1,354,240 + -3.1
1,354,246 - -4.2
1,354,248 - -2.9
1,354,332 - -2.4
1,354,402 - +0.8
1,354,447 - -0.4
1,354,457 - -0.4
1,354,460 + -0.2
1,354,461 + +0.4
1,354,516 - -0.2
1,354,537 + -0.3
1,354,540 - +1.2
1,354,545 - +1.3
1,354,545 - +0.6
1,354,550 - -0.7
1,354,551 - -0.5
1,354,557 + +0.6
1,354,626 + +0.0
1,354,667 + +3.3
1,354,672 + +0.2
1,354,672 + -0.9
1,354,677 + -0.4
1,354,680 - -0.2
1,354,680 - +0.4
1,354,680 - -0.6
1,354,685 - -0.4
1,354,717 + -0.3
1,354,747 + SO1302 0.11 -0.6
1,354,753 - SO1302 0.11 +0.2
1,354,767 + SO1302 0.12 +0.2
1,354,770 - SO1302 0.12 -0.6
1,354,810 - SO1302 0.15 -0.1
1,354,844 - SO1302 0.17 -0.2
1,354,885 - SO1302 0.19 -0.7
1,354,943 + SO1302 0.22 -0.7
1,355,172 - SO1302 0.36 +0.3
1,355,182 - SO1302 0.36 +0.3
1,355,182 - SO1302 0.36 +0.2
1,355,189 + SO1302 0.37 +0.4
1,355,189 + SO1302 0.37 -1.2
1,355,203 - SO1302 0.37 -0.8
1,355,308 - SO1302 0.43 -0.3

Or see this region's nucleotide sequence