Experiment: ShewMM_MES_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4305 and xerC overlap by 1 nucleotides xerC and SO4307 are separated by 41 nucleotides SO4307 and dapF are separated by 11 nucleotides dapF and lysA are separated by 70 nucleotides
SO4305: SO4305 - HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (NCBI ptt file), at 4,482,896 to 4,483,618
SO4305
SO4306: xerC - integrase/recombinase XerC (NCBI ptt file), at 4,483,618 to 4,484,517
xerC
SO4307: SO4307 - conserved hypothetical protein (NCBI ptt file), at 4,484,559 to 4,485,179
SO4307
SO4308: dapF - diaminopimelate epimerase (NCBI ptt file), at 4,485,191 to 4,486,018
dapF
SO4309: lysA - diaminopimelate decarboxylase (NCBI ptt file), at 4,486,089 to 4,487,333
lysA
Position (kb)
4484
4485
4486 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 4483.568 kb on - strand at 4483.600 kb on + strand at 4483.657 kb on + strand at 4483.666 kb on - strand at 4483.825 kb on + strand, within xerC at 4483.882 kb on - strand, within xerC at 4483.931 kb on - strand, within xerC at 4483.997 kb on + strand, within xerC at 4484.005 kb on - strand, within xerC at 4484.070 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.319 kb on + strand, within xerC at 4484.469 kb on + strand at 4484.500 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.547 kb on + strand at 4484.581 kb on - strand at 4484.699 kb on + strand, within SO4307 at 4484.847 kb on + strand, within SO4307 at 4484.871 kb on + strand, within SO4307 at 4484.880 kb on - strand, within SO4307 at 4484.923 kb on - strand, within SO4307 at 4484.923 kb on - strand, within SO4307 at 4484.923 kb on - strand, within SO4307 at 4484.927 kb on + strand, within SO4307 at 4485.010 kb on - strand, within SO4307 at 4485.100 kb on - strand, within SO4307 at 4485.152 kb on + strand at 4485.172 kb on - strand at 4486.101 kb on + strand at 4486.111 kb on + strand at 4486.119 kb on - strand at 4486.147 kb on + strand at 4486.147 kb on + strand at 4486.147 kb on + strand at 4486.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM_MES_buffer remove 4,483,568 - +0.9 4,483,600 + +0.6 4,483,657 + -2.9 4,483,666 - -1.6 4,483,825 + xerC SO4306 0.23 -0.4 4,483,882 - xerC SO4306 0.29 -0.2 4,483,931 - xerC SO4306 0.35 -1.3 4,483,997 + xerC SO4306 0.42 -1.1 4,484,005 - xerC SO4306 0.43 -1.2 4,484,070 + xerC SO4306 0.50 -2.3 4,484,195 + xerC SO4306 0.64 -1.0 4,484,195 + xerC SO4306 0.64 -0.9 4,484,195 + xerC SO4306 0.64 -0.6 4,484,319 + xerC SO4306 0.78 -1.6 4,484,469 + -1.1 4,484,500 - -3.4 4,484,521 - -0.2 4,484,521 - +0.9 4,484,521 - -0.3 4,484,521 - -0.0 4,484,547 + +0.4 4,484,581 - -0.5 4,484,699 + SO4307 0.23 -0.5 4,484,847 + SO4307 0.46 -1.0 4,484,871 + SO4307 0.50 -1.7 4,484,880 - SO4307 0.52 -1.2 4,484,923 - SO4307 0.59 -2.8 4,484,923 - SO4307 0.59 +0.3 4,484,923 - SO4307 0.59 -0.7 4,484,927 + SO4307 0.59 -1.4 4,485,010 - SO4307 0.73 -0.2 4,485,100 - SO4307 0.87 -2.5 4,485,152 + +0.4 4,485,172 - +0.0 4,486,101 + -4.1 4,486,111 + -3.2 4,486,119 - -3.9 4,486,147 + -6.9 4,486,147 + -4.4 4,486,147 + -5.9 4,486,155 - -6.2
Or see this region's nucleotide sequence