Experiment: ShewMM_MES_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisH are separated by 104 nucleotides hisH and hisB are separated by 144 nucleotides hisB and hisC are separated by 82 nucleotides
SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305
hisA
SO2070: hisH - amidotransferase HisH (NCBI ptt file), at 2,166,410 to 2,167,075
hisH
SO2071: hisB - imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase (NCBI ptt file), at 2,167,220 to 2,168,311
hisB
SO2072: hisC - histidinol-phosphate aminotransferase (NCBI ptt file), at 2,168,394 to 2,169,569
hisC
Position (kb)
2167
2168
2169 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2166.241 kb on + strand at 2166.311 kb on - strand at 2166.483 kb on + strand, within hisH at 2166.483 kb on + strand, within hisH at 2166.510 kb on + strand, within hisH at 2166.552 kb on - strand, within hisH at 2166.575 kb on - strand, within hisH at 2166.656 kb on + strand, within hisH at 2166.792 kb on + strand, within hisH at 2166.942 kb on + strand, within hisH at 2166.943 kb on + strand, within hisH at 2167.012 kb on + strand at 2167.130 kb on - strand at 2167.185 kb on + strand at 2167.193 kb on - strand at 2167.193 kb on - strand at 2167.225 kb on - strand at 2167.294 kb on - strand at 2167.305 kb on + strand at 2167.358 kb on + strand, within hisB at 2167.488 kb on + strand, within hisB at 2167.596 kb on - strand, within hisB at 2167.637 kb on - strand, within hisB at 2167.877 kb on - strand, within hisB at 2167.918 kb on + strand, within hisB at 2168.250 kb on + strand at 2168.423 kb on - strand at 2168.434 kb on + strand at 2168.442 kb on - strand at 2168.442 kb on - strand at 2168.485 kb on - strand at 2168.485 kb on - strand at 2168.737 kb on + strand, within hisC at 2168.737 kb on + strand, within hisC at 2168.777 kb on + strand, within hisC at 2168.883 kb on + strand, within hisC at 2168.891 kb on - strand, within hisC at 2168.913 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2169.078 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.106 kb on - strand, within hisC at 2169.106 kb on - strand, within hisC at 2169.148 kb on - strand, within hisC at 2169.263 kb on + strand, within hisC
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM_MES_buffer remove 2,166,241 + -1.9 2,166,311 - -3.0 2,166,483 + hisH SO2070 0.11 -2.9 2,166,483 + hisH SO2070 0.11 -3.9 2,166,510 + hisH SO2070 0.15 -4.7 2,166,552 - hisH SO2070 0.21 -4.2 2,166,575 - hisH SO2070 0.25 -3.8 2,166,656 + hisH SO2070 0.37 -5.9 2,166,792 + hisH SO2070 0.57 -3.9 2,166,942 + hisH SO2070 0.80 -4.5 2,166,943 + hisH SO2070 0.80 -5.2 2,167,012 + -5.2 2,167,130 - -0.9 2,167,185 + -5.0 2,167,193 - -0.8 2,167,193 - -0.7 2,167,225 - +0.3 2,167,294 - -1.9 2,167,305 + -4.3 2,167,358 + hisB SO2071 0.13 -4.6 2,167,488 + hisB SO2071 0.25 -4.0 2,167,596 - hisB SO2071 0.34 -3.6 2,167,637 - hisB SO2071 0.38 -5.3 2,167,877 - hisB SO2071 0.60 -3.1 2,167,918 + hisB SO2071 0.64 -3.1 2,168,250 + -5.3 2,168,423 - -3.8 2,168,434 + -3.3 2,168,442 - -3.2 2,168,442 - -3.1 2,168,485 - -1.7 2,168,485 - -4.2 2,168,737 + hisC SO2072 0.29 -2.5 2,168,737 + hisC SO2072 0.29 -3.2 2,168,777 + hisC SO2072 0.33 -3.9 2,168,883 + hisC SO2072 0.42 -3.8 2,168,891 - hisC SO2072 0.42 -4.8 2,168,913 - hisC SO2072 0.44 -4.8 2,168,951 - hisC SO2072 0.47 -3.0 2,168,951 - hisC SO2072 0.47 -3.8 2,168,951 - hisC SO2072 0.47 -4.3 2,169,078 + hisC SO2072 0.58 -2.3 2,169,098 + hisC SO2072 0.60 -0.1 2,169,098 + hisC SO2072 0.60 -1.4 2,169,098 + hisC SO2072 0.60 -3.3 2,169,098 + hisC SO2072 0.60 -2.4 2,169,098 + hisC SO2072 0.60 -2.7 2,169,098 + hisC SO2072 0.60 -3.1 2,169,098 + hisC SO2072 0.60 -0.7 2,169,106 - hisC SO2072 0.61 -2.1 2,169,106 - hisC SO2072 0.61 -2.8 2,169,148 - hisC SO2072 0.64 -2.8 2,169,263 + hisC SO2072 0.74 -4.5
Or see this region's nucleotide sequence