Strain Fitness in Shewanella oneidensis MR-1 around SO2068

Experiment: ShewMM_MES_buffer

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisI and hisF are separated by 82 nucleotideshisF and hisA overlap by 19 nucleotideshisA and hisH are separated by 104 nucleotides SO2067: hisI - phosphoribosyl-ATP pyrophosphatase/phosphoribosyl-AMP cyclohydrolase (NCBI ptt file), at 2,164,095 to 2,164,730 hisI SO2068: hisF - hisF protein (cyclase) (NCBI ptt file), at 2,164,813 to 2,165,586 hisF SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305 hisA SO2070: hisH - amidotransferase HisH (NCBI ptt file), at 2,166,410 to 2,167,075 hisH Position (kb) 2164 2165 2166Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2163.876 kb on - strandat 2164.152 kb on + strandat 2164.153 kb on - strandat 2164.207 kb on + strand, within hisIat 2164.318 kb on + strand, within hisIat 2164.511 kb on + strand, within hisIat 2164.600 kb on + strand, within hisIat 2164.800 kb on + strandat 2164.800 kb on + strandat 2164.851 kb on + strandat 2164.917 kb on - strand, within hisFat 2164.924 kb on + strand, within hisFat 2164.953 kb on + strand, within hisFat 2165.133 kb on - strand, within hisFat 2165.306 kb on - strand, within hisFat 2165.328 kb on - strand, within hisFat 2165.390 kb on + strand, within hisFat 2165.398 kb on - strand, within hisFat 2165.421 kb on - strand, within hisFat 2165.528 kb on + strandat 2165.529 kb on - strandat 2165.561 kb on - strandat 2165.583 kb on + strandat 2165.602 kb on + strandat 2165.660 kb on + strand, within hisAat 2165.695 kb on - strand, within hisAat 2165.746 kb on - strand, within hisAat 2165.799 kb on - strand, within hisAat 2165.924 kb on + strand, within hisAat 2166.092 kb on + strand, within hisAat 2166.092 kb on + strand, within hisAat 2166.100 kb on - strand, within hisAat 2166.100 kb on - strand, within hisAat 2166.216 kb on + strand, within hisAat 2166.241 kb on + strandat 2166.311 kb on - strandat 2166.483 kb on + strand, within hisHat 2166.483 kb on + strand, within hisHat 2166.510 kb on + strand, within hisHat 2166.552 kb on - strand, within hisHat 2166.575 kb on - strand, within hisH

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Per-strain Table

Position Strand Gene LocusTag Fraction ShewMM_MES_buffer
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2,163,876 - -1.0
2,164,152 + -3.1
2,164,153 - -0.1
2,164,207 + hisI SO2067 0.18 -2.7
2,164,318 + hisI SO2067 0.35 -3.0
2,164,511 + hisI SO2067 0.65 -3.2
2,164,600 + hisI SO2067 0.79 -4.0
2,164,800 + -4.0
2,164,800 + -2.4
2,164,851 + -5.3
2,164,917 - hisF SO2068 0.13 -6.3
2,164,924 + hisF SO2068 0.14 -5.6
2,164,953 + hisF SO2068 0.18 -1.1
2,165,133 - hisF SO2068 0.41 -3.5
2,165,306 - hisF SO2068 0.64 -4.4
2,165,328 - hisF SO2068 0.67 -4.4
2,165,390 + hisF SO2068 0.75 -5.0
2,165,398 - hisF SO2068 0.76 -2.3
2,165,421 - hisF SO2068 0.79 -1.7
2,165,528 + -6.1
2,165,529 - -3.9
2,165,561 - -2.4
2,165,583 + -2.7
2,165,602 + -2.8
2,165,660 + hisA SO2069 0.12 -2.5
2,165,695 - hisA SO2069 0.17 -1.7
2,165,746 - hisA SO2069 0.24 -2.9
2,165,799 - hisA SO2069 0.31 -2.5
2,165,924 + hisA SO2069 0.48 -3.7
2,166,092 + hisA SO2069 0.71 -4.7
2,166,092 + hisA SO2069 0.71 -2.8
2,166,100 - hisA SO2069 0.72 -4.8
2,166,100 - hisA SO2069 0.72 -4.1
2,166,216 + hisA SO2069 0.88 -2.3
2,166,241 + -1.9
2,166,311 - -3.0
2,166,483 + hisH SO2070 0.11 -2.9
2,166,483 + hisH SO2070 0.11 -3.9
2,166,510 + hisH SO2070 0.15 -4.7
2,166,552 - hisH SO2070 0.21 -4.2
2,166,575 - hisH SO2070 0.25 -3.8

Or see this region's nucleotide sequence