Experiment: ShewMM_MES_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1486 and SO1487 are separated by 54 nucleotides SO1487 and SO1489 are separated by 489 nucleotides SO1489 and adhB are separated by 682 nucleotides
SO1486: SO1486 - cytosolic long-chain acyl-CoA thioester hydrolase family protein (NCBI ptt file), at 1,553,032 to 1,553,400
SO1486
SO1487: SO1487 - hypothetical protein (NCBI ptt file), at 1,553,455 to 1,553,667
SO1487
SO1489: SO1489 - hypothetical protein (NCBI ptt file), at 1,554,157 to 1,554,564
SO1489
SO1490: adhB - alcohol dehydrogenase II (NCBI ptt file), at 1,555,247 to 1,556,395
adhB
Position (kb)
1554
1555 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1553.204 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.252 kb on + strand, within SO1486 at 1553.260 kb on - strand, within SO1486 at 1553.287 kb on - strand, within SO1486 at 1553.295 kb on - strand, within SO1486 at 1553.339 kb on - strand, within SO1486 at 1553.428 kb on - strand at 1553.439 kb on + strand at 1553.619 kb on - strand, within SO1487 at 1553.632 kb on - strand, within SO1487 at 1553.642 kb on + strand, within SO1487 at 1553.643 kb on + strand, within SO1487 at 1553.747 kb on + strand at 1553.755 kb on - strand at 1554.028 kb on + strand at 1554.059 kb on + strand at 1554.067 kb on - strand at 1554.162 kb on - strand at 1554.163 kb on - strand at 1554.163 kb on - strand at 1554.173 kb on - strand at 1554.183 kb on - strand at 1554.252 kb on - strand, within SO1489 at 1554.385 kb on - strand, within SO1489 at 1554.452 kb on - strand, within SO1489 at 1554.488 kb on + strand, within SO1489 at 1554.604 kb on + strand at 1554.673 kb on + strand at 1554.728 kb on + strand at 1554.796 kb on - strand at 1554.855 kb on + strand at 1554.875 kb on - strand at 1554.889 kb on + strand at 1555.149 kb on + strand at 1555.244 kb on - strand at 1555.302 kb on - strand at 1555.321 kb on + strand at 1555.476 kb on - strand, within adhB at 1555.515 kb on + strand, within adhB at 1555.531 kb on + strand, within adhB
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM_MES_buffer remove 1,553,204 + SO1486 0.47 +0.2 1,553,252 + SO1486 0.60 -0.2 1,553,252 + SO1486 0.60 -0.8 1,553,252 + SO1486 0.60 +0.0 1,553,252 + SO1486 0.60 +1.9 1,553,260 - SO1486 0.62 +0.1 1,553,287 - SO1486 0.69 -0.3 1,553,295 - SO1486 0.71 -0.3 1,553,339 - SO1486 0.83 +0.7 1,553,428 - -0.3 1,553,439 + +0.1 1,553,619 - SO1487 0.77 -0.5 1,553,632 - SO1487 0.83 -2.1 1,553,642 + SO1487 0.88 +1.1 1,553,643 + SO1487 0.88 +0.5 1,553,747 + +1.1 1,553,755 - -0.2 1,554,028 + -0.2 1,554,059 + +0.4 1,554,067 - -0.5 1,554,162 - +0.5 1,554,163 - +0.1 1,554,163 - -0.2 1,554,173 - -0.2 1,554,183 - +0.6 1,554,252 - SO1489 0.23 -0.1 1,554,385 - SO1489 0.56 +1.1 1,554,452 - SO1489 0.72 +0.3 1,554,488 + SO1489 0.81 -0.0 1,554,604 + +0.1 1,554,673 + -0.3 1,554,728 + +0.1 1,554,796 - -0.7 1,554,855 + +0.7 1,554,875 - +0.5 1,554,889 + -1.1 1,555,149 + -0.4 1,555,244 - -0.1 1,555,302 - +0.2 1,555,321 + +0.7 1,555,476 - adhB SO1490 0.20 +0.1 1,555,515 + adhB SO1490 0.23 +1.2 1,555,531 + adhB SO1490 0.25 +0.3
Or see this region's nucleotide sequence