Experiment: ShewMM_MES_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1046 and SO1047 are separated by 169 nucleotides SO1047 and SO1048 overlap by 1 nucleotides SO1048 and SO1049 are separated by 397 nucleotides
SO1046: SO1046 - transcriptional regulator, LysR family (NCBI ptt file), at 1,084,167 to 1,085,057
SO1046
SO1047: SO1047 - conserved hypothetical protein (NCBI ptt file), at 1,085,227 to 1,085,613
SO1047
SO1048: SO1048 - membrane protein, putative (NCBI ptt file), at 1,085,613 to 1,086,347
SO1048
SO1049: SO1049 - acetyltransferase, GNAT family (NCBI ptt file), at 1,086,745 to 1,087,221
SO1049
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1084.663 kb on + strand, within SO1046 at 1084.749 kb on + strand, within SO1046 at 1084.749 kb on + strand, within SO1046 at 1084.749 kb on + strand, within SO1046 at 1084.757 kb on + strand, within SO1046 at 1084.757 kb on - strand, within SO1046 at 1084.765 kb on - strand, within SO1046 at 1084.765 kb on - strand, within SO1046 at 1084.769 kb on + strand, within SO1046 at 1084.769 kb on + strand, within SO1046 at 1084.792 kb on - strand, within SO1046 at 1084.842 kb on - strand, within SO1046 at 1084.873 kb on + strand, within SO1046 at 1084.873 kb on + strand, within SO1046 at 1084.927 kb on - strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.954 kb on + strand, within SO1046 at 1084.962 kb on - strand, within SO1046 at 1084.962 kb on - strand, within SO1046 at 1084.969 kb on + strand at 1084.995 kb on + strand at 1085.302 kb on + strand, within SO1047 at 1085.304 kb on + strand, within SO1047 at 1085.310 kb on - strand, within SO1047 at 1085.314 kb on + strand, within SO1047 at 1085.314 kb on + strand, within SO1047 at 1085.322 kb on - strand, within SO1047 at 1085.322 kb on - strand, within SO1047 at 1085.322 kb on - strand, within SO1047 at 1085.379 kb on + strand, within SO1047 at 1085.379 kb on + strand, within SO1047 at 1085.459 kb on + strand, within SO1047 at 1085.467 kb on - strand, within SO1047 at 1085.492 kb on + strand, within SO1047 at 1085.494 kb on - strand, within SO1047 at 1085.505 kb on - strand, within SO1047 at 1085.505 kb on - strand, within SO1047 at 1085.505 kb on - strand, within SO1047 at 1085.515 kb on - strand, within SO1047 at 1085.515 kb on - strand, within SO1047 at 1085.539 kb on - strand, within SO1047 at 1085.598 kb on + strand at 1085.598 kb on + strand at 1085.598 kb on + strand at 1085.598 kb on + strand at 1085.606 kb on - strand at 1085.606 kb on - strand at 1085.606 kb on - strand at 1085.606 kb on - strand at 1085.606 kb on - strand at 1085.616 kb on - strand at 1085.628 kb on - strand at 1085.745 kb on + strand, within SO1048 at 1085.753 kb on - strand, within SO1048 at 1085.753 kb on - strand, within SO1048 at 1085.805 kb on - strand, within SO1048 at 1085.857 kb on + strand, within SO1048 at 1085.911 kb on - strand, within SO1048 at 1085.973 kb on + strand, within SO1048 at 1085.973 kb on + strand, within SO1048 at 1085.980 kb on - strand, within SO1048 at 1086.124 kb on - strand, within SO1048 at 1086.246 kb on + strand, within SO1048 at 1086.308 kb on + strand at 1086.308 kb on + strand at 1086.471 kb on - strand at 1086.482 kb on + strand at 1086.557 kb on + strand at 1086.647 kb on + strand at 1086.662 kb on - strand at 1086.662 kb on - strand at 1086.793 kb on - strand, within SO1049 at 1086.976 kb on - strand, within SO1049 at 1087.032 kb on - strand, within SO1049 at 1087.079 kb on + strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.087 kb on - strand, within SO1049 at 1087.110 kb on + strand, within SO1049 at 1087.213 kb on - strand at 1087.213 kb on - strand at 1087.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM_MES_buffer remove 1,084,663 + SO1046 0.56 -0.3 1,084,749 + SO1046 0.65 -0.3 1,084,749 + SO1046 0.65 +0.2 1,084,749 + SO1046 0.65 +0.3 1,084,757 + SO1046 0.66 +0.5 1,084,757 - SO1046 0.66 +0.2 1,084,765 - SO1046 0.67 -0.4 1,084,765 - SO1046 0.67 -0.1 1,084,769 + SO1046 0.68 +0.4 1,084,769 + SO1046 0.68 +0.2 1,084,792 - SO1046 0.70 +0.4 1,084,842 - SO1046 0.76 +0.0 1,084,873 + SO1046 0.79 +0.9 1,084,873 + SO1046 0.79 +0.2 1,084,927 - SO1046 0.85 +0.3 1,084,954 + SO1046 0.88 -1.1 1,084,954 + SO1046 0.88 +0.3 1,084,954 + SO1046 0.88 +0.8 1,084,954 + SO1046 0.88 +0.4 1,084,954 + SO1046 0.88 -0.3 1,084,954 + SO1046 0.88 -0.2 1,084,954 + SO1046 0.88 +0.3 1,084,962 - SO1046 0.89 +0.3 1,084,962 - SO1046 0.89 -1.3 1,084,969 + +0.6 1,084,995 + +0.8 1,085,302 + SO1047 0.19 -1.5 1,085,304 + SO1047 0.20 -1.2 1,085,310 - SO1047 0.21 -1.0 1,085,314 + SO1047 0.22 -0.3 1,085,314 + SO1047 0.22 -2.0 1,085,322 - SO1047 0.25 -4.0 1,085,322 - SO1047 0.25 -1.5 1,085,322 - SO1047 0.25 -1.3 1,085,379 + SO1047 0.39 -1.5 1,085,379 + SO1047 0.39 -0.1 1,085,459 + SO1047 0.60 -0.8 1,085,467 - SO1047 0.62 -0.7 1,085,492 + SO1047 0.68 -3.0 1,085,494 - SO1047 0.69 -2.1 1,085,505 - SO1047 0.72 -1.1 1,085,505 - SO1047 0.72 -1.1 1,085,505 - SO1047 0.72 -0.1 1,085,515 - SO1047 0.74 -1.3 1,085,515 - SO1047 0.74 -1.1 1,085,539 - SO1047 0.81 -0.9 1,085,598 + -0.7 1,085,598 + -1.4 1,085,598 + -1.2 1,085,598 + -1.3 1,085,606 - -0.6 1,085,606 - -0.6 1,085,606 - -2.2 1,085,606 - -2.7 1,085,606 - -1.4 1,085,616 - -1.7 1,085,628 - -1.0 1,085,745 + SO1048 0.18 -0.5 1,085,753 - SO1048 0.19 -1.1 1,085,753 - SO1048 0.19 +1.5 1,085,805 - SO1048 0.26 -1.9 1,085,857 + SO1048 0.33 -2.4 1,085,911 - SO1048 0.41 -1.3 1,085,973 + SO1048 0.49 -0.1 1,085,973 + SO1048 0.49 -1.3 1,085,980 - SO1048 0.50 -1.5 1,086,124 - SO1048 0.70 -2.3 1,086,246 + SO1048 0.86 -1.7 1,086,308 + -0.9 1,086,308 + -2.1 1,086,471 - -0.8 1,086,482 + -0.4 1,086,557 + -0.7 1,086,647 + +0.2 1,086,662 - -0.2 1,086,662 - -0.1 1,086,793 - SO1049 0.10 +1.6 1,086,976 - SO1049 0.48 -0.1 1,087,032 - SO1049 0.60 +0.1 1,087,079 + SO1049 0.70 +0.1 1,087,087 - SO1049 0.72 -0.6 1,087,087 - SO1049 0.72 +0.2 1,087,087 - SO1049 0.72 +0.6 1,087,087 - SO1049 0.72 +0.4 1,087,110 + SO1049 0.77 -1.1 1,087,213 - +0.7 1,087,213 - -1.5 1,087,314 - +1.0
Or see this region's nucleotide sequence