Strain Fitness in Shewanella oneidensis MR-1 around SO3964

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoN and yfiA-3 are separated by 28 nucleotidesyfiA-3 and ptsN are separated by 2 nucleotidesptsN and SO3964 overlap by 4 nucleotidesSO3964 and ptsO overlap by 14 nucleotidesptsO and mgtE-2 are separated by 196 nucleotides SO3961: rpoN - RNA polymerase sigma-54 factor (NCBI ptt file), at 4,102,103 to 4,103,578 rpoN SO3962: yfiA-3 - ribosomal subunit interface protein (NCBI ptt file), at 4,103,607 to 4,103,894 yfiA-3 SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340 ptsN SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191 SO3964 SO3965: ptsO - phosphocarrier protein NPR (NCBI ptt file), at 4,105,178 to 4,105,453 ptsO SO3966: mgtE-2 - magnesium transporter (NCBI ptt file), at 4,105,650 to 4,107,014 mgtE-2 Position (kb) 4104 4105 4106Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4103.365 kb on + strand, within rpoNat 4103.365 kb on + strand, within rpoNat 4103.373 kb on - strand, within rpoNat 4103.373 kb on - strand, within rpoNat 4103.383 kb on - strand, within rpoNat 4103.389 kb on + strand, within rpoNat 4103.389 kb on + strand, within rpoNat 4103.397 kb on - strand, within rpoNat 4103.487 kb on - strandat 4103.535 kb on - strandat 4103.561 kb on - strandat 4103.563 kb on + strandat 4103.563 kb on + strandat 4103.642 kb on - strand, within yfiA-3at 4103.657 kb on + strand, within yfiA-3at 4103.705 kb on + strand, within yfiA-3at 4103.823 kb on + strand, within yfiA-3at 4103.895 kb on + strandat 4103.942 kb on + strand, within ptsNat 4103.942 kb on + strand, within ptsNat 4103.950 kb on - strand, within ptsNat 4103.993 kb on + strand, within ptsNat 4104.001 kb on - strand, within ptsNat 4104.065 kb on + strand, within ptsNat 4104.065 kb on + strand, within ptsNat 4104.108 kb on + strand, within ptsNat 4104.278 kb on + strand, within ptsNat 4104.386 kb on + strandat 4104.394 kb on - strandat 4104.394 kb on - strandat 4104.465 kb on + strand, within SO3964at 4104.473 kb on - strand, within SO3964at 4104.473 kb on - strand, within SO3964at 4104.574 kb on - strand, within SO3964at 4104.942 kb on - strand, within SO3964at 4105.286 kb on - strand, within ptsOat 4105.421 kb on + strand, within ptsOat 4105.443 kb on - strandat 4105.654 kb on - strandat 4105.680 kb on - strandat 4105.906 kb on + strand, within mgtE-2at 4105.914 kb on - strand, within mgtE-2at 4106.006 kb on + strand, within mgtE-2at 4106.048 kb on + strand, within mgtE-2at 4106.056 kb on - strand, within mgtE-2at 4106.082 kb on - strand, within mgtE-2at 4106.165 kb on - strand, within mgtE-2

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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4,103,365 + rpoN SO3961 0.86 -2.2
4,103,365 + rpoN SO3961 0.86 -3.5
4,103,373 - rpoN SO3961 0.86 -1.8
4,103,373 - rpoN SO3961 0.86 +0.4
4,103,383 - rpoN SO3961 0.87 -2.9
4,103,389 + rpoN SO3961 0.87 -3.8
4,103,389 + rpoN SO3961 0.87 -3.5
4,103,397 - rpoN SO3961 0.88 -2.1
4,103,487 - -2.7
4,103,535 - -4.0
4,103,561 - -2.1
4,103,563 + -1.0
4,103,563 + +0.1
4,103,642 - yfiA-3 SO3962 0.12 -0.5
4,103,657 + yfiA-3 SO3962 0.17 -0.5
4,103,705 + yfiA-3 SO3962 0.34 -3.1
4,103,823 + yfiA-3 SO3962 0.75 -0.4
4,103,895 + +0.6
4,103,942 + ptsN SO3963 0.10 -3.7
4,103,942 + ptsN SO3963 0.10 -0.7
4,103,950 - ptsN SO3963 0.12 -2.2
4,103,993 + ptsN SO3963 0.22 -2.4
4,104,001 - ptsN SO3963 0.23 -0.9
4,104,065 + ptsN SO3963 0.38 -1.8
4,104,065 + ptsN SO3963 0.38 -0.2
4,104,108 + ptsN SO3963 0.48 -1.9
4,104,278 + ptsN SO3963 0.86 -0.2
4,104,386 + -1.3
4,104,394 - -2.5
4,104,394 - -3.4
4,104,465 + SO3964 0.15 -0.9
4,104,473 - SO3964 0.16 -1.9
4,104,473 - SO3964 0.16 -2.6
4,104,574 - SO3964 0.28 -2.6
4,104,942 - SO3964 0.71 -2.0
4,105,286 - ptsO SO3965 0.39 -0.3
4,105,421 + ptsO SO3965 0.88 -0.3
4,105,443 - -0.2
4,105,654 - -0.0
4,105,680 - -1.6
4,105,906 + mgtE-2 SO3966 0.19 +1.7
4,105,914 - mgtE-2 SO3966 0.19 -0.0
4,106,006 + mgtE-2 SO3966 0.26 +1.1
4,106,048 + mgtE-2 SO3966 0.29 -0.9
4,106,056 - mgtE-2 SO3966 0.30 +0.4
4,106,082 - mgtE-2 SO3966 0.32 -1.3
4,106,165 - mgtE-2 SO3966 0.38 -0.1

Or see this region's nucleotide sequence