Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panC and panB are separated by 76 nucleotides panB and folK-1 are separated by 56 nucleotides folK-1 and pcnB overlap by 1 nucleotides
SO0869: panC - pantoate--beta-alanine ligase (NCBI ptt file), at 899,247 to 900,092
panC
SO0870: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI ptt file), at 900,169 to 900,963
panB
SO0871: folK-1 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 901,020 to 901,508
folK-1
SO0872: pcnB - polyA polymerase (NCBI ptt file), at 901,508 to 902,824
pcnB
Position (kb) 900
901 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 899.198 kb on - strand at 899.198 kb on - strand at 899.198 kb on - strand at 900.166 kb on - strand at 900.357 kb on - strand, within panB at 900.551 kb on - strand, within panB at 900.740 kb on - strand, within panB at 900.800 kb on - strand, within panB at 900.947 kb on - strand at 900.962 kb on - strand at 901.040 kb on - strand at 901.074 kb on + strand, within folK-1 at 901.571 kb on - strand at 901.752 kb on - strand, within pcnB
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 899,198 - +0.3 899,198 - -1.1 899,198 - -0.1 900,166 - -1.0 900,357 - panB SO0870 0.24 -3.3 900,551 - panB SO0870 0.48 -5.2 900,740 - panB SO0870 0.72 -2.8 900,800 - panB SO0870 0.79 -3.7 900,947 - -2.0 900,962 - -4.2 901,040 - -5.1 901,074 + folK-1 SO0871 0.11 -3.3 901,571 - +0.3 901,752 - pcnB SO0872 0.19 -0.2
Or see this region's nucleotide sequence