Experiment: m.b. 25C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_324 and HP15_325 are separated by 59 nucleotides HP15_325 and HP15_326 overlap by 4 nucleotides HP15_326 and HP15_327 are separated by 28 nucleotides HP15_327 and HP15_328 are separated by 469 nucleotides
HP15_324: HP15_324 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 320,847 to 321,641
_324
HP15_325: HP15_325 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 321,701 to 322,198
_325
HP15_326: HP15_326 - polyA polymerase, at 322,195 to 323,526
_326
HP15_327: HP15_327 - hypothetical protein, at 323,555 to 323,764
_327
HP15_328: HP15_328 - two component, sigma54 specific, transcriptional regulator, Fis family, at 324,234 to 325,637
_328
Position (kb) 322
323
324 Strain fitness (log2 ratio) -2
-1
0
1 at 321.670 kb on - strand at 322.261 kb on - strand at 322.273 kb on - strand at 322.293 kb on - strand at 322.382 kb on + strand, within HP15_326 at 322.562 kb on - strand, within HP15_326 at 322.573 kb on - strand, within HP15_326 at 322.577 kb on - strand, within HP15_326 at 322.604 kb on + strand, within HP15_326 at 322.746 kb on - strand, within HP15_326 at 322.748 kb on - strand, within HP15_326 at 322.753 kb on - strand, within HP15_326 at 322.789 kb on + strand, within HP15_326 at 322.789 kb on + strand, within HP15_326 at 322.816 kb on - strand, within HP15_326 at 322.955 kb on + strand, within HP15_326 at 323.110 kb on + strand, within HP15_326 at 323.118 kb on - strand, within HP15_326 at 323.200 kb on - strand, within HP15_326 at 323.503 kb on - strand at 323.659 kb on - strand, within HP15_327 at 323.789 kb on - strand at 324.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 25C remove 321,670 - +1.6 322,261 - -0.5 322,273 - +1.0 322,293 - +0.2 322,382 + HP15_326 0.14 -1.4 322,562 - HP15_326 0.28 -0.9 322,573 - HP15_326 0.28 +0.1 322,577 - HP15_326 0.29 +0.4 322,604 + HP15_326 0.31 +0.1 322,746 - HP15_326 0.41 -0.8 322,748 - HP15_326 0.42 -0.1 322,753 - HP15_326 0.42 -0.6 322,789 + HP15_326 0.45 -0.4 322,789 + HP15_326 0.45 -2.7 322,816 - HP15_326 0.47 -1.0 322,955 + HP15_326 0.57 -1.8 323,110 + HP15_326 0.69 +0.1 323,118 - HP15_326 0.69 -0.2 323,200 - HP15_326 0.75 -0.3 323,503 - +0.8 323,659 - HP15_327 0.50 -1.2 323,789 - -0.0 324,122 + +0.2
Or see this region's nucleotide sequence