Experiment: ShewMM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hpt-2 and ubiX are separated by 7 nucleotides ubiX and mpl are separated by 1 nucleotides mpl and SO3807 are separated by 311 nucleotides
SO3803: hpt-2 - hypoxanthine phosphoribosyltransferase (NCBI ptt file), at 3,958,012 to 3,958,542
hpt-2
SO3804: ubiX - phenylacrylic acid decarboxylase, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, putative (NCBI ptt file), at 3,958,550 to 3,959,209
ubiX
SO3805: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (NCBI ptt file), at 3,959,211 to 3,960,608
mpl
SO3807: SO3807 - sterol desaturase family protein (NCBI ptt file), at 3,960,920 to 3,961,783
SO3807
Position (kb)
3959
3960
3961 Strain fitness (log2 ratio)
-2
-1
0
1 at 3958.321 kb on + strand, within hpt-2 at 3958.329 kb on - strand, within hpt-2 at 3958.524 kb on - strand at 3959.212 kb on - strand at 3959.212 kb on - strand at 3959.212 kb on - strand at 3959.450 kb on - strand, within mpl at 3959.571 kb on - strand, within mpl at 3959.738 kb on - strand, within mpl at 3959.762 kb on - strand, within mpl at 3959.872 kb on - strand, within mpl at 3959.872 kb on - strand, within mpl at 3959.872 kb on - strand, within mpl at 3960.500 kb on - strand at 3960.650 kb on - strand at 3960.671 kb on + strand at 3960.671 kb on + strand at 3960.801 kb on - strand at 3960.815 kb on - strand at 3960.845 kb on + strand at 3960.845 kb on + strand at 3960.846 kb on + strand at 3960.846 kb on + strand at 3960.846 kb on + strand at 3960.847 kb on + strand at 3960.852 kb on + strand at 3960.855 kb on - strand at 3960.860 kb on - strand at 3960.980 kb on + strand at 3960.993 kb on + strand at 3961.002 kb on + strand at 3961.010 kb on - strand, within SO3807 at 3961.062 kb on + strand, within SO3807 at 3961.188 kb on - strand, within SO3807 at 3961.188 kb on - strand, within SO3807 at 3961.198 kb on - strand, within SO3807 at 3961.203 kb on + strand, within SO3807 at 3961.203 kb on + strand, within SO3807 at 3961.211 kb on - strand, within SO3807 at 3961.360 kb on + strand, within SO3807 at 3961.364 kb on + strand, within SO3807 at 3961.375 kb on + strand, within SO3807 at 3961.420 kb on - strand, within SO3807 at 3961.494 kb on + strand, within SO3807 at 3961.494 kb on + strand, within SO3807 at 3961.499 kb on + strand, within SO3807 at 3961.512 kb on - strand, within SO3807 at 3961.599 kb on - strand, within SO3807
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM remove 3,958,321 + hpt-2 SO3803 0.58 +0.4 3,958,329 - hpt-2 SO3803 0.60 -0.4 3,958,524 - +0.1 3,959,212 - -0.2 3,959,212 - -1.1 3,959,212 - -1.0 3,959,450 - mpl SO3805 0.17 -1.4 3,959,571 - mpl SO3805 0.26 -0.1 3,959,738 - mpl SO3805 0.38 +0.1 3,959,762 - mpl SO3805 0.39 -2.6 3,959,872 - mpl SO3805 0.47 -2.0 3,959,872 - mpl SO3805 0.47 -2.0 3,959,872 - mpl SO3805 0.47 -1.6 3,960,500 - +0.8 3,960,650 - -0.1 3,960,671 + +0.6 3,960,671 + +0.1 3,960,801 - +0.3 3,960,815 - -1.4 3,960,845 + +0.4 3,960,845 + +0.9 3,960,846 + -0.6 3,960,846 + -0.7 3,960,846 + -0.0 3,960,847 + +0.4 3,960,852 + +0.3 3,960,855 - -0.0 3,960,860 - +0.2 3,960,980 + +0.1 3,960,993 + +0.1 3,961,002 + -1.0 3,961,010 - SO3807 0.10 +0.5 3,961,062 + SO3807 0.16 +0.0 3,961,188 - SO3807 0.31 +0.1 3,961,188 - SO3807 0.31 +0.3 3,961,198 - SO3807 0.32 -0.4 3,961,203 + SO3807 0.33 -0.1 3,961,203 + SO3807 0.33 -0.1 3,961,211 - SO3807 0.34 +0.1 3,961,360 + SO3807 0.51 -0.0 3,961,364 + SO3807 0.51 -0.3 3,961,375 + SO3807 0.53 -0.0 3,961,420 - SO3807 0.58 +0.3 3,961,494 + SO3807 0.66 -0.3 3,961,494 + SO3807 0.66 +0.5 3,961,499 + SO3807 0.67 +0.3 3,961,512 - SO3807 0.69 -0.2 3,961,599 - SO3807 0.79 +0.3
Or see this region's nucleotide sequence