Experiment: Cholin acetate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgE and flgD are separated by 33 nucleotides flgD and flgC are separated by 26 nucleotides flgC and flgB are separated by 2 nucleotides flgB and Sama_2319 are separated by 141 nucleotides
Sama_2315: flgE - flagellar hook protein FlgE (RefSeq), at 2,780,894 to 2,782,255
flgE
Sama_2316: flgD - flagellar basal body rod modification protein (RefSeq), at 2,782,289 to 2,783,017
flgD
Sama_2317: flgC - flagellar basal body rod protein FlgC (RefSeq), at 2,783,044 to 2,783,457
flgC
Sama_2318: flgB - flagellar basal body rod protein FlgB (RefSeq), at 2,783,460 to 2,783,858
flgB
Sama_2319: Sama_2319 - protein-glutamate O-methyltransferase (RefSeq), at 2,784,000 to 2,784,833
_2319
Position (kb)
2783
2784 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2782.080 kb on - strand, within flgE at 2782.116 kb on - strand, within flgE at 2782.128 kb on + strand at 2782.128 kb on + strand at 2782.129 kb on - strand at 2782.129 kb on - strand at 2782.255 kb on + strand at 2782.256 kb on - strand at 2782.256 kb on - strand at 2782.256 kb on - strand at 2782.273 kb on - strand at 2782.401 kb on + strand, within flgD at 2782.402 kb on - strand, within flgD at 2782.402 kb on - strand, within flgD at 2782.402 kb on - strand, within flgD at 2782.402 kb on - strand, within flgD at 2782.416 kb on - strand, within flgD at 2782.461 kb on + strand, within flgD at 2782.461 kb on + strand, within flgD at 2782.461 kb on + strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.462 kb on - strand, within flgD at 2782.465 kb on + strand, within flgD at 2782.466 kb on - strand, within flgD at 2782.466 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.653 kb on - strand, within flgD at 2782.680 kb on - strand, within flgD at 2782.680 kb on - strand, within flgD at 2782.680 kb on - strand, within flgD at 2782.736 kb on - strand, within flgD at 2782.736 kb on - strand, within flgD at 2783.000 kb on - strand at 2783.006 kb on - strand at 2783.022 kb on + strand at 2783.023 kb on - strand at 2783.077 kb on - strand at 2783.077 kb on - strand at 2783.077 kb on - strand at 2783.178 kb on + strand, within flgC at 2783.217 kb on - strand, within flgC at 2783.235 kb on + strand, within flgC at 2783.236 kb on - strand, within flgC at 2783.248 kb on + strand, within flgC at 2783.248 kb on + strand, within flgC at 2783.249 kb on - strand, within flgC at 2783.334 kb on - strand, within flgC at 2783.446 kb on - strand at 2783.472 kb on - strand at 2783.474 kb on + strand at 2783.487 kb on - strand at 2783.547 kb on - strand, within flgB at 2783.547 kb on - strand, within flgB at 2783.582 kb on + strand, within flgB at 2783.582 kb on + strand, within flgB at 2783.583 kb on - strand, within flgB at 2783.583 kb on - strand, within flgB at 2783.583 kb on - strand, within flgB at 2783.745 kb on - strand, within flgB at 2783.745 kb on - strand, within flgB at 2783.747 kb on - strand, within flgB at 2783.747 kb on - strand, within flgB at 2783.928 kb on - strand at 2783.928 kb on - strand at 2783.928 kb on - strand at 2784.020 kb on - strand at 2784.071 kb on + strand at 2784.071 kb on + strand at 2784.072 kb on - strand at 2784.072 kb on - strand at 2784.136 kb on - strand, within Sama_2319 at 2784.164 kb on - strand, within Sama_2319 at 2784.200 kb on + strand, within Sama_2319 at 2784.214 kb on + strand, within Sama_2319 at 2784.215 kb on - strand, within Sama_2319 at 2784.215 kb on - strand, within Sama_2319 at 2784.230 kb on + strand, within Sama_2319 at 2784.230 kb on + strand, within Sama_2319 at 2784.230 kb on + strand, within Sama_2319 at 2784.230 kb on + strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.231 kb on - strand, within Sama_2319 at 2784.270 kb on + strand, within Sama_2319 at 2784.279 kb on - strand, within Sama_2319 at 2784.356 kb on - strand, within Sama_2319 at 2784.356 kb on - strand, within Sama_2319 at 2784.368 kb on + strand, within Sama_2319 at 2784.368 kb on + strand, within Sama_2319 at 2784.368 kb on + strand, within Sama_2319
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 25 mM remove 2,782,080 - flgE Sama_2315 0.87 -1.6 2,782,116 - flgE Sama_2315 0.90 +1.0 2,782,128 + -0.6 2,782,128 + -0.8 2,782,129 - -1.0 2,782,129 - -0.0 2,782,255 + -1.0 2,782,256 - -1.2 2,782,256 - -0.4 2,782,256 - +1.7 2,782,273 - +0.7 2,782,401 + flgD Sama_2316 0.15 +1.0 2,782,402 - flgD Sama_2316 0.16 -0.8 2,782,402 - flgD Sama_2316 0.16 -0.0 2,782,402 - flgD Sama_2316 0.16 -1.0 2,782,402 - flgD Sama_2316 0.16 +2.6 2,782,416 - flgD Sama_2316 0.17 -0.0 2,782,461 + flgD Sama_2316 0.24 +1.0 2,782,461 + flgD Sama_2316 0.24 -1.8 2,782,461 + flgD Sama_2316 0.24 +0.4 2,782,462 - flgD Sama_2316 0.24 -0.8 2,782,462 - flgD Sama_2316 0.24 -1.8 2,782,462 - flgD Sama_2316 0.24 +0.6 2,782,462 - flgD Sama_2316 0.24 -0.4 2,782,462 - flgD Sama_2316 0.24 +0.4 2,782,462 - flgD Sama_2316 0.24 -0.7 2,782,462 - flgD Sama_2316 0.24 -1.0 2,782,465 + flgD Sama_2316 0.24 +0.6 2,782,466 - flgD Sama_2316 0.24 -0.4 2,782,466 - flgD Sama_2316 0.24 -1.8 2,782,653 - flgD Sama_2316 0.50 -0.8 2,782,653 - flgD Sama_2316 0.50 -0.5 2,782,653 - flgD Sama_2316 0.50 +0.1 2,782,653 - flgD Sama_2316 0.50 +0.5 2,782,653 - flgD Sama_2316 0.50 -0.4 2,782,653 - flgD Sama_2316 0.50 +0.6 2,782,653 - flgD Sama_2316 0.50 -0.5 2,782,653 - flgD Sama_2316 0.50 -0.4 2,782,653 - flgD Sama_2316 0.50 +0.7 2,782,680 - flgD Sama_2316 0.54 +0.4 2,782,680 - flgD Sama_2316 0.54 -0.6 2,782,680 - flgD Sama_2316 0.54 -0.8 2,782,736 - flgD Sama_2316 0.61 +0.8 2,782,736 - flgD Sama_2316 0.61 -0.8 2,783,000 - -0.0 2,783,006 - -0.1 2,783,022 + -1.5 2,783,023 - +0.4 2,783,077 - -1.6 2,783,077 - +0.6 2,783,077 - +0.2 2,783,178 + flgC Sama_2317 0.32 +0.4 2,783,217 - flgC Sama_2317 0.42 -0.3 2,783,235 + flgC Sama_2317 0.46 +1.2 2,783,236 - flgC Sama_2317 0.46 +0.6 2,783,248 + flgC Sama_2317 0.49 -0.6 2,783,248 + flgC Sama_2317 0.49 +1.0 2,783,249 - flgC Sama_2317 0.50 -0.8 2,783,334 - flgC Sama_2317 0.70 -0.0 2,783,446 - +0.6 2,783,472 - +0.7 2,783,474 + -0.0 2,783,487 - -1.4 2,783,547 - flgB Sama_2318 0.22 +1.0 2,783,547 - flgB Sama_2318 0.22 +2.3 2,783,582 + flgB Sama_2318 0.31 +1.0 2,783,582 + flgB Sama_2318 0.31 -0.3 2,783,583 - flgB Sama_2318 0.31 +0.4 2,783,583 - flgB Sama_2318 0.31 +0.5 2,783,583 - flgB Sama_2318 0.31 -0.6 2,783,745 - flgB Sama_2318 0.71 -2.2 2,783,745 - flgB Sama_2318 0.71 +1.0 2,783,747 - flgB Sama_2318 0.72 +0.7 2,783,747 - flgB Sama_2318 0.72 +0.4 2,783,928 - -0.1 2,783,928 - +1.0 2,783,928 - +1.6 2,784,020 - +1.0 2,784,071 + -0.8 2,784,071 + -0.0 2,784,072 - -0.5 2,784,072 - +0.6 2,784,136 - Sama_2319 0.16 -2.0 2,784,164 - Sama_2319 0.20 -0.2 2,784,200 + Sama_2319 0.24 +0.6 2,784,214 + Sama_2319 0.26 -1.6 2,784,215 - Sama_2319 0.26 -0.8 2,784,215 - Sama_2319 0.26 -1.1 2,784,230 + Sama_2319 0.28 +1.0 2,784,230 + Sama_2319 0.28 +0.4 2,784,230 + Sama_2319 0.28 -1.7 2,784,230 + Sama_2319 0.28 -0.4 2,784,231 - Sama_2319 0.28 -0.8 2,784,231 - Sama_2319 0.28 +1.2 2,784,231 - Sama_2319 0.28 -1.7 2,784,231 - Sama_2319 0.28 -0.9 2,784,231 - Sama_2319 0.28 -1.1 2,784,231 - Sama_2319 0.28 -1.6 2,784,231 - Sama_2319 0.28 +1.3 2,784,231 - Sama_2319 0.28 +1.0 2,784,270 + Sama_2319 0.32 +0.7 2,784,279 - Sama_2319 0.33 -0.8 2,784,356 - Sama_2319 0.43 -0.4 2,784,356 - Sama_2319 0.43 -1.0 2,784,368 + Sama_2319 0.44 -2.5 2,784,368 + Sama_2319 0.44 -0.3 2,784,368 + Sama_2319 0.44 +0.7
Or see this region's nucleotide sequence