Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4173 and Dshi_4174 are separated by 70 nucleotides Dshi_4174 and Dshi_4175 are separated by 63 nucleotides Dshi_4175 and Dshi_4176 are separated by 163 nucleotides Dshi_4176 and Dshi_4177 are separated by 91 nucleotides
Dshi_4173: Dshi_4173 - hypothetical protein (RefSeq), at 22,428 to 23,387
_4173
Dshi_4174: Dshi_4174 - hypothetical protein (RefSeq), at 23,458 to 24,015
_4174
Dshi_4175: Dshi_4175 - hypothetical protein (RefSeq), at 24,079 to 25,098
_4175
Dshi_4176: Dshi_4176 - oxidoreductase FAD/NAD(P)-binding domain protein (RefSeq), at 25,262 to 25,924
_4176
Dshi_4177: Dshi_4177 - Acetamidase/Formamidase (RefSeq), at 26,016 to 27,332
_4177
Position (kb) 24
25
26 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 23.679 kb on + strand, within Dshi_4174 at 23.708 kb on + strand, within Dshi_4174 at 23.848 kb on + strand, within Dshi_4174 at 23.856 kb on - strand, within Dshi_4174 at 24.157 kb on - strand at 24.157 kb on - strand at 24.359 kb on + strand, within Dshi_4175 at 24.443 kb on + strand, within Dshi_4175 at 25.139 kb on - strand at 25.159 kb on + strand at 25.448 kb on - strand at 25.501 kb on + strand, within Dshi_4176 at 25.714 kb on - strand, within Dshi_4176 at 25.745 kb on - strand, within Dshi_4176 at 25.999 kb on + strand at 26.002 kb on - strand at 26.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 23,679 + Dshi_4174 0.40 -1.2 23,708 + Dshi_4174 0.45 -0.9 23,848 + Dshi_4174 0.70 +0.7 23,856 - Dshi_4174 0.71 -1.4 24,157 - +0.8 24,157 - -1.5 24,359 + Dshi_4175 0.27 -0.5 24,443 + Dshi_4175 0.36 +1.4 25,139 - -1.8 25,159 + +2.1 25,448 - -0.2 25,501 + Dshi_4176 0.36 -0.5 25,714 - Dshi_4176 0.68 +1.8 25,745 - Dshi_4176 0.73 -0.6 25,999 + +3.1 26,002 - +0.1 26,073 - -2.8
Or see this region's nucleotide sequence