Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3095 and Dshi_3096 are separated by 2 nucleotides Dshi_3096 and Dshi_3097 are separated by 16 nucleotides Dshi_3097 and Dshi_3098 are separated by 35 nucleotides Dshi_3098 and Dshi_3099 overlap by 4 nucleotides
Dshi_3095: Dshi_3095 - protein of unknown function DUF205 (RefSeq), at 3,263,379 to 3,263,990
_3095
Dshi_3096: Dshi_3096 - protein of unknown function DUF805 (RefSeq), at 3,263,993 to 3,264,499
_3096
Dshi_3097: Dshi_3097 - glutamate--cysteine ligase (RefSeq), at 3,264,516 to 3,265,886
_3097
Dshi_3098: Dshi_3098 - hypothetical protein (RefSeq), at 3,265,922 to 3,266,470
_3098
Dshi_3099: Dshi_3099 - protein of unknown function DUF558 (RefSeq), at 3,266,467 to 3,267,216
_3099
Position (kb)
3264
3265
3266 Strain fitness (log2 ratio)
-2
-1
0
1 at 3263.998 kb on - strand at 3264.004 kb on - strand at 3264.530 kb on - strand at 3264.825 kb on - strand, within Dshi_3097 at 3265.898 kb on - strand at 3266.332 kb on - strand, within Dshi_3098 at 3266.332 kb on - strand, within Dshi_3098 at 3266.401 kb on + strand, within Dshi_3098 at 3266.401 kb on + strand, within Dshi_3098 at 3266.401 kb on + strand, within Dshi_3098 at 3266.409 kb on - strand, within Dshi_3098 at 3266.506 kb on - strand at 3266.572 kb on - strand, within Dshi_3099 at 3266.711 kb on + strand, within Dshi_3099
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 3,263,998 - -2.1 3,264,004 - +1.1 3,264,530 - -1.5 3,264,825 - Dshi_3097 0.23 +1.1 3,265,898 - +0.6 3,266,332 - Dshi_3098 0.75 +0.7 3,266,332 - Dshi_3098 0.75 +0.6 3,266,401 + Dshi_3098 0.87 +1.1 3,266,401 + Dshi_3098 0.87 -0.9 3,266,401 + Dshi_3098 0.87 -0.5 3,266,409 - Dshi_3098 0.89 +1.1 3,266,506 - +0.8 3,266,572 - Dshi_3099 0.14 +0.5 3,266,711 + Dshi_3099 0.33 -1.2
Or see this region's nucleotide sequence