Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2365 and Dshi_2366 are separated by 135 nucleotides Dshi_2366 and Dshi_2367 are separated by 112 nucleotides Dshi_2367 and Dshi_2368 overlap by 1 nucleotides
Dshi_2365: Dshi_2365 - urease, alpha subunit (RefSeq), at 2,498,640 to 2,500,349
_2365
Dshi_2366: Dshi_2366 - hypothetical protein (RefSeq), at 2,500,485 to 2,500,814
_2366
Dshi_2367: Dshi_2367 - alginate regulatory protein AlgP (RefSeq), at 2,500,927 to 2,501,649
_2367
Dshi_2368: Dshi_2368 - urease, beta subunit (RefSeq), at 2,501,649 to 2,501,954
_2368
Position (kb)
2500
2501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2499.520 kb on + strand, within Dshi_2365 at 2499.552 kb on - strand, within Dshi_2365 at 2499.557 kb on - strand, within Dshi_2365 at 2499.598 kb on - strand, within Dshi_2365 at 2499.607 kb on - strand, within Dshi_2365 at 2499.676 kb on + strand, within Dshi_2365 at 2499.920 kb on + strand, within Dshi_2365 at 2500.082 kb on + strand, within Dshi_2365 at 2500.082 kb on + strand, within Dshi_2365 at 2500.096 kb on - strand, within Dshi_2365 at 2500.168 kb on + strand, within Dshi_2365 at 2500.176 kb on - strand, within Dshi_2365 at 2500.178 kb on + strand, within Dshi_2365 at 2500.306 kb on + strand at 2500.345 kb on + strand at 2500.439 kb on + strand at 2500.491 kb on - strand at 2500.540 kb on - strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.679 kb on - strand, within Dshi_2366 at 2500.747 kb on + strand, within Dshi_2366 at 2500.750 kb on - strand, within Dshi_2366 at 2500.789 kb on + strand at 2500.791 kb on - strand at 2500.791 kb on - strand at 2500.797 kb on - strand at 2500.802 kb on - strand at 2500.802 kb on - strand at 2500.817 kb on - strand at 2500.843 kb on + strand at 2500.908 kb on + strand at 2500.913 kb on + strand at 2500.970 kb on - strand at 2500.979 kb on + strand at 2500.979 kb on + strand at 2500.987 kb on - strand at 2501.033 kb on - strand, within Dshi_2367 at 2501.145 kb on - strand, within Dshi_2367 at 2501.296 kb on - strand, within Dshi_2367 at 2501.306 kb on + strand, within Dshi_2367 at 2501.307 kb on - strand, within Dshi_2367 at 2501.357 kb on + strand, within Dshi_2367 at 2501.363 kb on + strand, within Dshi_2367 at 2501.421 kb on - strand, within Dshi_2367 at 2501.446 kb on + strand, within Dshi_2367 at 2501.471 kb on + strand, within Dshi_2367 at 2501.479 kb on - strand, within Dshi_2367 at 2501.479 kb on - strand, within Dshi_2367 at 2501.545 kb on - strand, within Dshi_2367 at 2501.614 kb on + strand at 2501.623 kb on + strand at 2501.623 kb on - strand at 2501.631 kb on - strand at 2501.698 kb on + strand, within Dshi_2368 at 2501.713 kb on + strand, within Dshi_2368 at 2501.789 kb on - strand, within Dshi_2368 at 2501.803 kb on + strand, within Dshi_2368
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 2,499,520 + Dshi_2365 0.51 +1.1 2,499,552 - Dshi_2365 0.53 +1.7 2,499,557 - Dshi_2365 0.54 +1.1 2,499,598 - Dshi_2365 0.56 +0.1 2,499,607 - Dshi_2365 0.57 +2.1 2,499,676 + Dshi_2365 0.61 -0.5 2,499,920 + Dshi_2365 0.75 -0.5 2,500,082 + Dshi_2365 0.84 -1.8 2,500,082 + Dshi_2365 0.84 +0.1 2,500,096 - Dshi_2365 0.85 +1.7 2,500,168 + Dshi_2365 0.89 +0.8 2,500,176 - Dshi_2365 0.90 -0.5 2,500,178 + Dshi_2365 0.90 -0.2 2,500,306 + +0.3 2,500,345 + +2.7 2,500,439 + +3.9 2,500,491 - +3.9 2,500,540 - Dshi_2366 0.17 +0.9 2,500,636 + Dshi_2366 0.46 +4.1 2,500,636 + Dshi_2366 0.46 +1.5 2,500,644 - Dshi_2366 0.48 -1.5 2,500,644 - Dshi_2366 0.48 -0.6 2,500,644 - Dshi_2366 0.48 -2.5 2,500,679 - Dshi_2366 0.59 -1.6 2,500,747 + Dshi_2366 0.79 +4.8 2,500,750 - Dshi_2366 0.80 -0.6 2,500,789 + -0.5 2,500,791 - +0.2 2,500,791 - -0.7 2,500,797 - -1.7 2,500,802 - +1.0 2,500,802 - +3.4 2,500,817 - -0.5 2,500,843 + +3.1 2,500,908 + -1.7 2,500,913 + +0.9 2,500,970 - -3.5 2,500,979 + +2.4 2,500,979 + +1.8 2,500,987 - -1.2 2,501,033 - Dshi_2367 0.15 -1.2 2,501,145 - Dshi_2367 0.30 +0.1 2,501,296 - Dshi_2367 0.51 -0.9 2,501,306 + Dshi_2367 0.52 -0.2 2,501,307 - Dshi_2367 0.53 +2.2 2,501,357 + Dshi_2367 0.59 +2.1 2,501,363 + Dshi_2367 0.60 +1.1 2,501,421 - Dshi_2367 0.68 -2.2 2,501,446 + Dshi_2367 0.72 +1.8 2,501,471 + Dshi_2367 0.75 -1.2 2,501,479 - Dshi_2367 0.76 +1.1 2,501,479 - Dshi_2367 0.76 +1.1 2,501,545 - Dshi_2367 0.85 +2.2 2,501,614 + -0.9 2,501,623 + +1.1 2,501,623 - -0.9 2,501,631 - +1.8 2,501,698 + Dshi_2368 0.16 +0.7 2,501,713 + Dshi_2368 0.21 +0.1 2,501,789 - Dshi_2368 0.46 -1.4 2,501,803 + Dshi_2368 0.50 +1.3
Or see this region's nucleotide sequence