Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2278 and Dshi_2279 are separated by 114 nucleotides Dshi_2279 and Dshi_2280 overlap by 31 nucleotides Dshi_2280 and Dshi_2281 are separated by 22 nucleotides
Dshi_2278: Dshi_2278 - molybdopterin oxidoreductase (RefSeq), at 2,402,247 to 2,404,919
_2278
Dshi_2279: Dshi_2279 - two component transcriptional regulator, winged helix family (RefSeq), at 2,405,034 to 2,405,738
_2279
Dshi_2280: Dshi_2280 - multi-sensor hybrid histidine kinase (RefSeq), at 2,405,708 to 2,408,494
_2280
Dshi_2281: Dshi_2281 - periplasmic binding protein/LacI transcriptional regulator (RefSeq), at 2,408,517 to 2,409,482
_2281
Position (kb)
2405
2406
2407
2408
2409 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2404.735 kb on + strand at 2404.736 kb on - strand at 2404.743 kb on - strand at 2404.772 kb on + strand at 2404.811 kb on + strand at 2404.817 kb on + strand at 2404.850 kb on + strand at 2404.858 kb on - strand at 2404.868 kb on - strand at 2404.881 kb on - strand at 2405.030 kb on + strand at 2405.030 kb on + strand at 2405.102 kb on - strand at 2405.116 kb on - strand, within Dshi_2279 at 2405.171 kb on + strand, within Dshi_2279 at 2405.185 kb on - strand, within Dshi_2279 at 2405.235 kb on - strand, within Dshi_2279 at 2405.272 kb on - strand, within Dshi_2279 at 2405.317 kb on - strand, within Dshi_2279 at 2405.409 kb on + strand, within Dshi_2279 at 2405.477 kb on + strand, within Dshi_2279 at 2405.477 kb on + strand, within Dshi_2279 at 2405.558 kb on - strand, within Dshi_2279 at 2405.564 kb on - strand, within Dshi_2279 at 2405.602 kb on - strand, within Dshi_2279 at 2405.705 kb on + strand at 2405.714 kb on - strand at 2405.714 kb on - strand at 2405.760 kb on + strand at 2405.782 kb on + strand at 2405.855 kb on - strand at 2405.872 kb on - strand at 2405.895 kb on + strand at 2405.895 kb on + strand at 2405.895 kb on + strand at 2405.904 kb on - strand at 2405.911 kb on - strand at 2405.919 kb on + strand at 2405.921 kb on - strand at 2405.926 kb on + strand at 2405.927 kb on - strand at 2405.934 kb on - strand at 2405.934 kb on - strand at 2405.997 kb on + strand, within Dshi_2280 at 2406.005 kb on - strand, within Dshi_2280 at 2406.016 kb on + strand, within Dshi_2280 at 2406.106 kb on - strand, within Dshi_2280 at 2406.154 kb on - strand, within Dshi_2280 at 2406.154 kb on - strand, within Dshi_2280 at 2406.161 kb on + strand, within Dshi_2280 at 2406.169 kb on - strand, within Dshi_2280 at 2406.169 kb on - strand, within Dshi_2280 at 2406.173 kb on + strand, within Dshi_2280 at 2406.252 kb on - strand, within Dshi_2280 at 2406.283 kb on + strand, within Dshi_2280 at 2406.299 kb on - strand, within Dshi_2280 at 2406.311 kb on + strand, within Dshi_2280 at 2406.327 kb on - strand, within Dshi_2280 at 2406.475 kb on + strand, within Dshi_2280 at 2406.475 kb on + strand, within Dshi_2280 at 2406.483 kb on - strand, within Dshi_2280 at 2406.492 kb on - strand, within Dshi_2280 at 2406.576 kb on - strand, within Dshi_2280 at 2406.592 kb on + strand, within Dshi_2280 at 2406.657 kb on - strand, within Dshi_2280 at 2406.680 kb on - strand, within Dshi_2280 at 2406.731 kb on + strand, within Dshi_2280 at 2406.732 kb on - strand, within Dshi_2280 at 2406.770 kb on + strand, within Dshi_2280 at 2406.917 kb on + strand, within Dshi_2280 at 2407.002 kb on + strand, within Dshi_2280 at 2407.065 kb on + strand, within Dshi_2280 at 2407.092 kb on + strand, within Dshi_2280 at 2407.110 kb on - strand, within Dshi_2280 at 2407.202 kb on - strand, within Dshi_2280 at 2407.218 kb on - strand, within Dshi_2280 at 2407.287 kb on + strand, within Dshi_2280 at 2407.343 kb on - strand, within Dshi_2280 at 2407.368 kb on + strand, within Dshi_2280 at 2407.376 kb on - strand, within Dshi_2280 at 2407.459 kb on + strand, within Dshi_2280 at 2407.489 kb on - strand, within Dshi_2280 at 2407.505 kb on + strand, within Dshi_2280 at 2407.540 kb on - strand, within Dshi_2280 at 2407.655 kb on - strand, within Dshi_2280 at 2407.664 kb on + strand, within Dshi_2280 at 2407.669 kb on + strand, within Dshi_2280 at 2407.740 kb on - strand, within Dshi_2280 at 2407.780 kb on - strand, within Dshi_2280 at 2407.780 kb on - strand, within Dshi_2280 at 2407.843 kb on - strand, within Dshi_2280 at 2407.885 kb on + strand, within Dshi_2280 at 2407.937 kb on + strand, within Dshi_2280 at 2407.937 kb on + strand, within Dshi_2280 at 2407.937 kb on + strand, within Dshi_2280 at 2407.944 kb on - strand, within Dshi_2280 at 2407.945 kb on - strand, within Dshi_2280 at 2407.945 kb on - strand, within Dshi_2280 at 2407.945 kb on - strand, within Dshi_2280 at 2407.945 kb on - strand, within Dshi_2280 at 2407.945 kb on - strand, within Dshi_2280 at 2407.971 kb on + strand, within Dshi_2280 at 2407.971 kb on + strand, within Dshi_2280 at 2408.037 kb on - strand, within Dshi_2280 at 2408.233 kb on + strand at 2408.252 kb on - strand at 2408.269 kb on - strand at 2408.271 kb on - strand at 2408.309 kb on - strand at 2408.314 kb on + strand at 2408.319 kb on - strand at 2408.327 kb on + strand at 2408.335 kb on - strand at 2408.341 kb on + strand at 2408.399 kb on - strand at 2408.448 kb on + strand at 2408.451 kb on + strand at 2408.451 kb on - strand at 2408.459 kb on - strand at 2408.466 kb on - strand at 2408.504 kb on + strand at 2408.512 kb on + strand at 2408.512 kb on + strand at 2408.513 kb on - strand at 2408.513 kb on - strand at 2408.527 kb on + strand at 2408.530 kb on - strand at 2408.544 kb on - strand at 2408.544 kb on - strand at 2408.555 kb on + strand at 2408.555 kb on + strand at 2408.555 kb on + strand at 2408.563 kb on - strand at 2408.563 kb on - strand at 2408.563 kb on - strand at 2408.563 kb on - strand at 2408.569 kb on + strand at 2408.586 kb on + strand at 2408.633 kb on + strand, within Dshi_2281 at 2408.635 kb on - strand, within Dshi_2281 at 2408.658 kb on - strand, within Dshi_2281 at 2408.731 kb on + strand, within Dshi_2281 at 2408.766 kb on + strand, within Dshi_2281 at 2408.774 kb on - strand, within Dshi_2281 at 2408.779 kb on + strand, within Dshi_2281 at 2408.789 kb on + strand, within Dshi_2281 at 2408.806 kb on - strand, within Dshi_2281 at 2408.812 kb on + strand, within Dshi_2281 at 2408.812 kb on + strand, within Dshi_2281 at 2408.812 kb on + strand, within Dshi_2281 at 2408.812 kb on + strand, within Dshi_2281 at 2408.812 kb on + strand, within Dshi_2281 at 2408.820 kb on - strand, within Dshi_2281 at 2408.835 kb on + strand, within Dshi_2281 at 2408.841 kb on + strand, within Dshi_2281 at 2408.860 kb on + strand, within Dshi_2281 at 2408.892 kb on + strand, within Dshi_2281 at 2408.900 kb on - strand, within Dshi_2281 at 2408.971 kb on - strand, within Dshi_2281 at 2408.996 kb on + strand, within Dshi_2281 at 2409.033 kb on - strand, within Dshi_2281 at 2409.035 kb on + strand, within Dshi_2281 at 2409.043 kb on - strand, within Dshi_2281 at 2409.045 kb on + strand, within Dshi_2281 at 2409.053 kb on - strand, within Dshi_2281 at 2409.163 kb on - strand, within Dshi_2281 at 2409.228 kb on - strand, within Dshi_2281 at 2409.238 kb on - strand, within Dshi_2281 at 2409.283 kb on + strand, within Dshi_2281 at 2409.283 kb on + strand, within Dshi_2281 at 2409.360 kb on + strand, within Dshi_2281 at 2409.394 kb on + strand at 2409.430 kb on + strand at 2409.438 kb on - strand at 2409.474 kb on + strand at 2409.476 kb on + strand at 2409.482 kb on - strand at 2409.490 kb on - strand at 2409.491 kb on + strand at 2409.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 2,404,735 + -0.3 2,404,736 - -0.9 2,404,743 - +0.6 2,404,772 + -0.5 2,404,811 + +1.1 2,404,817 + -0.1 2,404,850 + -2.7 2,404,858 - -0.7 2,404,868 - -0.7 2,404,881 - -0.2 2,405,030 + -1.0 2,405,030 + +0.1 2,405,102 - -0.0 2,405,116 - Dshi_2279 0.12 -2.9 2,405,171 + Dshi_2279 0.19 +0.2 2,405,185 - Dshi_2279 0.21 -0.3 2,405,235 - Dshi_2279 0.29 -1.7 2,405,272 - Dshi_2279 0.34 +3.5 2,405,317 - Dshi_2279 0.40 -0.4 2,405,409 + Dshi_2279 0.53 -0.6 2,405,477 + Dshi_2279 0.63 +0.0 2,405,477 + Dshi_2279 0.63 +3.1 2,405,558 - Dshi_2279 0.74 -3.4 2,405,564 - Dshi_2279 0.75 -0.9 2,405,602 - Dshi_2279 0.81 -2.1 2,405,705 + -0.1 2,405,714 - -0.8 2,405,714 - +1.1 2,405,760 + +0.8 2,405,782 + +0.3 2,405,855 - -0.6 2,405,872 - +2.1 2,405,895 + -2.6 2,405,895 + +0.9 2,405,895 + -0.3 2,405,904 - -0.5 2,405,911 - -0.2 2,405,919 + +0.2 2,405,921 - +0.1 2,405,926 + -2.4 2,405,927 - -0.3 2,405,934 - -0.6 2,405,934 - +1.4 2,405,997 + Dshi_2280 0.10 -2.1 2,406,005 - Dshi_2280 0.11 -1.6 2,406,016 + Dshi_2280 0.11 +0.2 2,406,106 - Dshi_2280 0.14 +1.1 2,406,154 - Dshi_2280 0.16 -1.9 2,406,154 - Dshi_2280 0.16 +1.2 2,406,161 + Dshi_2280 0.16 -0.4 2,406,169 - Dshi_2280 0.17 -0.3 2,406,169 - Dshi_2280 0.17 -1.4 2,406,173 + Dshi_2280 0.17 -0.3 2,406,252 - Dshi_2280 0.20 +0.6 2,406,283 + Dshi_2280 0.21 -4.2 2,406,299 - Dshi_2280 0.21 +0.3 2,406,311 + Dshi_2280 0.22 -0.4 2,406,327 - Dshi_2280 0.22 +0.0 2,406,475 + Dshi_2280 0.28 +0.1 2,406,475 + Dshi_2280 0.28 +1.1 2,406,483 - Dshi_2280 0.28 +0.9 2,406,492 - Dshi_2280 0.28 +0.4 2,406,576 - Dshi_2280 0.31 -0.2 2,406,592 + Dshi_2280 0.32 +1.3 2,406,657 - Dshi_2280 0.34 -0.7 2,406,680 - Dshi_2280 0.35 -1.1 2,406,731 + Dshi_2280 0.37 -0.9 2,406,732 - Dshi_2280 0.37 -3.4 2,406,770 + Dshi_2280 0.38 +0.3 2,406,917 + Dshi_2280 0.43 -0.7 2,407,002 + Dshi_2280 0.46 +0.5 2,407,065 + Dshi_2280 0.49 +0.7 2,407,092 + Dshi_2280 0.50 -2.5 2,407,110 - Dshi_2280 0.50 -1.2 2,407,202 - Dshi_2280 0.54 -0.8 2,407,218 - Dshi_2280 0.54 -0.1 2,407,287 + Dshi_2280 0.57 -0.2 2,407,343 - Dshi_2280 0.59 +1.5 2,407,368 + Dshi_2280 0.60 -0.3 2,407,376 - Dshi_2280 0.60 -0.2 2,407,459 + Dshi_2280 0.63 -0.6 2,407,489 - Dshi_2280 0.64 +3.7 2,407,505 + Dshi_2280 0.64 -2.4 2,407,540 - Dshi_2280 0.66 +0.2 2,407,655 - Dshi_2280 0.70 -1.9 2,407,664 + Dshi_2280 0.70 -0.1 2,407,669 + Dshi_2280 0.70 -0.6 2,407,740 - Dshi_2280 0.73 -3.0 2,407,780 - Dshi_2280 0.74 +0.8 2,407,780 - Dshi_2280 0.74 -1.1 2,407,843 - Dshi_2280 0.77 +0.4 2,407,885 + Dshi_2280 0.78 +0.0 2,407,937 + Dshi_2280 0.80 -1.3 2,407,937 + Dshi_2280 0.80 -1.6 2,407,937 + Dshi_2280 0.80 -2.7 2,407,944 - Dshi_2280 0.80 +1.5 2,407,945 - Dshi_2280 0.80 +0.6 2,407,945 - Dshi_2280 0.80 +1.1 2,407,945 - Dshi_2280 0.80 +0.8 2,407,945 - Dshi_2280 0.80 -0.2 2,407,945 - Dshi_2280 0.80 -0.4 2,407,971 + Dshi_2280 0.81 +0.3 2,407,971 + Dshi_2280 0.81 -0.3 2,408,037 - Dshi_2280 0.84 -0.7 2,408,233 + -0.3 2,408,252 - -0.5 2,408,269 - -4.6 2,408,271 - -0.2 2,408,309 - -0.2 2,408,314 + -3.3 2,408,319 - +0.4 2,408,327 + +0.2 2,408,335 - -1.2 2,408,341 + -0.8 2,408,399 - -0.5 2,408,448 + -1.0 2,408,451 + -2.7 2,408,451 - +0.3 2,408,459 - -1.5 2,408,466 - -0.5 2,408,504 + -1.1 2,408,512 + +0.2 2,408,512 + -0.5 2,408,513 - -1.0 2,408,513 - +0.1 2,408,527 + +1.5 2,408,530 - -0.6 2,408,544 - -1.9 2,408,544 - -2.5 2,408,555 + +1.5 2,408,555 + +0.7 2,408,555 + +0.5 2,408,563 - +0.1 2,408,563 - -0.5 2,408,563 - +0.1 2,408,563 - -0.2 2,408,569 + -0.8 2,408,586 + -0.2 2,408,633 + Dshi_2281 0.12 -1.2 2,408,635 - Dshi_2281 0.12 +2.7 2,408,658 - Dshi_2281 0.15 -0.7 2,408,731 + Dshi_2281 0.22 -2.1 2,408,766 + Dshi_2281 0.26 -0.6 2,408,774 - Dshi_2281 0.27 +1.5 2,408,779 + Dshi_2281 0.27 -0.3 2,408,789 + Dshi_2281 0.28 -1.1 2,408,806 - Dshi_2281 0.30 +0.6 2,408,812 + Dshi_2281 0.31 +1.2 2,408,812 + Dshi_2281 0.31 -0.8 2,408,812 + Dshi_2281 0.31 -2.5 2,408,812 + Dshi_2281 0.31 +0.4 2,408,812 + Dshi_2281 0.31 +0.4 2,408,820 - Dshi_2281 0.31 -1.1 2,408,835 + Dshi_2281 0.33 +0.5 2,408,841 + Dshi_2281 0.34 +0.2 2,408,860 + Dshi_2281 0.36 -0.1 2,408,892 + Dshi_2281 0.39 +0.7 2,408,900 - Dshi_2281 0.40 -1.0 2,408,971 - Dshi_2281 0.47 -2.2 2,408,996 + Dshi_2281 0.50 -1.2 2,409,033 - Dshi_2281 0.53 +3.2 2,409,035 + Dshi_2281 0.54 -4.0 2,409,043 - Dshi_2281 0.54 -0.7 2,409,045 + Dshi_2281 0.55 -1.8 2,409,053 - Dshi_2281 0.55 -0.2 2,409,163 - Dshi_2281 0.67 +1.5 2,409,228 - Dshi_2281 0.74 -0.5 2,409,238 - Dshi_2281 0.75 -0.9 2,409,283 + Dshi_2281 0.79 -0.8 2,409,283 + Dshi_2281 0.79 -1.8 2,409,360 + Dshi_2281 0.87 +3.9 2,409,394 + +0.7 2,409,430 + +0.6 2,409,438 - -1.9 2,409,474 + -0.8 2,409,476 + -3.0 2,409,482 - +0.7 2,409,490 - +1.9 2,409,491 + -0.5 2,409,491 + -0.9
Or see this region's nucleotide sequence