Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1753 and Dshi_1754 are separated by 90 nucleotides Dshi_1754 and Dshi_1755 overlap by 8 nucleotides Dshi_1755 and Dshi_1756 overlap by 11 nucleotides Dshi_1756 and Dshi_1757 overlap by 10 nucleotides Dshi_1757 and Dshi_1758 are separated by 109 nucleotides
Dshi_1753: Dshi_1753 - Enoyl-CoA hydratase/isomerase (RefSeq), at 1,819,173 to 1,819,949
_1753
Dshi_1754: Dshi_1754 - hypothetical protein (RefSeq), at 1,820,040 to 1,820,456
_1754
Dshi_1755: Dshi_1755 - hypothetical protein (RefSeq), at 1,820,449 to 1,820,655
_1755
Dshi_1756: Dshi_1756 - hypothetical protein (RefSeq), at 1,820,645 to 1,821,166
_1756
Dshi_1757: Dshi_1757 - protein of unknown function DUF847 (RefSeq), at 1,821,157 to 1,821,768
_1757
Dshi_1758: Dshi_1758 - CcmE/CycJ protein (RefSeq), at 1,821,878 to 1,822,360
_1758
Position (kb)
1820
1821
1822 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1819.656 kb on + strand, within Dshi_1753 at 1819.766 kb on - strand, within Dshi_1753 at 1819.811 kb on - strand, within Dshi_1753 at 1819.936 kb on - strand at 1819.945 kb on + strand at 1819.953 kb on - strand at 1820.011 kb on - strand at 1820.188 kb on + strand, within Dshi_1754 at 1820.196 kb on - strand, within Dshi_1754 at 1820.202 kb on - strand, within Dshi_1754 at 1820.238 kb on - strand, within Dshi_1754 at 1820.366 kb on - strand, within Dshi_1754 at 1820.412 kb on + strand, within Dshi_1754 at 1820.420 kb on - strand at 1820.482 kb on + strand, within Dshi_1755 at 1820.515 kb on + strand, within Dshi_1755 at 1820.549 kb on - strand, within Dshi_1755 at 1820.892 kb on - strand, within Dshi_1756 at 1820.903 kb on + strand, within Dshi_1756 at 1820.970 kb on - strand, within Dshi_1756 at 1821.036 kb on - strand, within Dshi_1756 at 1821.102 kb on - strand, within Dshi_1756 at 1821.105 kb on + strand, within Dshi_1756 at 1821.215 kb on + strand at 1821.352 kb on - strand, within Dshi_1757 at 1821.417 kb on + strand, within Dshi_1757 at 1821.512 kb on - strand, within Dshi_1757 at 1821.519 kb on + strand, within Dshi_1757 at 1821.539 kb on - strand, within Dshi_1757 at 1821.759 kb on - strand at 1821.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 1,819,656 + Dshi_1753 0.62 +0.4 1,819,766 - Dshi_1753 0.76 +1.1 1,819,811 - Dshi_1753 0.82 +4.0 1,819,936 - +2.0 1,819,945 + -1.3 1,819,953 - +0.9 1,820,011 - -1.7 1,820,188 + Dshi_1754 0.35 +0.9 1,820,196 - Dshi_1754 0.37 +1.0 1,820,202 - Dshi_1754 0.39 -0.6 1,820,238 - Dshi_1754 0.47 +0.8 1,820,366 - Dshi_1754 0.78 -0.8 1,820,412 + Dshi_1754 0.89 -1.3 1,820,420 - +3.8 1,820,482 + Dshi_1755 0.16 +1.4 1,820,515 + Dshi_1755 0.32 +2.0 1,820,549 - Dshi_1755 0.48 +1.0 1,820,892 - Dshi_1756 0.47 +1.7 1,820,903 + Dshi_1756 0.49 +1.4 1,820,970 - Dshi_1756 0.62 +0.1 1,821,036 - Dshi_1756 0.75 +2.0 1,821,102 - Dshi_1756 0.88 -0.2 1,821,105 + Dshi_1756 0.88 -1.0 1,821,215 + +0.2 1,821,352 - Dshi_1757 0.32 +1.0 1,821,417 + Dshi_1757 0.42 -1.3 1,821,512 - Dshi_1757 0.58 +0.5 1,821,519 + Dshi_1757 0.59 -0.5 1,821,539 - Dshi_1757 0.62 +2.0 1,821,759 - -1.5 1,821,785 - +1.0
Or see this region's nucleotide sequence