Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1687 and Dshi_1688 are separated by 189 nucleotides Dshi_1688 and Dshi_1689 overlap by 4 nucleotides Dshi_1689 and Dshi_1690 overlap by 19 nucleotides Dshi_1690 and Dshi_1691 overlap by 4 nucleotides
Dshi_1687: Dshi_1687 - HNH endonuclease (RefSeq), at 1,756,672 to 1,757,268
_1687
Dshi_1688: Dshi_1688 - phospholipase/Carboxylesterase (RefSeq), at 1,757,458 to 1,758,129
_1688
Dshi_1689: Dshi_1689 - HhH-GPD family protein (RefSeq), at 1,758,126 to 1,758,758
_1689
Dshi_1690: Dshi_1690 - precorrin-6x reductase (RefSeq), at 1,758,740 to 1,759,486
_1690
Dshi_1691: Dshi_1691 - cobalamin (vitamin B12) biosynthesis CbiD protein (RefSeq), at 1,759,483 to 1,760,532
_1691
Position (kb)
1758
1759 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1757.129 kb on - strand, within Dshi_1687 at 1757.360 kb on + strand at 1758.111 kb on + strand at 1758.227 kb on + strand, within Dshi_1689 at 1758.342 kb on - strand, within Dshi_1689 at 1758.363 kb on + strand, within Dshi_1689 at 1758.403 kb on - strand, within Dshi_1689 at 1758.487 kb on - strand, within Dshi_1689 at 1758.489 kb on + strand, within Dshi_1689 at 1758.532 kb on + strand, within Dshi_1689 at 1758.625 kb on - strand, within Dshi_1689 at 1758.625 kb on - strand, within Dshi_1689 at 1758.696 kb on + strand at 1758.731 kb on - strand at 1758.773 kb on - strand at 1758.776 kb on + strand at 1758.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 1,757,129 - Dshi_1687 0.77 -1.6 1,757,360 + +1.5 1,758,111 + +1.0 1,758,227 + Dshi_1689 0.16 +1.2 1,758,342 - Dshi_1689 0.34 -0.4 1,758,363 + Dshi_1689 0.37 +0.4 1,758,403 - Dshi_1689 0.44 -1.6 1,758,487 - Dshi_1689 0.57 +0.1 1,758,489 + Dshi_1689 0.57 +4.0 1,758,532 + Dshi_1689 0.64 +1.2 1,758,625 - Dshi_1689 0.79 -0.4 1,758,625 - Dshi_1689 0.79 +1.5 1,758,696 + +1.6 1,758,731 - +1.2 1,758,773 - -1.6 1,758,776 + +3.0 1,758,776 + -0.3
Or see this region's nucleotide sequence