Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1578 and Dshi_1579 overlap by 4 nucleotides Dshi_1579 and Dshi_1580 overlap by 22 nucleotides Dshi_1580 and Dshi_1581 are separated by 23 nucleotides Dshi_1581 and Dshi_1582 are separated by 197 nucleotides Dshi_1582 and Dshi_1583 are separated by 176 nucleotides
Dshi_1578: Dshi_1578 - phosphatidylglycerophosphatase A (RefSeq), at 1,645,739 to 1,646,227
_1578
Dshi_1579: Dshi_1579 - CinA domain protein (RefSeq), at 1,646,224 to 1,646,706
_1579
Dshi_1580: Dshi_1580 - cyclase/dehydrase (RefSeq), at 1,646,685 to 1,647,200
_1580
Dshi_1581: Dshi_1581 - hypoxanthine phosphoribosyltransferase (RefSeq), at 1,647,224 to 1,647,760
_1581
Dshi_1582: Dshi_1582 - hypothetical protein (RefSeq), at 1,647,958 to 1,648,476
_1582
Dshi_1583: Dshi_1583 - lipoic acid synthetase (RefSeq), at 1,648,653 to 1,649,603
_1583
Position (kb)
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1646.264 kb on + strand at 1646.416 kb on - strand, within Dshi_1579 at 1646.422 kb on - strand, within Dshi_1579 at 1646.683 kb on + strand at 1646.693 kb on + strand at 1646.900 kb on - strand, within Dshi_1580 at 1647.063 kb on - strand, within Dshi_1580 at 1647.076 kb on + strand, within Dshi_1580 at 1647.084 kb on - strand, within Dshi_1580 at 1647.093 kb on + strand, within Dshi_1580 at 1647.094 kb on - strand, within Dshi_1580 at 1647.103 kb on + strand, within Dshi_1580 at 1647.114 kb on - strand, within Dshi_1580 at 1647.276 kb on - strand at 1647.341 kb on - strand, within Dshi_1581 at 1647.384 kb on + strand, within Dshi_1581 at 1647.427 kb on + strand, within Dshi_1581 at 1647.548 kb on + strand, within Dshi_1581 at 1647.561 kb on - strand, within Dshi_1581 at 1647.579 kb on + strand, within Dshi_1581 at 1647.621 kb on + strand, within Dshi_1581 at 1647.754 kb on - strand at 1647.873 kb on + strand at 1647.963 kb on - strand at 1647.974 kb on + strand at 1648.071 kb on + strand, within Dshi_1582 at 1648.076 kb on + strand, within Dshi_1582 at 1648.081 kb on + strand, within Dshi_1582 at 1648.084 kb on - strand, within Dshi_1582 at 1648.104 kb on + strand, within Dshi_1582 at 1648.369 kb on - strand, within Dshi_1582 at 1648.414 kb on - strand, within Dshi_1582 at 1648.419 kb on - strand, within Dshi_1582 at 1648.516 kb on - strand at 1648.592 kb on + strand at 1648.592 kb on + strand at 1648.600 kb on - strand at 1648.600 kb on - strand at 1648.600 kb on - strand at 1648.600 kb on - strand at 1648.600 kb on - strand at 1648.607 kb on + strand at 1648.618 kb on + strand at 1648.626 kb on - strand at 1648.626 kb on - strand at 1648.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 1,646,264 + +3.4 1,646,416 - Dshi_1579 0.40 +2.6 1,646,422 - Dshi_1579 0.41 -1.1 1,646,683 + +1.6 1,646,693 + -2.4 1,646,900 - Dshi_1580 0.42 -0.1 1,647,063 - Dshi_1580 0.73 +0.0 1,647,076 + Dshi_1580 0.76 -2.0 1,647,084 - Dshi_1580 0.77 -0.5 1,647,093 + Dshi_1580 0.79 -0.5 1,647,094 - Dshi_1580 0.79 +0.0 1,647,103 + Dshi_1580 0.81 -1.8 1,647,114 - Dshi_1580 0.83 -1.0 1,647,276 - -2.8 1,647,341 - Dshi_1581 0.22 -0.7 1,647,384 + Dshi_1581 0.30 +1.4 1,647,427 + Dshi_1581 0.38 -1.3 1,647,548 + Dshi_1581 0.60 +0.1 1,647,561 - Dshi_1581 0.63 +4.0 1,647,579 + Dshi_1581 0.66 +3.7 1,647,621 + Dshi_1581 0.74 -0.8 1,647,754 - +0.5 1,647,873 + +0.1 1,647,963 - -0.5 1,647,974 + -1.5 1,648,071 + Dshi_1582 0.22 -1.4 1,648,076 + Dshi_1582 0.23 -1.5 1,648,081 + Dshi_1582 0.24 +0.3 1,648,084 - Dshi_1582 0.24 +4.6 1,648,104 + Dshi_1582 0.28 -1.9 1,648,369 - Dshi_1582 0.79 +0.4 1,648,414 - Dshi_1582 0.88 -0.7 1,648,419 - Dshi_1582 0.89 +0.8 1,648,516 - -2.5 1,648,592 + +0.3 1,648,592 + -0.2 1,648,600 - +4.5 1,648,600 - -1.8 1,648,600 - +1.0 1,648,600 - -0.3 1,648,600 - -3.4 1,648,607 + +0.0 1,648,618 + +1.0 1,648,626 - -1.2 1,648,626 - -2.2 1,648,626 - +1.3
Or see this region's nucleotide sequence