Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1068 and Dshi_1069 are separated by 61 nucleotides Dshi_1069 and Dshi_1070 are separated by 187 nucleotides Dshi_1070 and Dshi_1071 overlap by 4 nucleotides Dshi_1071 and Dshi_1072 overlap by 20 nucleotides Dshi_1072 and Dshi_1073 are separated by 58 nucleotides
Dshi_1068: Dshi_1068 - hypothetical protein (RefSeq), at 1,099,844 to 1,100,293
_1068
Dshi_1069: Dshi_1069 - Fe-S metabolism associated SufE (RefSeq), at 1,100,355 to 1,100,849
_1069
Dshi_1070: Dshi_1070 - hypothetical protein (RefSeq), at 1,101,037 to 1,101,669
_1070
Dshi_1071: Dshi_1071 - protein of unknown function DUF74 (RefSeq), at 1,101,666 to 1,102,001
_1071
Dshi_1072: Dshi_1072 - hypothetical protein (RefSeq), at 1,101,982 to 1,102,200
_1072
Dshi_1073: Dshi_1073 - Methionine synthase (RefSeq), at 1,102,259 to 1,103,038
_1073
Position (kb)
1101
1102 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1100.058 kb on - strand, within Dshi_1068 at 1100.181 kb on + strand, within Dshi_1068 at 1100.189 kb on - strand, within Dshi_1068 at 1100.366 kb on + strand at 1100.399 kb on - strand at 1101.688 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 1,100,058 - Dshi_1068 0.48 +3.8 1,100,181 + Dshi_1068 0.75 +1.6 1,100,189 - Dshi_1068 0.77 -0.0 1,100,366 + -0.0 1,100,399 - -0.4 1,101,688 + -0.0
Or see this region's nucleotide sequence