Experiment: Anaero. nitrate (e.a.), marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0065 and Dshi_0066 are separated by 115 nucleotides Dshi_0066 and Dshi_0067 overlap by 4 nucleotides Dshi_0067 and Dshi_0068 are separated by 4 nucleotides
Dshi_0065: Dshi_0065 - hypothetical protein (RefSeq), at 65,978 to 67,468
_0065
Dshi_0066: Dshi_0066 - short-chain dehydrogenase/reductase SDR (RefSeq), at 67,584 to 68,342
_0066
Dshi_0067: Dshi_0067 - Enoyl-CoA hydratase/isomerase (RefSeq), at 68,339 to 69,085
_0067
Dshi_0068: Dshi_0068 - lipoprotein, putative (RefSeq), at 69,090 to 69,674
_0068
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 66.673 kb on - strand, within Dshi_0065 at 66.817 kb on - strand, within Dshi_0065 at 66.990 kb on - strand, within Dshi_0065 at 67.067 kb on + strand, within Dshi_0065 at 67.077 kb on + strand, within Dshi_0065 at 67.080 kb on - strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.104 kb on + strand, within Dshi_0065 at 67.126 kb on + strand at 67.135 kb on - strand, within Dshi_0065 at 67.242 kb on - strand, within Dshi_0065 at 67.365 kb on + strand at 67.366 kb on + strand at 67.373 kb on - strand at 67.395 kb on - strand at 67.406 kb on - strand at 67.417 kb on - strand at 67.456 kb on + strand at 67.474 kb on - strand at 67.527 kb on - strand at 67.531 kb on + strand at 67.531 kb on + strand at 67.537 kb on - strand at 67.553 kb on - strand at 67.563 kb on + strand at 67.578 kb on - strand at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.810 kb on - strand at 67.810 kb on - strand, within Dshi_0066 at 67.851 kb on - strand, within Dshi_0066 at 67.920 kb on - strand, within Dshi_0066 at 67.931 kb on + strand, within Dshi_0066 at 68.208 kb on + strand, within Dshi_0066 at 68.251 kb on - strand, within Dshi_0066 at 68.393 kb on + strand at 68.398 kb on + strand at 68.533 kb on + strand, within Dshi_0067 at 68.585 kb on + strand, within Dshi_0067 at 68.594 kb on - strand, within Dshi_0067 at 68.595 kb on + strand, within Dshi_0067 at 68.600 kb on + strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.772 kb on - strand, within Dshi_0067 at 68.935 kb on + strand, within Dshi_0067
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. nitrate (e.a.), marine broth remove 66,673 - Dshi_0065 0.47 -0.0 66,817 - Dshi_0065 0.56 +0.5 66,990 - Dshi_0065 0.68 +2.1 67,067 + Dshi_0065 0.73 -1.0 67,077 + Dshi_0065 0.74 +1.6 67,080 - Dshi_0065 0.74 +1.0 67,104 + Dshi_0065 0.76 +0.2 67,104 + Dshi_0065 0.76 +1.0 67,126 + -0.6 67,135 - Dshi_0065 0.78 +2.6 67,242 - Dshi_0065 0.85 -2.0 67,365 + +0.1 67,366 + -0.0 67,373 - +3.4 67,395 - +1.0 67,406 - +1.0 67,417 - -1.1 67,456 + +0.1 67,474 - -2.9 67,527 - +0.8 67,531 + -1.8 67,531 + +2.6 67,537 - +1.6 67,553 - +1.0 67,563 + -3.0 67,578 - -3.5 67,748 + Dshi_0066 0.22 -1.1 67,748 + Dshi_0066 0.22 -0.6 67,748 + Dshi_0066 0.22 +0.4 67,810 - +1.0 67,810 - Dshi_0066 0.30 -1.6 67,851 - Dshi_0066 0.35 +3.4 67,920 - Dshi_0066 0.44 +0.5 67,931 + Dshi_0066 0.46 +1.7 68,208 + Dshi_0066 0.82 -2.2 68,251 - Dshi_0066 0.88 +4.0 68,393 + +0.1 68,398 + -0.0 68,533 + Dshi_0067 0.26 +2.1 68,585 + Dshi_0067 0.33 -0.6 68,594 - Dshi_0067 0.34 +2.6 68,595 + Dshi_0067 0.34 -1.2 68,600 + Dshi_0067 0.35 +2.6 68,603 - Dshi_0067 0.35 +1.8 68,603 - Dshi_0067 0.35 +2.6 68,772 - Dshi_0067 0.58 +1.4 68,935 + Dshi_0067 0.80 -1.1
Or see this region's nucleotide sequence