Strain Fitness in Shewanella amazonensis SB2B around Sama_0924

Experiment: L-Lysine 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSama_0923 and ileS are separated by 42 nucleotidesileS and lspA are separated by 8 nucleotideslspA and Sama_0926 are separated by 3 nucleotidesSama_0926 and ispH overlap by 1 nucleotides Sama_0923: Sama_0923 - riboflavin biosynthesis protein RibF (RefSeq), at 1,115,436 to 1,116,371 _0923 Sama_0924: ileS - isoleucyl-tRNA synthetase (RefSeq), at 1,116,414 to 1,119,236 ileS Sama_0925: lspA - lipoprotein signal peptidase (RefSeq), at 1,119,245 to 1,119,751 lspA Sama_0926: Sama_0926 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 1,119,755 to 1,120,174 _0926 Sama_0927: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (RefSeq), at 1,120,174 to 1,121,115 ispH Position (kb) 1116 1117 1118 1119 1120Strain fitness (log2 ratio) -2 -1 0 1at 1115.416 kb on + strandat 1119.697 kb on + strand, within lspAat 1119.697 kb on + strand, within lspAat 1119.697 kb on + strand, within lspAat 1119.700 kb on + strand, within lspAat 1119.712 kb on + strandat 1119.712 kb on + strandat 1119.749 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 200 mM
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1,115,416 + -0.7
1,119,697 + lspA Sama_0925 0.89 -2.5
1,119,697 + lspA Sama_0925 0.89 -2.6
1,119,697 + lspA Sama_0925 0.89 -0.3
1,119,700 + lspA Sama_0925 0.90 -0.7
1,119,712 + -2.4
1,119,712 + -2.0
1,119,749 + -0.6

Or see this region's nucleotide sequence