Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2187 and Sama_2188 are separated by 8 nucleotides Sama_2188 and Sama_2189 are separated by 77 nucleotides Sama_2189 and secF are separated by 200 nucleotides secF and secD are separated by 13 nucleotides
Sama_2187: Sama_2187 - precorrin-2 oxidase / ferrochelatase (RefSeq), at 2,646,275 to 2,647,186
_2187
Sama_2188: Sama_2188 - rhodanese-like protein (RefSeq), at 2,647,195 to 2,647,554
_2188
Sama_2189: Sama_2189 - hypothetical protein (RefSeq), at 2,647,632 to 2,647,949
_2189
Sama_2190: secF - preprotein translocase subunit SecF (RefSeq), at 2,648,150 to 2,649,097
secF
Sama_2191: secD - preprotein translocase subunit SecD (RefSeq), at 2,649,111 to 2,650,961
secD
Position (kb)
2648
2649
2650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2647.238 kb on + strand, within Sama_2188 at 2647.239 kb on - strand, within Sama_2188 at 2647.239 kb on - strand, within Sama_2188 at 2647.239 kb on - strand, within Sama_2188 at 2647.268 kb on + strand, within Sama_2188 at 2647.288 kb on - strand, within Sama_2188 at 2647.289 kb on + strand, within Sama_2188 at 2647.290 kb on - strand, within Sama_2188 at 2647.310 kb on + strand, within Sama_2188 at 2647.325 kb on + strand, within Sama_2188 at 2647.326 kb on - strand, within Sama_2188 at 2647.479 kb on - strand, within Sama_2188 at 2647.513 kb on + strand, within Sama_2188 at 2647.546 kb on - strand at 2647.546 kb on - strand at 2647.566 kb on + strand at 2647.567 kb on - strand at 2647.590 kb on + strand at 2647.590 kb on + strand at 2647.590 kb on + strand at 2647.591 kb on - strand at 2647.591 kb on - strand at 2647.591 kb on - strand at 2647.591 kb on - strand at 2647.591 kb on - strand at 2647.596 kb on + strand at 2647.597 kb on - strand at 2647.828 kb on - strand, within Sama_2189 at 2647.869 kb on - strand, within Sama_2189 at 2647.869 kb on - strand, within Sama_2189 at 2647.937 kb on + strand at 2647.938 kb on - strand at 2647.961 kb on - strand at 2647.961 kb on - strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.966 kb on + strand at 2647.967 kb on - strand at 2647.967 kb on - strand at 2647.967 kb on - strand at 2647.967 kb on - strand at 2647.967 kb on - strand at 2647.984 kb on + strand at 2647.984 kb on + strand at 2647.984 kb on + strand at 2647.985 kb on - strand at 2647.985 kb on - strand at 2647.985 kb on - strand at 2647.986 kb on + strand at 2647.986 kb on + strand at 2647.986 kb on + strand at 2647.986 kb on + strand at 2647.986 kb on + strand at 2647.987 kb on - strand at 2647.987 kb on - strand at 2648.015 kb on - strand at 2648.087 kb on - strand at 2648.134 kb on - strand at 2648.143 kb on - strand at 2648.143 kb on - strand at 2648.151 kb on + strand at 2648.151 kb on + strand at 2648.152 kb on - strand at 2648.152 kb on - strand at 2648.254 kb on - strand, within secF at 2648.254 kb on - strand, within secF at 2648.266 kb on - strand, within secF at 2648.266 kb on - strand, within secF at 2648.381 kb on - strand, within secF at 2648.480 kb on - strand, within secF at 2648.480 kb on - strand, within secF at 2648.644 kb on - strand, within secF at 2649.138 kb on - strand at 2649.386 kb on - strand, within secD at 2649.545 kb on - strand, within secD at 2649.548 kb on - strand, within secD at 2649.548 kb on - strand, within secD at 2649.780 kb on - strand, within secD at 2649.780 kb on - strand, within secD at 2649.822 kb on - strand, within secD at 2649.842 kb on - strand, within secD at 2649.842 kb on - strand, within secD at 2649.842 kb on - strand, within secD
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 2,647,238 + Sama_2188 0.12 +1.6 2,647,239 - Sama_2188 0.12 +3.5 2,647,239 - Sama_2188 0.12 +0.2 2,647,239 - Sama_2188 0.12 -0.4 2,647,268 + Sama_2188 0.20 +1.1 2,647,288 - Sama_2188 0.26 -1.4 2,647,289 + Sama_2188 0.26 -0.9 2,647,290 - Sama_2188 0.26 +1.6 2,647,310 + Sama_2188 0.32 -1.9 2,647,325 + Sama_2188 0.36 -2.5 2,647,326 - Sama_2188 0.36 +1.2 2,647,479 - Sama_2188 0.79 +0.2 2,647,513 + Sama_2188 0.88 +0.6 2,647,546 - -0.9 2,647,546 - +0.6 2,647,566 + -1.9 2,647,567 - -0.4 2,647,590 + -0.8 2,647,590 + +0.1 2,647,590 + +0.6 2,647,591 - -0.4 2,647,591 - +1.1 2,647,591 - +0.6 2,647,591 - -1.1 2,647,591 - -0.7 2,647,596 + +0.1 2,647,597 - -0.9 2,647,828 - Sama_2189 0.62 -1.7 2,647,869 - Sama_2189 0.75 +1.2 2,647,869 - Sama_2189 0.75 -0.7 2,647,937 + -1.7 2,647,938 - +1.2 2,647,961 - -0.8 2,647,961 - +0.6 2,647,966 + +0.2 2,647,966 + -0.2 2,647,966 + +0.1 2,647,966 + +0.3 2,647,966 + +0.6 2,647,966 + -0.1 2,647,966 + -0.1 2,647,967 - +0.3 2,647,967 - +0.6 2,647,967 - +0.8 2,647,967 - -0.2 2,647,967 - -0.8 2,647,984 + -0.4 2,647,984 + -0.1 2,647,984 + +0.9 2,647,985 - +1.5 2,647,985 - +1.1 2,647,985 - +0.4 2,647,986 + -0.0 2,647,986 + -0.9 2,647,986 + -0.6 2,647,986 + +0.6 2,647,986 + -0.7 2,647,987 - -2.2 2,647,987 - -0.4 2,648,015 - -0.9 2,648,087 - +1.2 2,648,134 - +0.6 2,648,143 - -0.4 2,648,143 - -0.6 2,648,151 + -0.7 2,648,151 + -1.2 2,648,152 - +0.1 2,648,152 - +0.1 2,648,254 - secF Sama_2190 0.11 +0.1 2,648,254 - secF Sama_2190 0.11 -0.4 2,648,266 - secF Sama_2190 0.12 -2.2 2,648,266 - secF Sama_2190 0.12 -0.9 2,648,381 - secF Sama_2190 0.24 -0.4 2,648,480 - secF Sama_2190 0.35 +0.6 2,648,480 - secF Sama_2190 0.35 -0.4 2,648,644 - secF Sama_2190 0.52 -0.4 2,649,138 - -0.9 2,649,386 - secD Sama_2191 0.15 -0.9 2,649,545 - secD Sama_2191 0.23 -1.7 2,649,548 - secD Sama_2191 0.24 -0.9 2,649,548 - secD Sama_2191 0.24 -0.9 2,649,780 - secD Sama_2191 0.36 -1.7 2,649,780 - secD Sama_2191 0.36 -0.4 2,649,822 - secD Sama_2191 0.38 -1.4 2,649,842 - secD Sama_2191 0.39 -2.8 2,649,842 - secD Sama_2191 0.39 +0.6 2,649,842 - secD Sama_2191 0.39 -0.4
Or see this region's nucleotide sequence