Experiment: m.b. 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_5008 and Dshi_2113 are separated by 4 nucleotides Dshi_2113 and Dshi_2114 are separated by 82 nucleotides Dshi_2114 and Dshi_2115 are separated by 13 nucleotides
Dshi_5008: Dshi_5008 - L-serine dehydratase (NCBI), at 2,240,003 to 2,241,415
_5008
Dshi_2113: Dshi_2113 - Glycine hydroxymethyltransferase (RefSeq), at 2,241,420 to 2,242,694
_2113
Dshi_2114: Dshi_2114 - Sarcosine oxidase gamma subunit (RefSeq), at 2,242,777 to 2,243,307
_2114
Dshi_2115: Dshi_2115 - sarcosine oxidase, alpha subunit family (RefSeq), at 2,243,321 to 2,246,323
_2115
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2240.427 kb on - strand, within Dshi_5008 at 2240.444 kb on - strand, within Dshi_5008 at 2240.573 kb on - strand, within Dshi_5008 at 2240.606 kb on - strand, within Dshi_5008 at 2240.725 kb on + strand, within Dshi_5008 at 2240.735 kb on + strand, within Dshi_5008 at 2240.753 kb on + strand, within Dshi_5008 at 2240.768 kb on + strand, within Dshi_5008 at 2240.812 kb on + strand, within Dshi_5008 at 2240.860 kb on + strand, within Dshi_5008 at 2240.860 kb on + strand, within Dshi_5008 at 2240.868 kb on - strand, within Dshi_5008 at 2240.899 kb on + strand, within Dshi_5008 at 2240.952 kb on - strand, within Dshi_5008 at 2240.972 kb on + strand, within Dshi_5008 at 2240.972 kb on - strand, within Dshi_5008 at 2240.998 kb on + strand, within Dshi_5008 at 2241.009 kb on - strand, within Dshi_5008 at 2241.102 kb on - strand, within Dshi_5008 at 2241.163 kb on + strand, within Dshi_5008 at 2241.253 kb on + strand, within Dshi_5008 at 2241.256 kb on - strand, within Dshi_5008 at 2241.349 kb on + strand at 2241.349 kb on + strand at 2241.376 kb on - strand at 2241.534 kb on + strand at 2241.569 kb on - strand, within Dshi_2113 at 2241.609 kb on - strand, within Dshi_2113 at 2241.614 kb on + strand, within Dshi_2113 at 2241.614 kb on + strand, within Dshi_2113 at 2241.622 kb on - strand, within Dshi_2113 at 2241.622 kb on - strand, within Dshi_2113 at 2241.917 kb on + strand, within Dshi_2113 at 2241.946 kb on + strand, within Dshi_2113 at 2242.033 kb on + strand, within Dshi_2113 at 2242.073 kb on + strand, within Dshi_2113 at 2242.187 kb on + strand, within Dshi_2113 at 2242.192 kb on + strand, within Dshi_2113 at 2242.200 kb on - strand, within Dshi_2113 at 2242.254 kb on - strand, within Dshi_2113 at 2242.264 kb on - strand, within Dshi_2113 at 2242.264 kb on - strand, within Dshi_2113 at 2242.300 kb on + strand, within Dshi_2113 at 2242.342 kb on + strand, within Dshi_2113 at 2242.342 kb on - strand, within Dshi_2113 at 2242.475 kb on - strand, within Dshi_2113 at 2242.475 kb on - strand, within Dshi_2113 at 2242.495 kb on - strand, within Dshi_2113 at 2242.725 kb on - strand at 2242.776 kb on + strand at 2242.820 kb on - strand at 2243.028 kb on + strand, within Dshi_2114 at 2243.028 kb on + strand, within Dshi_2114 at 2243.083 kb on + strand, within Dshi_2114 at 2243.137 kb on - strand, within Dshi_2114 at 2243.139 kb on - strand, within Dshi_2114 at 2243.139 kb on - strand, within Dshi_2114 at 2243.185 kb on + strand, within Dshi_2114 at 2243.193 kb on - strand, within Dshi_2114 at 2243.214 kb on - strand, within Dshi_2114 at 2243.309 kb on + strand at 2243.330 kb on - strand at 2243.431 kb on + strand at 2243.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. 30C remove 2,240,427 - Dshi_5008 0.30 -1.9 2,240,444 - Dshi_5008 0.31 -0.9 2,240,573 - Dshi_5008 0.40 -0.4 2,240,606 - Dshi_5008 0.43 -0.4 2,240,725 + Dshi_5008 0.51 -0.9 2,240,735 + Dshi_5008 0.52 +0.1 2,240,753 + Dshi_5008 0.53 +0.1 2,240,768 + Dshi_5008 0.54 -0.1 2,240,812 + Dshi_5008 0.57 +0.7 2,240,860 + Dshi_5008 0.61 -1.3 2,240,860 + Dshi_5008 0.61 +1.6 2,240,868 - Dshi_5008 0.61 -1.4 2,240,899 + Dshi_5008 0.63 -1.1 2,240,952 - Dshi_5008 0.67 -1.4 2,240,972 + Dshi_5008 0.69 +2.8 2,240,972 - Dshi_5008 0.69 -1.6 2,240,998 + Dshi_5008 0.70 +0.1 2,241,009 - Dshi_5008 0.71 -1.5 2,241,102 - Dshi_5008 0.78 -0.1 2,241,163 + Dshi_5008 0.82 -0.8 2,241,253 + Dshi_5008 0.88 +1.6 2,241,256 - Dshi_5008 0.89 +1.6 2,241,349 + -0.3 2,241,349 + +1.1 2,241,376 - -0.4 2,241,534 + +0.5 2,241,569 - Dshi_2113 0.12 -0.4 2,241,609 - Dshi_2113 0.15 -0.5 2,241,614 + Dshi_2113 0.15 -2.0 2,241,614 + Dshi_2113 0.15 +1.3 2,241,622 - Dshi_2113 0.16 +0.1 2,241,622 - Dshi_2113 0.16 -0.7 2,241,917 + Dshi_2113 0.39 -1.4 2,241,946 + Dshi_2113 0.41 -0.6 2,242,033 + Dshi_2113 0.48 +1.9 2,242,073 + Dshi_2113 0.51 -1.2 2,242,187 + Dshi_2113 0.60 -0.7 2,242,192 + Dshi_2113 0.61 -0.4 2,242,200 - Dshi_2113 0.61 -0.3 2,242,254 - Dshi_2113 0.65 -0.7 2,242,264 - Dshi_2113 0.66 +0.8 2,242,264 - Dshi_2113 0.66 +2.4 2,242,300 + Dshi_2113 0.69 +0.5 2,242,342 + Dshi_2113 0.72 -0.8 2,242,342 - Dshi_2113 0.72 -1.5 2,242,475 - Dshi_2113 0.83 +2.1 2,242,475 - Dshi_2113 0.83 +0.1 2,242,495 - Dshi_2113 0.84 +2.9 2,242,725 - -0.5 2,242,776 + -0.5 2,242,820 - +0.1 2,243,028 + Dshi_2114 0.47 -1.1 2,243,028 + Dshi_2114 0.47 -0.3 2,243,083 + Dshi_2114 0.58 -0.5 2,243,137 - Dshi_2114 0.68 +0.5 2,243,139 - Dshi_2114 0.68 -0.7 2,243,139 - Dshi_2114 0.68 +2.4 2,243,185 + Dshi_2114 0.77 +0.8 2,243,193 - Dshi_2114 0.78 +0.2 2,243,214 - Dshi_2114 0.82 -1.1 2,243,309 + -1.1 2,243,330 - -0.8 2,243,431 + -0.5 2,243,487 - -0.2
Or see this region's nucleotide sequence