Strain Fitness in Shewanella oneidensis MR-1 around SO2070

Experiment: ShewMM_MES_buffer

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisF and hisA overlap by 19 nucleotideshisA and hisH are separated by 104 nucleotideshisH and hisB are separated by 144 nucleotides SO2068: hisF - hisF protein (cyclase) (NCBI ptt file), at 2,164,813 to 2,165,586 hisF SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305 hisA SO2070: hisH - amidotransferase HisH (NCBI ptt file), at 2,166,410 to 2,167,075 hisH SO2071: hisB - imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase (NCBI ptt file), at 2,167,220 to 2,168,311 hisB Position (kb) 2166 2167 2168Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 2165.421 kb on - strand, within hisFat 2165.528 kb on + strandat 2165.529 kb on - strandat 2165.561 kb on - strandat 2165.583 kb on + strandat 2165.602 kb on + strandat 2165.660 kb on + strand, within hisAat 2165.695 kb on - strand, within hisAat 2165.746 kb on - strand, within hisAat 2165.799 kb on - strand, within hisAat 2165.924 kb on + strand, within hisAat 2166.092 kb on + strand, within hisAat 2166.092 kb on + strand, within hisAat 2166.100 kb on - strand, within hisAat 2166.100 kb on - strand, within hisAat 2166.216 kb on + strand, within hisAat 2166.241 kb on + strandat 2166.311 kb on - strandat 2166.483 kb on + strand, within hisHat 2166.483 kb on + strand, within hisHat 2166.510 kb on + strand, within hisHat 2166.552 kb on - strand, within hisHat 2166.575 kb on - strand, within hisHat 2166.656 kb on + strand, within hisHat 2166.792 kb on + strand, within hisHat 2166.942 kb on + strand, within hisHat 2166.943 kb on + strand, within hisHat 2167.012 kb on + strandat 2167.130 kb on - strandat 2167.185 kb on + strandat 2167.193 kb on - strandat 2167.193 kb on - strandat 2167.225 kb on - strandat 2167.294 kb on - strandat 2167.305 kb on + strandat 2167.358 kb on + strand, within hisBat 2167.488 kb on + strand, within hisBat 2167.596 kb on - strand, within hisBat 2167.637 kb on - strand, within hisBat 2167.877 kb on - strand, within hisBat 2167.918 kb on + strand, within hisB

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Per-strain Table

Position Strand Gene LocusTag Fraction ShewMM_MES_buffer
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2,165,421 - hisF SO2068 0.79 -1.6
2,165,528 + -6.1
2,165,529 - -3.9
2,165,561 - -2.4
2,165,583 + -2.6
2,165,602 + -2.7
2,165,660 + hisA SO2069 0.12 -2.5
2,165,695 - hisA SO2069 0.17 -1.6
2,165,746 - hisA SO2069 0.24 -2.9
2,165,799 - hisA SO2069 0.31 -2.5
2,165,924 + hisA SO2069 0.48 -3.7
2,166,092 + hisA SO2069 0.71 -4.7
2,166,092 + hisA SO2069 0.71 -2.7
2,166,100 - hisA SO2069 0.72 -4.8
2,166,100 - hisA SO2069 0.72 -4.0
2,166,216 + hisA SO2069 0.88 -2.2
2,166,241 + -1.9
2,166,311 - -3.0
2,166,483 + hisH SO2070 0.11 -2.9
2,166,483 + hisH SO2070 0.11 -3.9
2,166,510 + hisH SO2070 0.15 -4.6
2,166,552 - hisH SO2070 0.21 -4.2
2,166,575 - hisH SO2070 0.25 -3.8
2,166,656 + hisH SO2070 0.37 -5.9
2,166,792 + hisH SO2070 0.57 -3.9
2,166,942 + hisH SO2070 0.80 -4.4
2,166,943 + hisH SO2070 0.80 -5.2
2,167,012 + -6.8
2,167,130 - -3.2
2,167,185 + -5.0
2,167,193 - +0.2
2,167,193 - -0.0
2,167,225 - +0.5
2,167,294 - -1.9
2,167,305 + -4.3
2,167,358 + hisB SO2071 0.13 -4.6
2,167,488 + hisB SO2071 0.25 -4.0
2,167,596 - hisB SO2071 0.34 -3.6
2,167,637 - hisB SO2071 0.38 -5.3
2,167,877 - hisB SO2071 0.60 -3.0
2,167,918 + hisB SO2071 0.64 -3.0

Or see this region's nucleotide sequence