Experiment: M5 outer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliK and fliJ are separated by 143 nucleotides fliJ and fliI are separated by 11 nucleotides fliI and fliH overlap by 32 nucleotides fliH and fliG overlap by 14 nucleotides
SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689
fliK
SO3224: fliJ - flagellar protein FliJ (NCBI ptt file), at 3,362,833 to 3,363,276
fliJ
SO3225: fliI - flagellum-specific ATP synthase FliI (NCBI ptt file), at 3,363,288 to 3,364,625
fliI
SO3226: fliH - flagellar assembly protein FliH (NCBI ptt file), at 3,364,594 to 3,365,544
fliH
SO3227: fliG - flagellar motor switch protein FliG (NCBI ptt file), at 3,365,531 to 3,366,577
fliG
Position (kb)
3363
3364
3365 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3362.496 kb on - strand, within fliK at 3362.695 kb on + strand at 3362.980 kb on - strand, within fliJ at 3363.084 kb on + strand, within fliJ at 3363.092 kb on + strand, within fliJ at 3363.100 kb on - strand, within fliJ at 3363.100 kb on - strand, within fliJ at 3363.338 kb on + strand at 3363.502 kb on + strand, within fliI at 3363.502 kb on + strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.510 kb on - strand, within fliI at 3363.608 kb on + strand, within fliI at 3363.613 kb on - strand, within fliI at 3363.690 kb on - strand, within fliI at 3363.707 kb on + strand, within fliI at 3363.707 kb on + strand, within fliI at 3363.935 kb on - strand, within fliI at 3363.935 kb on - strand, within fliI at 3364.071 kb on - strand, within fliI at 3364.096 kb on + strand, within fliI at 3364.235 kb on + strand, within fliI at 3364.314 kb on - strand, within fliI at 3364.553 kb on - strand at 3364.553 kb on - strand at 3365.035 kb on - strand, within fliH at 3365.071 kb on - strand, within fliH at 3365.076 kb on + strand, within fliH at 3365.084 kb on - strand, within fliH at 3365.084 kb on - strand, within fliH at 3365.113 kb on - strand, within fliH at 3365.204 kb on - strand, within fliH at 3365.239 kb on + strand, within fliH at 3365.246 kb on + strand, within fliH at 3365.249 kb on - strand, within fliH at 3365.264 kb on - strand, within fliH at 3365.292 kb on + strand, within fliH at 3365.299 kb on - strand, within fliH at 3365.300 kb on - strand, within fliH at 3365.417 kb on - strand, within fliH at 3365.417 kb on - strand, within fliH at 3365.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M5 outer remove 3,362,496 - fliK SO3223 0.88 -2.9 3,362,695 + -1.2 3,362,980 - fliJ SO3224 0.33 -1.3 3,363,084 + fliJ SO3224 0.57 -2.5 3,363,092 + fliJ SO3224 0.58 -3.5 3,363,100 - fliJ SO3224 0.60 -2.4 3,363,100 - fliJ SO3224 0.60 -1.2 3,363,338 + -2.9 3,363,502 + fliI SO3225 0.16 -3.1 3,363,502 + fliI SO3225 0.16 -1.5 3,363,510 - fliI SO3225 0.17 -1.3 3,363,510 - fliI SO3225 0.17 -3.3 3,363,510 - fliI SO3225 0.17 -2.3 3,363,510 - fliI SO3225 0.17 -0.9 3,363,608 + fliI SO3225 0.24 -2.2 3,363,613 - fliI SO3225 0.24 -1.4 3,363,690 - fliI SO3225 0.30 -3.1 3,363,707 + fliI SO3225 0.31 -0.2 3,363,707 + fliI SO3225 0.31 -2.7 3,363,935 - fliI SO3225 0.48 -0.6 3,363,935 - fliI SO3225 0.48 -1.3 3,364,071 - fliI SO3225 0.59 -0.6 3,364,096 + fliI SO3225 0.60 -1.7 3,364,235 + fliI SO3225 0.71 -1.8 3,364,314 - fliI SO3225 0.77 -1.3 3,364,553 - -0.3 3,364,553 - -2.6 3,365,035 - fliH SO3226 0.46 -1.3 3,365,071 - fliH SO3226 0.50 +0.5 3,365,076 + fliH SO3226 0.51 -2.1 3,365,084 - fliH SO3226 0.52 -2.5 3,365,084 - fliH SO3226 0.52 -1.5 3,365,113 - fliH SO3226 0.55 -2.2 3,365,204 - fliH SO3226 0.64 -2.8 3,365,239 + fliH SO3226 0.68 -0.8 3,365,246 + fliH SO3226 0.69 -4.1 3,365,249 - fliH SO3226 0.69 -1.9 3,365,264 - fliH SO3226 0.70 -1.9 3,365,292 + fliH SO3226 0.73 -2.3 3,365,299 - fliH SO3226 0.74 +0.0 3,365,300 - fliH SO3226 0.74 -2.7 3,365,417 - fliH SO3226 0.87 +0.0 3,365,417 - fliH SO3226 0.87 +1.0 3,365,583 + -1.3
Or see this region's nucleotide sequence