Experiment: M5 outer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1255 and SO1256 overlap by 12 nucleotides SO1256 and SO1258 are separated by 175 nucleotides SO1258 and SO1259 are separated by 135 nucleotides
SO1255: SO1255 - cyclic nucleotide phosphodiesterase, putative (NCBI ptt file), at 1,304,327 to 1,305,856
SO1255
SO1256: SO1256 - hypothetical 4-hydroxybenzoyl-CoA thioesterase (NCBI ptt file), at 1,305,845 to 1,306,258
SO1256
SO1258: SO1258 - adenylosuccinate synthetase, putative (NCBI ptt file), at 1,306,434 to 1,307,690
SO1258
SO1259: SO1259 - transcriptional regulator, LysR family (NCBI ptt file), at 1,307,826 to 1,308,731
SO1259
Position (kb)
1306
1307
1308 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1305.436 kb on + strand, within SO1255 at 1305.474 kb on + strand, within SO1255 at 1305.482 kb on - strand, within SO1255 at 1305.611 kb on - strand, within SO1255 at 1305.612 kb on - strand, within SO1255 at 1305.635 kb on + strand, within SO1255 at 1305.635 kb on + strand, within SO1255 at 1305.635 kb on + strand, within SO1255 at 1305.691 kb on - strand, within SO1255 at 1305.699 kb on + strand, within SO1255 at 1305.699 kb on - strand, within SO1255 at 1305.727 kb on + strand at 1305.749 kb on + strand at 1305.754 kb on + strand at 1305.757 kb on - strand at 1305.757 kb on - strand at 1305.798 kb on + strand at 1305.806 kb on - strand at 1305.813 kb on - strand at 1305.875 kb on - strand at 1305.915 kb on - strand, within SO1256 at 1305.922 kb on + strand, within SO1256 at 1306.056 kb on + strand, within SO1256 at 1306.071 kb on - strand, within SO1256 at 1306.294 kb on + strand at 1306.294 kb on + strand at 1306.321 kb on + strand at 1306.492 kb on - strand at 1306.524 kb on - strand at 1306.534 kb on - strand at 1306.569 kb on - strand, within SO1258 at 1306.715 kb on - strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.938 kb on + strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1306.946 kb on - strand, within SO1258 at 1307.070 kb on - strand, within SO1258 at 1307.150 kb on + strand, within SO1258 at 1307.167 kb on + strand, within SO1258 at 1307.284 kb on + strand, within SO1258 at 1307.301 kb on + strand, within SO1258 at 1307.364 kb on + strand, within SO1258 at 1307.374 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.379 kb on + strand, within SO1258 at 1307.382 kb on - strand, within SO1258 at 1307.384 kb on + strand, within SO1258 at 1307.384 kb on + strand, within SO1258 at 1307.537 kb on + strand, within SO1258 at 1307.687 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.903 kb on + strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.911 kb on - strand at 1307.913 kb on - strand at 1307.965 kb on + strand, within SO1259 at 1307.989 kb on + strand, within SO1259 at 1307.997 kb on - strand, within SO1259 at 1308.053 kb on - strand, within SO1259 at 1308.097 kb on + strand, within SO1259 at 1308.111 kb on - strand, within SO1259 at 1308.111 kb on - strand, within SO1259 at 1308.129 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.217 kb on + strand, within SO1259 at 1308.225 kb on - strand, within SO1259 at 1308.225 kb on - strand, within SO1259 at 1308.317 kb on - strand, within SO1259 at 1308.444 kb on + strand, within SO1259 at 1308.444 kb on + strand, within SO1259 at 1308.452 kb on - strand, within SO1259 at 1308.452 kb on - strand, within SO1259 at 1308.493 kb on + strand, within SO1259
Per-strain Table
Position Strand Gene LocusTag Fraction M5 outer remove 1,305,436 + SO1255 0.72 +0.0 1,305,474 + SO1255 0.75 +0.6 1,305,482 - SO1255 0.75 -2.3 1,305,611 - SO1255 0.84 +1.0 1,305,612 - SO1255 0.84 -0.9 1,305,635 + SO1255 0.85 -0.1 1,305,635 + SO1255 0.85 -2.6 1,305,635 + SO1255 0.85 +0.4 1,305,691 - SO1255 0.89 +0.1 1,305,699 + SO1255 0.90 -2.1 1,305,699 - SO1255 0.90 +1.1 1,305,727 + +0.2 1,305,749 + -0.6 1,305,754 + -0.7 1,305,757 - +1.7 1,305,757 - -1.6 1,305,798 + +0.2 1,305,806 - +0.8 1,305,813 - +0.0 1,305,875 - +0.1 1,305,915 - SO1256 0.17 -1.8 1,305,922 + SO1256 0.19 -1.3 1,306,056 + SO1256 0.51 +2.1 1,306,071 - SO1256 0.55 +3.3 1,306,294 + -0.5 1,306,294 + +0.8 1,306,321 + +0.7 1,306,492 - +1.1 1,306,524 - +0.9 1,306,534 - +1.3 1,306,569 - SO1258 0.11 +0.8 1,306,715 - SO1258 0.22 +0.3 1,306,938 + SO1258 0.40 +1.2 1,306,938 + SO1258 0.40 +0.6 1,306,938 + SO1258 0.40 +1.0 1,306,938 + SO1258 0.40 -0.7 1,306,938 + SO1258 0.40 +0.8 1,306,946 - SO1258 0.41 -0.3 1,306,946 - SO1258 0.41 +0.6 1,306,946 - SO1258 0.41 +1.9 1,307,070 - SO1258 0.51 -1.5 1,307,150 + SO1258 0.57 -1.1 1,307,167 + SO1258 0.58 +2.1 1,307,284 + SO1258 0.68 +1.2 1,307,301 + SO1258 0.69 +0.7 1,307,364 + SO1258 0.74 -1.1 1,307,374 + SO1258 0.75 +1.6 1,307,379 + SO1258 0.75 -0.3 1,307,379 + SO1258 0.75 -1.3 1,307,379 + SO1258 0.75 +0.9 1,307,379 + SO1258 0.75 -1.6 1,307,379 + SO1258 0.75 -1.0 1,307,379 + SO1258 0.75 +0.5 1,307,382 - SO1258 0.75 +1.5 1,307,384 + SO1258 0.76 +0.8 1,307,384 + SO1258 0.76 +0.1 1,307,537 + SO1258 0.88 -0.5 1,307,687 + -0.6 1,307,903 + -0.4 1,307,903 + +1.7 1,307,903 + +0.0 1,307,903 + +0.4 1,307,911 - -0.9 1,307,911 - +0.1 1,307,911 - +0.4 1,307,911 - +1.6 1,307,911 - +1.4 1,307,911 - -1.0 1,307,913 - +0.0 1,307,965 + SO1259 0.15 -1.5 1,307,989 + SO1259 0.18 -0.2 1,307,997 - SO1259 0.19 +0.5 1,308,053 - SO1259 0.25 -0.2 1,308,097 + SO1259 0.30 -0.8 1,308,111 - SO1259 0.31 -1.1 1,308,111 - SO1259 0.31 -0.5 1,308,129 + SO1259 0.33 -0.4 1,308,217 + SO1259 0.43 +0.1 1,308,217 + SO1259 0.43 -2.5 1,308,217 + SO1259 0.43 +0.6 1,308,217 + SO1259 0.43 -0.1 1,308,217 + SO1259 0.43 -2.8 1,308,225 - SO1259 0.44 -0.1 1,308,225 - SO1259 0.44 +0.2 1,308,317 - SO1259 0.54 -0.3 1,308,444 + SO1259 0.68 -1.3 1,308,444 + SO1259 0.68 +0.7 1,308,452 - SO1259 0.69 -1.5 1,308,452 - SO1259 0.69 -0.5 1,308,493 + SO1259 0.74 +0.9
Or see this region's nucleotide sequence