Experiment: M5 outer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0754 and SO0755 are separated by 526 nucleotides SO0755 and aroG are separated by 49 nucleotides aroG and SO0757 are separated by 236 nucleotides
SO0754: SO0754 - ABC transporter, ATP-binding protein (NCBI ptt file), at 769,396 to 771,186
SO0754
SO0755: SO0755 - hypothetical protein (NCBI ptt file), at 771,713 to 771,889
SO0755
SO0756: aroG - phospho-2-dehydro-3-deoxyheptonate aldolase, phe-sensitive (NCBI ptt file), at 771,939 to 772,997
aroG
SO0757: SO0757 - TPR domain protein (NCBI ptt file), at 773,234 to 774,421
SO0757
Position (kb)
771
772
773 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 770.963 kb on - strand, within SO0754 at 770.981 kb on - strand, within SO0754 at 770.990 kb on + strand, within SO0754 at 771.069 kb on + strand at 771.140 kb on + strand at 771.140 kb on + strand at 771.174 kb on + strand at 771.174 kb on + strand at 771.189 kb on + strand at 771.189 kb on + strand at 771.197 kb on - strand at 771.233 kb on + strand at 771.241 kb on - strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.299 kb on + strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.307 kb on - strand at 771.336 kb on - strand at 771.343 kb on + strand at 771.343 kb on + strand at 771.401 kb on - strand at 771.402 kb on - strand at 771.403 kb on + strand at 771.403 kb on + strand at 771.411 kb on - strand at 771.411 kb on - strand at 771.411 kb on - strand at 771.571 kb on - strand at 771.625 kb on - strand at 771.629 kb on + strand at 771.713 kb on + strand at 771.914 kb on - strand at 771.927 kb on + strand at 771.937 kb on + strand at 772.032 kb on - strand at 772.041 kb on + strand at 772.041 kb on + strand at 772.067 kb on + strand, within aroG at 772.074 kb on + strand, within aroG at 772.196 kb on + strand, within aroG at 772.196 kb on + strand, within aroG at 772.283 kb on - strand, within aroG at 772.466 kb on - strand, within aroG at 772.466 kb on - strand, within aroG at 772.525 kb on - strand, within aroG at 772.527 kb on - strand, within aroG at 772.550 kb on + strand, within aroG at 772.574 kb on - strand, within aroG at 772.587 kb on + strand, within aroG at 772.587 kb on + strand, within aroG at 772.611 kb on + strand, within aroG at 772.613 kb on - strand, within aroG at 772.641 kb on - strand, within aroG at 772.697 kb on - strand, within aroG at 772.963 kb on - strand at 773.037 kb on - strand at 773.050 kb on + strand at 773.050 kb on + strand at 773.231 kb on + strand at 773.232 kb on - strand at 773.239 kb on - strand at 773.258 kb on - strand at 773.363 kb on + strand, within SO0757 at 773.397 kb on + strand, within SO0757 at 773.446 kb on - strand, within SO0757 at 773.451 kb on + strand, within SO0757 at 773.459 kb on - strand, within SO0757 at 773.554 kb on - strand, within SO0757 at 773.581 kb on + strand, within SO0757 at 773.715 kb on + strand, within SO0757 at 773.723 kb on - strand, within SO0757 at 773.726 kb on + strand, within SO0757 at 773.727 kb on + strand, within SO0757 at 773.727 kb on + strand, within SO0757 at 773.836 kb on + strand, within SO0757 at 773.844 kb on - strand, within SO0757 at 773.961 kb on + strand, within SO0757 at 773.978 kb on - strand, within SO0757
Per-strain Table
Position Strand Gene LocusTag Fraction M5 outer remove 770,963 - SO0754 0.87 -0.7 770,981 - SO0754 0.88 -1.6 770,990 + SO0754 0.89 +1.7 771,069 + -1.0 771,140 + +0.4 771,140 + +2.1 771,174 + +1.2 771,174 + +0.4 771,189 + -1.2 771,189 + -3.7 771,197 - -0.4 771,233 + +0.1 771,241 - +0.9 771,299 + +2.3 771,299 + +1.8 771,299 + -0.4 771,299 + -1.8 771,299 + +0.4 771,299 + +0.7 771,299 + +1.4 771,307 - +1.3 771,307 - -2.1 771,307 - -1.2 771,307 - -1.5 771,307 - +1.7 771,307 - -2.7 771,307 - -2.1 771,307 - -0.4 771,307 - -1.3 771,336 - -1.3 771,343 + +1.7 771,343 + -1.0 771,401 - -0.4 771,402 - +1.3 771,403 + -1.4 771,403 + -1.5 771,411 - -3.1 771,411 - -1.0 771,411 - -1.7 771,571 - -0.3 771,625 - -0.8 771,629 + -0.3 771,713 + +1.7 771,914 - +1.6 771,927 + -1.1 771,937 + -1.6 772,032 - -0.1 772,041 + -0.9 772,041 + +3.1 772,067 + aroG SO0756 0.12 +0.2 772,074 + aroG SO0756 0.13 +0.3 772,196 + aroG SO0756 0.24 +1.0 772,196 + aroG SO0756 0.24 +0.3 772,283 - aroG SO0756 0.32 -3.7 772,466 - aroG SO0756 0.50 +2.4 772,466 - aroG SO0756 0.50 +0.8 772,525 - aroG SO0756 0.55 +0.7 772,527 - aroG SO0756 0.56 -0.7 772,550 + aroG SO0756 0.58 +1.5 772,574 - aroG SO0756 0.60 +0.6 772,587 + aroG SO0756 0.61 -2.3 772,587 + aroG SO0756 0.61 +0.2 772,611 + aroG SO0756 0.63 +0.1 772,613 - aroG SO0756 0.64 +1.6 772,641 - aroG SO0756 0.66 +0.4 772,697 - aroG SO0756 0.72 -0.5 772,963 - +0.1 773,037 - -0.7 773,050 + +1.1 773,050 + -1.3 773,231 + -0.3 773,232 - +0.5 773,239 - -1.0 773,258 - +1.5 773,363 + SO0757 0.11 +1.4 773,397 + SO0757 0.14 +0.1 773,446 - SO0757 0.18 -1.0 773,451 + SO0757 0.18 -2.8 773,459 - SO0757 0.19 -0.4 773,554 - SO0757 0.27 +1.5 773,581 + SO0757 0.29 -0.6 773,715 + SO0757 0.40 +0.2 773,723 - SO0757 0.41 +0.6 773,726 + SO0757 0.41 +1.8 773,727 + SO0757 0.41 +1.1 773,727 + SO0757 0.41 -0.2 773,836 + SO0757 0.51 -1.4 773,844 - SO0757 0.51 -1.0 773,961 + SO0757 0.61 +0.1 773,978 - SO0757 0.63 +0.8
Or see this region's nucleotide sequence