Experiment: M2 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliL are separated by 39 nucleotides fliL and fliK are separated by 82 nucleotides
SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459
fliM
SO3222: fliL - flagellar protein FliL (NCBI ptt file), at 3,360,499 to 3,361,023
fliL
SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689
fliK
Position (kb)
3360
3361
3362 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 3359.518 kb on + strand at 3359.684 kb on - strand, within fliM at 3359.732 kb on - strand, within fliM at 3359.742 kb on - strand, within fliM at 3359.829 kb on - strand, within fliM at 3359.894 kb on + strand, within fliM at 3359.909 kb on + strand, within fliM at 3359.947 kb on + strand, within fliM at 3359.955 kb on - strand, within fliM at 3359.966 kb on + strand, within fliM at 3359.974 kb on - strand, within fliM at 3360.006 kb on - strand, within fliM at 3360.011 kb on - strand, within fliM at 3360.157 kb on + strand, within fliM at 3360.237 kb on - strand, within fliM at 3360.327 kb on + strand, within fliM at 3360.415 kb on + strand at 3360.426 kb on + strand at 3360.653 kb on - strand, within fliL at 3360.653 kb on - strand, within fliL at 3360.772 kb on - strand, within fliL at 3360.803 kb on - strand, within fliL at 3360.858 kb on - strand, within fliL at 3360.947 kb on - strand, within fliL at 3361.019 kb on + strand at 3361.024 kb on + strand at 3361.027 kb on - strand at 3361.058 kb on + strand at 3361.235 kb on + strand at 3361.251 kb on + strand at 3361.259 kb on - strand at 3361.259 kb on - strand at 3361.346 kb on + strand, within fliK at 3361.623 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.638 kb on + strand, within fliK at 3361.640 kb on + strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.646 kb on - strand, within fliK at 3361.804 kb on - strand, within fliK at 3361.806 kb on + strand, within fliK at 3361.918 kb on + strand, within fliK
Per-strain Table
Position Strand Gene LocusTag Fraction M2 center remove 3,359,518 + +1.4 3,359,684 - fliM SO3221 0.25 -2.4 3,359,732 - fliM SO3221 0.29 -1.5 3,359,742 - fliM SO3221 0.30 -0.6 3,359,829 - fliM SO3221 0.39 -1.9 3,359,894 + fliM SO3221 0.45 -0.5 3,359,909 + fliM SO3221 0.47 +3.5 3,359,947 + fliM SO3221 0.50 -1.6 3,359,955 - fliM SO3221 0.51 -2.5 3,359,966 + fliM SO3221 0.52 +1.2 3,359,974 - fliM SO3221 0.53 -3.5 3,360,006 - fliM SO3221 0.56 +2.1 3,360,011 - fliM SO3221 0.56 -1.1 3,360,157 + fliM SO3221 0.71 -2.3 3,360,237 - fliM SO3221 0.78 +1.0 3,360,327 + fliM SO3221 0.87 -2.3 3,360,415 + +2.0 3,360,426 + +1.5 3,360,653 - fliL SO3222 0.29 -3.7 3,360,653 - fliL SO3222 0.29 -3.6 3,360,772 - fliL SO3222 0.52 -3.5 3,360,803 - fliL SO3222 0.58 -3.5 3,360,858 - fliL SO3222 0.68 -0.9 3,360,947 - fliL SO3222 0.85 -0.5 3,361,019 + +2.2 3,361,024 + +1.8 3,361,027 - -3.0 3,361,058 + +4.1 3,361,235 + -2.5 3,361,251 + -1.8 3,361,259 - -1.1 3,361,259 - -2.1 3,361,346 + fliK SO3223 0.15 -2.5 3,361,623 + fliK SO3223 0.33 -2.1 3,361,638 + fliK SO3223 0.34 -4.1 3,361,638 + fliK SO3223 0.34 -1.7 3,361,638 + fliK SO3223 0.34 -1.5 3,361,640 + fliK SO3223 0.34 -2.4 3,361,646 - fliK SO3223 0.34 -3.7 3,361,646 - fliK SO3223 0.34 -2.8 3,361,646 - fliK SO3223 0.34 -3.9 3,361,646 - fliK SO3223 0.34 -5.1 3,361,646 - fliK SO3223 0.34 -2.7 3,361,804 - fliK SO3223 0.44 -1.6 3,361,806 + fliK SO3223 0.44 -4.0 3,361,918 + fliK SO3223 0.51 -2.0
Or see this region's nucleotide sequence