Experiment: M2 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2872 and SO2874 are separated by 303 nucleotides SO2874 and SO2875 overlap by 11 nucleotides SO2875 and SO2876 are separated by 62 nucleotides
SO2872: SO2872 - conserved hypothetical protein (NCBI ptt file), at 2,993,623 to 2,993,997
SO2872
SO2874: SO2874 - ISSod10, transposase OrfA (NCBI ptt file), at 2,994,301 to 2,994,780
SO2874
SO2875: SO2875 - ISSod10, transposase OrfB (NCBI ptt file), at 2,994,770 to 2,995,327
SO2875
SO2876: SO2876 - hypothetical protein (NCBI ptt file), at 2,995,390 to 2,997,342
SO2876
Position (kb)
2994
2995
2996 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2993.794 kb on + strand, within SO2872 at 2993.910 kb on - strand, within SO2872 at 2994.217 kb on + strand at 2994.225 kb on - strand at 2995.383 kb on + strand at 2995.447 kb on - strand at 2995.595 kb on + strand, within SO2876 at 2995.603 kb on - strand, within SO2876 at 2995.714 kb on + strand, within SO2876 at 2995.714 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.065 kb on + strand, within SO2876 at 2996.073 kb on - strand, within SO2876 at 2996.127 kb on - strand, within SO2876 at 2996.161 kb on + strand, within SO2876 at 2996.202 kb on + strand, within SO2876
Per-strain Table
Position Strand Gene LocusTag Fraction M2 center remove 2,993,794 + SO2872 0.46 +0.2 2,993,910 - SO2872 0.77 +2.5 2,994,217 + -1.8 2,994,225 - -0.9 2,995,383 + +0.3 2,995,447 - -0.3 2,995,595 + SO2876 0.10 -0.8 2,995,603 - SO2876 0.11 +0.6 2,995,714 + SO2876 0.17 -1.2 2,995,714 + SO2876 0.17 +0.0 2,996,065 + SO2876 0.35 -0.4 2,996,065 + SO2876 0.35 +0.1 2,996,065 + SO2876 0.35 -2.6 2,996,073 - SO2876 0.35 +0.3 2,996,127 - SO2876 0.38 +1.2 2,996,161 + SO2876 0.39 +2.1 2,996,202 + SO2876 0.42 +0.7
Or see this region's nucleotide sequence