Experiment: M2 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2306 and lolA are separated by 69 nucleotides lolA and SO2308 are separated by 2 nucleotides SO2308 and crcB overlap by 8 nucleotides crcB and serS are separated by 18 nucleotides
SO2306: SO2306 - cell division protein FtsK, putative (NCBI ptt file), at 2,417,093 to 2,419,828
SO2306
SO2307: lolA - outer membrane lipoprotein carrier protein LolA (NCBI ptt file), at 2,419,898 to 2,420,518
lolA
SO2308: SO2308 - ATPase, AAA family (NCBI ptt file), at 2,420,521 to 2,421,858
SO2308
SO2309: crcB - crcB protein (NCBI ptt file), at 2,421,851 to 2,422,225
crcB
SO2310: serS - seryl-tRNA synthetase (NCBI ptt file), at 2,422,244 to 2,423,530
serS
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-2
-1
0
1 at 2419.526 kb on + strand, within SO2306 at 2420.649 kb on - strand at 2421.046 kb on - strand, within SO2308 at 2421.190 kb on - strand, within SO2308 at 2421.549 kb on + strand, within SO2308 at 2421.612 kb on - strand, within SO2308 at 2421.689 kb on + strand, within SO2308 at 2421.699 kb on + strand, within SO2308 at 2421.826 kb on + strand at 2421.867 kb on - strand at 2421.874 kb on - strand at 2421.877 kb on + strand at 2421.995 kb on + strand, within crcB
Per-strain Table
Position Strand Gene LocusTag Fraction M2 center remove 2,419,526 + SO2306 0.89 -2.2 2,420,649 - -0.5 2,421,046 - SO2308 0.39 +0.0 2,421,190 - SO2308 0.50 -0.9 2,421,549 + SO2308 0.77 -1.4 2,421,612 - SO2308 0.82 +0.1 2,421,689 + SO2308 0.87 +1.1 2,421,699 + SO2308 0.88 +0.6 2,421,826 + -0.7 2,421,867 - -0.9 2,421,874 - +1.6 2,421,877 + -0.6 2,421,995 + crcB SO2309 0.38 +0.6
Or see this region's nucleotide sequence