Experiment: M2 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lepB and rnc are separated by 1 nucleotides rnc and era overlap by 4 nucleotides era and recO are separated by 54 nucleotides recO and pdxJ are separated by 84 nucleotides
SO1347: lepB - signal peptidase I (NCBI ptt file), at 1,404,007 to 1,404,924
lepB
SO1348: rnc - ribonuclease III (NCBI ptt file), at 1,404,926 to 1,405,606
rnc
SO1349: era - GTP-binding protein Era (NCBI ptt file), at 1,405,603 to 1,406,622
era
SO1350: recO - DNA repair protein RecO (NCBI ptt file), at 1,406,677 to 1,407,378
recO
SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200
pdxJ
Position (kb)
1405
1406
1407 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1404.608 kb on + strand, within lepB at 1404.764 kb on + strand, within lepB at 1404.875 kb on + strand at 1406.608 kb on - strand at 1406.637 kb on - strand at 1406.643 kb on + strand at 1406.651 kb on - strand at 1406.685 kb on + strand at 1406.692 kb on + strand at 1406.981 kb on - strand, within recO at 1407.049 kb on - strand, within recO at 1407.124 kb on + strand, within recO at 1407.126 kb on + strand, within recO at 1407.131 kb on + strand, within recO at 1407.314 kb on + strand at 1407.360 kb on - strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M2 center remove 1,404,608 + lepB SO1347 0.65 -2.0 1,404,764 + lepB SO1347 0.82 -3.4 1,404,875 + +1.1 1,406,608 - -2.5 1,406,637 - -2.8 1,406,643 + -2.5 1,406,651 - +0.7 1,406,685 + +0.5 1,406,692 + -0.7 1,406,981 - recO SO1350 0.43 -1.6 1,407,049 - recO SO1350 0.53 -2.5 1,407,124 + recO SO1350 0.64 -0.5 1,407,126 + recO SO1350 0.64 -1.0 1,407,131 + recO SO1350 0.65 -0.7 1,407,314 + +0.3 1,407,360 - -0.7 1,407,410 + +0.0 1,407,410 + +0.3 1,407,410 + -1.2 1,407,410 + +0.0 1,407,418 - -0.5
Or see this region's nucleotide sequence