Strain Fitness in Shewanella oneidensis MR-1 around SO1349

Experiment: M2 center

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlepB and rnc are separated by 1 nucleotidesrnc and era overlap by 4 nucleotidesera and recO are separated by 54 nucleotidesrecO and pdxJ are separated by 84 nucleotides SO1347: lepB - signal peptidase I (NCBI ptt file), at 1,404,007 to 1,404,924 lepB SO1348: rnc - ribonuclease III (NCBI ptt file), at 1,404,926 to 1,405,606 rnc SO1349: era - GTP-binding protein Era (NCBI ptt file), at 1,405,603 to 1,406,622 era SO1350: recO - DNA repair protein RecO (NCBI ptt file), at 1,406,677 to 1,407,378 recO SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200 pdxJ Position (kb) 1405 1406 1407Strain fitness (log2 ratio) -3 -2 -1 0 1at 1404.608 kb on + strand, within lepBat 1404.764 kb on + strand, within lepBat 1404.875 kb on + strandat 1406.608 kb on - strandat 1406.637 kb on - strandat 1406.643 kb on + strandat 1406.651 kb on - strandat 1406.685 kb on + strandat 1406.692 kb on + strandat 1406.981 kb on - strand, within recOat 1407.049 kb on - strand, within recOat 1407.124 kb on + strand, within recOat 1407.126 kb on + strand, within recOat 1407.131 kb on + strand, within recOat 1407.314 kb on + strandat 1407.360 kb on - strandat 1407.410 kb on + strandat 1407.410 kb on + strandat 1407.410 kb on + strandat 1407.410 kb on + strandat 1407.418 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M2 center
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1,404,608 + lepB SO1347 0.65 -2.0
1,404,764 + lepB SO1347 0.82 -3.4
1,404,875 + +1.1
1,406,608 - -2.5
1,406,637 - -2.8
1,406,643 + -2.5
1,406,651 - +0.7
1,406,685 + +0.5
1,406,692 + -0.7
1,406,981 - recO SO1350 0.43 -1.6
1,407,049 - recO SO1350 0.53 -2.5
1,407,124 + recO SO1350 0.64 -0.5
1,407,126 + recO SO1350 0.64 -1.0
1,407,131 + recO SO1350 0.65 -0.7
1,407,314 + +0.3
1,407,360 - -0.7
1,407,410 + +0.0
1,407,410 + +0.3
1,407,410 + -1.2
1,407,410 + +0.0
1,407,418 - -0.5

Or see this region's nucleotide sequence