Experiment: M1 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dut and SO4251 are separated by 31 nucleotides SO4251 and SO4252 are separated by 134 nucleotides SO4252 and folE are separated by 267 nucleotides
SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761
dut
SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386
SO4251
SO4252: SO4252 - prolyl oligopeptidase family protein (NCBI ptt file), at 4,428,521 to 4,430,509
SO4252
SO4254: folE - GTP cyclohydrolase I (NCBI ptt file), at 4,430,777 to 4,431,427
folE
Position (kb)
4428
4429
4430
4431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.546 kb on - strand, within dut at 4427.622 kb on + strand, within dut at 4427.665 kb on - strand, within dut at 4427.791 kb on - strand at 4427.795 kb on + strand at 4427.840 kb on - strand at 4427.840 kb on - strand at 4427.863 kb on + strand, within SO4251 at 4427.908 kb on + strand, within SO4251 at 4427.993 kb on + strand, within SO4251 at 4428.036 kb on + strand, within SO4251 at 4428.057 kb on + strand, within SO4251 at 4428.232 kb on - strand, within SO4251 at 4428.252 kb on + strand, within SO4251 at 4428.338 kb on - strand at 4428.655 kb on - strand at 4428.777 kb on + strand, within SO4252 at 4428.795 kb on + strand, within SO4252 at 4428.803 kb on - strand, within SO4252 at 4428.805 kb on + strand, within SO4252 at 4428.837 kb on - strand, within SO4252 at 4428.871 kb on - strand, within SO4252 at 4428.985 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.039 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.104 kb on - strand, within SO4252 at 4429.151 kb on + strand, within SO4252 at 4429.189 kb on - strand, within SO4252 at 4429.200 kb on - strand, within SO4252 at 4429.229 kb on + strand, within SO4252 at 4429.255 kb on + strand, within SO4252 at 4429.313 kb on - strand, within SO4252 at 4429.342 kb on - strand, within SO4252 at 4429.502 kb on - strand, within SO4252 at 4429.527 kb on - strand, within SO4252 at 4429.558 kb on + strand, within SO4252 at 4429.634 kb on - strand, within SO4252 at 4429.698 kb on - strand, within SO4252 at 4429.711 kb on + strand, within SO4252 at 4429.711 kb on + strand, within SO4252 at 4429.735 kb on + strand, within SO4252 at 4429.743 kb on - strand, within SO4252 at 4429.743 kb on - strand, within SO4252 at 4429.743 kb on - strand, within SO4252 at 4429.790 kb on + strand, within SO4252 at 4429.803 kb on - strand, within SO4252 at 4429.848 kb on + strand, within SO4252 at 4429.911 kb on + strand, within SO4252 at 4430.039 kb on + strand, within SO4252 at 4430.039 kb on + strand, within SO4252 at 4430.040 kb on + strand, within SO4252 at 4430.047 kb on - strand, within SO4252 at 4430.047 kb on - strand, within SO4252 at 4430.047 kb on - strand, within SO4252 at 4430.095 kb on + strand, within SO4252 at 4430.160 kb on - strand, within SO4252 at 4430.167 kb on - strand, within SO4252 at 4430.172 kb on - strand, within SO4252 at 4430.184 kb on - strand, within SO4252 at 4430.238 kb on + strand, within SO4252 at 4430.248 kb on + strand, within SO4252 at 4430.308 kb on + strand, within SO4252 at 4430.308 kb on + strand, within SO4252 at 4430.308 kb on + strand, within SO4252 at 4430.316 kb on - strand at 4430.424 kb on - strand at 4430.434 kb on - strand at 4430.468 kb on + strand at 4430.658 kb on + strand at 4430.658 kb on + strand at 4430.658 kb on + strand at 4430.658 kb on + strand at 4430.659 kb on + strand at 4430.659 kb on + strand at 4430.659 kb on + strand at 4430.659 kb on + strand at 4430.659 kb on + strand at 4430.729 kb on + strand at 4430.729 kb on + strand at 4430.729 kb on + strand at 4431.425 kb on - strand at 4431.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M1 center remove 4,427,522 - dut SO4250 0.48 -3.5 4,427,522 - dut SO4250 0.48 -0.3 4,427,522 - dut SO4250 0.48 -1.5 4,427,546 - dut SO4250 0.53 -2.4 4,427,622 + dut SO4250 0.69 -2.7 4,427,665 - dut SO4250 0.79 -2.9 4,427,791 - -2.0 4,427,795 + -1.0 4,427,840 - -3.2 4,427,840 - -0.8 4,427,863 + SO4251 0.12 -3.8 4,427,908 + SO4251 0.19 -3.0 4,427,993 + SO4251 0.34 -3.5 4,428,036 + SO4251 0.41 -1.5 4,428,057 + SO4251 0.44 -1.1 4,428,232 - SO4251 0.74 -0.4 4,428,252 + SO4251 0.77 -2.1 4,428,338 - -2.9 4,428,655 - -0.4 4,428,777 + SO4252 0.13 -2.0 4,428,795 + SO4252 0.14 +0.2 4,428,803 - SO4252 0.14 +0.3 4,428,805 + SO4252 0.14 -1.6 4,428,837 - SO4252 0.16 +2.2 4,428,871 - SO4252 0.18 -0.9 4,428,985 + SO4252 0.23 -1.0 4,429,036 + SO4252 0.26 +0.2 4,429,036 + SO4252 0.26 +0.8 4,429,039 - SO4252 0.26 -2.5 4,429,044 - SO4252 0.26 +1.3 4,429,044 - SO4252 0.26 +0.5 4,429,104 - SO4252 0.29 +1.3 4,429,151 + SO4252 0.32 -0.8 4,429,189 - SO4252 0.34 +1.8 4,429,200 - SO4252 0.34 +1.1 4,429,229 + SO4252 0.36 -1.0 4,429,255 + SO4252 0.37 +0.3 4,429,313 - SO4252 0.40 +1.6 4,429,342 - SO4252 0.41 +2.2 4,429,502 - SO4252 0.49 +2.7 4,429,527 - SO4252 0.51 +0.7 4,429,558 + SO4252 0.52 +1.1 4,429,634 - SO4252 0.56 +0.7 4,429,698 - SO4252 0.59 -1.2 4,429,711 + SO4252 0.60 -1.1 4,429,711 + SO4252 0.60 +1.4 4,429,735 + SO4252 0.61 -0.2 4,429,743 - SO4252 0.61 -0.4 4,429,743 - SO4252 0.61 -0.1 4,429,743 - SO4252 0.61 +1.0 4,429,790 + SO4252 0.64 +0.8 4,429,803 - SO4252 0.64 -0.5 4,429,848 + SO4252 0.67 +0.1 4,429,911 + SO4252 0.70 +1.4 4,430,039 + SO4252 0.76 +0.2 4,430,039 + SO4252 0.76 -0.6 4,430,040 + SO4252 0.76 +0.5 4,430,047 - SO4252 0.77 +1.4 4,430,047 - SO4252 0.77 -1.0 4,430,047 - SO4252 0.77 +1.2 4,430,095 + SO4252 0.79 +0.2 4,430,160 - SO4252 0.82 +0.7 4,430,167 - SO4252 0.83 -0.7 4,430,172 - SO4252 0.83 -0.1 4,430,184 - SO4252 0.84 -2.2 4,430,238 + SO4252 0.86 +0.9 4,430,248 + SO4252 0.87 -2.7 4,430,308 + SO4252 0.90 -0.2 4,430,308 + SO4252 0.90 +1.7 4,430,308 + SO4252 0.90 +1.3 4,430,316 - -0.3 4,430,424 - +0.2 4,430,434 - +0.9 4,430,468 + +1.7 4,430,658 + -1.4 4,430,658 + +1.3 4,430,658 + -1.1 4,430,658 + +0.6 4,430,659 + +0.2 4,430,659 + -0.1 4,430,659 + +1.4 4,430,659 + +1.7 4,430,659 + +0.5 4,430,729 + -0.1 4,430,729 + -0.3 4,430,729 + +0.7 4,431,425 - +0.6 4,431,430 - -0.2
Or see this region's nucleotide sequence