Experiment: M1 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3015 and SO3016 are separated by 189 nucleotides SO3016 and SO3017 overlap by 4 nucleotides SO3017 and trpE are separated by 371 nucleotides
SO3015: SO3015 - conserved hypothetical protein (NCBI ptt file), at 3,127,031 to 3,127,819
SO3015
SO3016: SO3016 - Sua5/YciO/YrdC/YwlC family protein (NCBI ptt file), at 3,128,009 to 3,128,629
SO3016
SO3017: SO3017 - TrpH family protein (NCBI ptt file), at 3,128,626 to 3,129,486
SO3017
SO3019: trpE - anthranilate synthase component I (NCBI ptt file), at 3,129,858 to 3,131,582
trpE
Position (kb)
3128
3129
3130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.845 kb on + strand at 3127.853 kb on - strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.978 kb on + strand at 3127.986 kb on - strand at 3127.998 kb on - strand at 3128.029 kb on + strand at 3128.168 kb on - strand, within SO3016 at 3128.234 kb on - strand, within SO3016 at 3128.244 kb on + strand, within SO3016 at 3128.344 kb on + strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.352 kb on - strand, within SO3016 at 3128.389 kb on + strand, within SO3016 at 3128.513 kb on + strand, within SO3016 at 3128.598 kb on - strand at 3128.869 kb on - strand, within SO3017 at 3128.891 kb on - strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.902 kb on + strand, within SO3017 at 3128.910 kb on - strand, within SO3017 at 3128.937 kb on + strand, within SO3017 at 3128.984 kb on + strand, within SO3017 at 3129.005 kb on + strand, within SO3017 at 3129.047 kb on - strand, within SO3017 at 3129.073 kb on + strand, within SO3017 at 3129.119 kb on + strand, within SO3017 at 3129.177 kb on + strand, within SO3017 at 3129.197 kb on - strand, within SO3017 at 3129.222 kb on + strand, within SO3017 at 3129.230 kb on - strand, within SO3017 at 3129.267 kb on - strand, within SO3017 at 3129.337 kb on + strand, within SO3017 at 3129.488 kb on + strand at 3129.686 kb on - strand at 3129.714 kb on + strand at 3129.740 kb on + strand at 3129.741 kb on - strand at 3129.748 kb on - strand at 3129.748 kb on - strand at 3129.913 kb on + strand at 3130.075 kb on - strand, within trpE at 3130.113 kb on + strand, within trpE at 3130.215 kb on + strand, within trpE at 3130.215 kb on + strand, within trpE at 3130.223 kb on - strand, within trpE at 3130.223 kb on - strand, within trpE at 3130.320 kb on + strand, within trpE at 3130.360 kb on - strand, within trpE
Per-strain Table
Position Strand Gene LocusTag Fraction M1 center remove 3,127,845 + +2.1 3,127,845 + +0.7 3,127,845 + -1.4 3,127,845 + +0.5 3,127,853 - +0.9 3,127,978 + -0.2 3,127,978 + +1.0 3,127,978 + -0.0 3,127,978 + +0.7 3,127,986 - +1.7 3,127,998 - +1.7 3,128,029 + -0.3 3,128,168 - SO3016 0.26 -1.4 3,128,234 - SO3016 0.36 -0.4 3,128,244 + SO3016 0.38 +0.9 3,128,344 + SO3016 0.54 +1.3 3,128,352 - SO3016 0.55 +0.2 3,128,352 - SO3016 0.55 -0.4 3,128,352 - SO3016 0.55 +0.1 3,128,389 + SO3016 0.61 -0.4 3,128,513 + SO3016 0.81 +0.3 3,128,598 - -0.7 3,128,869 - SO3017 0.28 -1.1 3,128,891 - SO3017 0.31 -1.7 3,128,902 + SO3017 0.32 -0.7 3,128,902 + SO3017 0.32 -1.1 3,128,902 + SO3017 0.32 -0.7 3,128,910 - SO3017 0.33 +0.6 3,128,937 + SO3017 0.36 -0.8 3,128,984 + SO3017 0.42 +0.4 3,129,005 + SO3017 0.44 -0.5 3,129,047 - SO3017 0.49 +0.1 3,129,073 + SO3017 0.52 -0.7 3,129,119 + SO3017 0.57 -1.2 3,129,177 + SO3017 0.64 +1.1 3,129,197 - SO3017 0.66 +1.2 3,129,222 + SO3017 0.69 -1.3 3,129,230 - SO3017 0.70 -0.7 3,129,267 - SO3017 0.74 +0.7 3,129,337 + SO3017 0.83 -0.5 3,129,488 + +0.6 3,129,686 - -3.5 3,129,714 + -0.0 3,129,740 + -2.7 3,129,741 - -1.7 3,129,748 - -0.1 3,129,748 - -1.6 3,129,913 + +1.0 3,130,075 - trpE SO3019 0.13 -0.2 3,130,113 + trpE SO3019 0.15 +0.1 3,130,215 + trpE SO3019 0.21 +1.5 3,130,215 + trpE SO3019 0.21 +1.6 3,130,223 - trpE SO3019 0.21 +1.2 3,130,223 - trpE SO3019 0.21 -1.3 3,130,320 + trpE SO3019 0.27 -0.1 3,130,360 - trpE SO3019 0.29 -0.3
Or see this region's nucleotide sequence