Experiment: M1 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0429 and SO0430 are separated by 317 nucleotides SO0430 and SO0431 are separated by 52 nucleotides SO0431 and acnB are separated by 421 nucleotides
SO0429: SO0429 - peptidase, M13 family (NCBI ptt file), at 451,966 to 454,008
SO0429
SO0430: SO0430 - conserved hypothetical protein (NCBI ptt file), at 454,326 to 454,652
SO0430
SO0431: SO0431 - HAD-superfamily hydrolase, subfamily IA, variant 3 protein family (NCBI ptt file), at 454,705 to 455,358
SO0431
SO0432: acnB - aconitate hydratase 2 (NCBI ptt file), at 455,780 to 458,377
acnB
Position (kb) 454
455
456 Strain fitness (log2 ratio) -1
0
1
2 at 453.727 kb on - strand, within SO0429 at 453.727 kb on - strand, within SO0429 at 453.819 kb on + strand at 453.853 kb on + strand at 453.861 kb on - strand at 453.952 kb on - strand at 453.958 kb on - strand at 453.988 kb on - strand at 454.066 kb on - strand at 454.066 kb on - strand at 454.220 kb on - strand at 454.220 kb on - strand at 454.340 kb on - strand at 454.397 kb on - strand, within SO0430 at 454.614 kb on + strand, within SO0430 at 454.636 kb on - strand at 454.774 kb on + strand, within SO0431 at 454.784 kb on + strand, within SO0431 at 454.792 kb on - strand, within SO0431 at 454.916 kb on - strand, within SO0431 at 454.974 kb on + strand, within SO0431 at 454.974 kb on + strand, within SO0431 at 454.982 kb on - strand, within SO0431 at 454.982 kb on - strand, within SO0431 at 454.982 kb on - strand, within SO0431 at 454.992 kb on - strand, within SO0431 at 454.994 kb on + strand, within SO0431 at 454.994 kb on + strand, within SO0431 at 455.096 kb on - strand, within SO0431 at 455.160 kb on - strand, within SO0431 at 455.162 kb on + strand, within SO0431 at 455.162 kb on + strand, within SO0431 at 455.170 kb on - strand, within SO0431 at 455.286 kb on + strand, within SO0431 at 455.399 kb on + strand at 455.405 kb on + strand at 455.405 kb on + strand at 455.412 kb on - strand at 455.413 kb on - strand at 455.435 kb on + strand at 455.435 kb on - strand at 455.606 kb on + strand at 455.640 kb on + strand at 455.650 kb on + strand at 455.668 kb on + strand at 455.671 kb on + strand at 455.682 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M1 center remove 453,727 - SO0429 0.86 -1.4 453,727 - SO0429 0.86 +0.4 453,819 + -1.6 453,853 + +0.1 453,861 - -0.7 453,952 - -1.7 453,958 - +0.1 453,988 - +0.7 454,066 - -1.3 454,066 - +0.3 454,220 - -0.5 454,220 - +0.7 454,340 - -1.6 454,397 - SO0430 0.22 +1.0 454,614 + SO0430 0.88 +1.0 454,636 - -0.1 454,774 + SO0431 0.11 +0.4 454,784 + SO0431 0.12 -0.1 454,792 - SO0431 0.13 -1.6 454,916 - SO0431 0.32 +0.7 454,974 + SO0431 0.41 +0.3 454,974 + SO0431 0.41 +0.3 454,982 - SO0431 0.42 +0.9 454,982 - SO0431 0.42 -0.4 454,982 - SO0431 0.42 +0.8 454,992 - SO0431 0.44 -1.2 454,994 + SO0431 0.44 +0.1 454,994 + SO0431 0.44 +0.5 455,096 - SO0431 0.60 -1.7 455,160 - SO0431 0.70 -1.0 455,162 + SO0431 0.70 -0.7 455,162 + SO0431 0.70 +0.2 455,170 - SO0431 0.71 -1.3 455,286 + SO0431 0.89 -1.0 455,399 + -1.5 455,405 + +0.4 455,405 + +0.9 455,412 - -0.2 455,413 - +0.1 455,435 + -0.1 455,435 - +1.1 455,606 + -0.8 455,640 + +0.0 455,650 + +2.2 455,668 + -1.1 455,671 + +0.6 455,682 - -0.3
Or see this region's nucleotide sequence