Experiment: M1 center
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyQ and tag are separated by 117 nucleotides tag and SO0017 are separated by 86 nucleotides SO0017 and SO0018 are separated by 239 nucleotides SO0018 and SO0019 are separated by 47 nucleotides
SO0015: glyQ - glycyl-tRNA synthetase, alpha subunit (NCBI ptt file), at 17,256 to 18,161
glyQ
SO0016: tag - DNA-3-methyladenine glycosidase I (NCBI ptt file), at 18,279 to 18,854
tag
SO0017: SO0017 - hypothetical amidohydrolase (NCBI ptt file), at 18,941 to 22,090
SO0017
SO0018: SO0018 - conserved hypothetical protein (NCBI ptt file), at 22,330 to 22,767
SO0018
SO0019: SO0019 - conserved hypothetical protein (NCBI ptt file), at 22,815 to 23,060
SO0019
Position (kb)
18
19
20
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 18.212 kb on - strand at 18.270 kb on - strand at 18.386 kb on + strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.394 kb on - strand, within tag at 18.397 kb on - strand, within tag at 18.399 kb on - strand, within tag at 18.411 kb on + strand, within tag at 18.415 kb on - strand, within tag at 18.419 kb on - strand, within tag at 18.488 kb on - strand, within tag at 18.508 kb on + strand, within tag at 18.511 kb on - strand, within tag at 18.518 kb on + strand, within tag at 18.560 kb on + strand, within tag at 18.587 kb on + strand, within tag at 18.602 kb on + strand, within tag at 18.686 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.718 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.775 kb on + strand, within tag at 18.893 kb on + strand at 18.901 kb on - strand at 18.901 kb on - strand at 18.939 kb on + strand at 18.956 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.963 kb on + strand at 18.971 kb on - strand at 19.016 kb on - strand at 19.016 kb on - strand at 19.034 kb on + strand at 19.166 kb on - strand at 19.202 kb on + strand at 19.235 kb on + strand at 19.236 kb on + strand at 19.242 kb on + strand at 19.242 kb on + strand at 19.242 kb on + strand at 19.243 kb on - strand at 19.243 kb on - strand at 19.250 kb on - strand at 19.260 kb on - strand, within SO0017 at 19.323 kb on - strand, within SO0017 at 19.352 kb on - strand, within SO0017 at 19.411 kb on - strand, within SO0017 at 19.461 kb on - strand, within SO0017 at 19.471 kb on + strand, within SO0017 at 19.483 kb on + strand, within SO0017 at 19.506 kb on + strand, within SO0017 at 19.514 kb on - strand, within SO0017 at 19.514 kb on - strand, within SO0017 at 19.514 kb on - strand, within SO0017 at 19.545 kb on + strand, within SO0017 at 19.562 kb on + strand, within SO0017 at 19.571 kb on - strand, within SO0017 at 19.653 kb on + strand, within SO0017 at 19.697 kb on + strand, within SO0017 at 19.705 kb on - strand, within SO0017 at 19.771 kb on - strand, within SO0017 at 19.781 kb on - strand, within SO0017 at 19.794 kb on - strand, within SO0017 at 19.838 kb on + strand, within SO0017 at 19.839 kb on + strand, within SO0017 at 19.863 kb on - strand, within SO0017 at 19.863 kb on - strand, within SO0017 at 19.864 kb on - strand, within SO0017 at 19.924 kb on + strand, within SO0017 at 19.924 kb on + strand, within SO0017 at 19.932 kb on - strand, within SO0017 at 19.932 kb on - strand, within SO0017 at 19.932 kb on - strand, within SO0017 at 19.966 kb on - strand, within SO0017 at 19.978 kb on - strand, within SO0017 at 19.988 kb on - strand, within SO0017 at 19.993 kb on - strand, within SO0017 at 20.114 kb on - strand, within SO0017 at 20.153 kb on + strand, within SO0017 at 20.273 kb on + strand, within SO0017 at 20.278 kb on + strand, within SO0017 at 20.281 kb on - strand, within SO0017 at 20.286 kb on - strand, within SO0017 at 20.286 kb on - strand, within SO0017 at 20.286 kb on - strand, within SO0017 at 20.450 kb on + strand, within SO0017 at 20.455 kb on + strand, within SO0017 at 20.484 kb on - strand, within SO0017 at 20.501 kb on + strand, within SO0017 at 20.526 kb on - strand, within SO0017 at 20.604 kb on + strand, within SO0017 at 20.740 kb on + strand, within SO0017 at 20.767 kb on - strand, within SO0017 at 20.787 kb on + strand, within SO0017 at 20.812 kb on + strand, within SO0017 at 20.861 kb on + strand, within SO0017 at 20.866 kb on + strand, within SO0017 at 21.018 kb on + strand, within SO0017 at 21.047 kb on + strand, within SO0017 at 21.047 kb on + strand, within SO0017 at 21.078 kb on - strand, within SO0017 at 21.089 kb on - strand, within SO0017 at 21.192 kb on + strand, within SO0017 at 21.204 kb on + strand, within SO0017 at 21.212 kb on - strand, within SO0017 at 21.285 kb on - strand, within SO0017 at 21.336 kb on + strand, within SO0017 at 21.341 kb on + strand, within SO0017 at 21.342 kb on + strand, within SO0017 at 21.342 kb on + strand, within SO0017 at 21.352 kb on + strand, within SO0017 at 21.355 kb on - strand, within SO0017 at 21.360 kb on - strand, within SO0017 at 21.360 kb on - strand, within SO0017 at 21.361 kb on + strand, within SO0017 at 21.361 kb on + strand, within SO0017 at 21.370 kb on - strand, within SO0017 at 21.374 kb on - strand, within SO0017 at 21.386 kb on + strand, within SO0017 at 21.441 kb on + strand, within SO0017 at 21.597 kb on - strand, within SO0017 at 21.645 kb on - strand, within SO0017 at 21.695 kb on + strand, within SO0017 at 21.700 kb on + strand, within SO0017 at 21.726 kb on - strand, within SO0017 at 21.823 kb on - strand at 21.823 kb on - strand at 21.838 kb on - strand at 21.865 kb on + strand at 22.075 kb on + strand at 22.083 kb on - strand at 22.098 kb on + strand at 22.114 kb on + strand at 22.114 kb on + strand at 22.119 kb on + strand at 22.122 kb on - strand at 22.127 kb on - strand at 22.305 kb on + strand at 22.313 kb on - strand at 22.385 kb on - strand, within SO0018 at 22.386 kb on + strand, within SO0018 at 22.387 kb on + strand, within SO0018 at 22.394 kb on - strand, within SO0018 at 22.404 kb on + strand, within SO0018 at 22.404 kb on + strand, within SO0018 at 22.404 kb on + strand, within SO0018 at 22.404 kb on + strand, within SO0018 at 22.412 kb on - strand, within SO0018 at 22.412 kb on - strand, within SO0018 at 22.423 kb on + strand, within SO0018 at 22.576 kb on + strand, within SO0018 at 22.576 kb on + strand, within SO0018 at 22.676 kb on + strand, within SO0018 at 22.679 kb on + strand, within SO0018 at 22.679 kb on + strand, within SO0018 at 22.679 kb on + strand, within SO0018 at 22.687 kb on - strand, within SO0018 at 22.687 kb on - strand, within SO0018 at 22.687 kb on - strand, within SO0018 at 22.708 kb on - strand, within SO0018 at 22.710 kb on + strand, within SO0018 at 22.713 kb on - strand, within SO0018 at 22.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M1 center remove 18,212 - -0.5 18,270 - +1.4 18,386 + tag SO0016 0.19 -1.9 18,394 - tag SO0016 0.20 -1.8 18,394 - tag SO0016 0.20 +1.3 18,394 - tag SO0016 0.20 -2.0 18,394 - tag SO0016 0.20 +0.5 18,394 - tag SO0016 0.20 -2.4 18,397 - tag SO0016 0.20 +0.8 18,399 - tag SO0016 0.21 +0.4 18,411 + tag SO0016 0.23 +1.5 18,415 - tag SO0016 0.24 +0.3 18,419 - tag SO0016 0.24 +0.9 18,488 - tag SO0016 0.36 -0.5 18,508 + tag SO0016 0.40 +0.2 18,511 - tag SO0016 0.40 +0.3 18,518 + tag SO0016 0.41 +1.1 18,560 + tag SO0016 0.49 +0.2 18,587 + tag SO0016 0.53 +0.9 18,602 + tag SO0016 0.56 -1.3 18,686 + tag SO0016 0.71 -0.6 18,718 + tag SO0016 0.76 -0.0 18,718 + tag SO0016 0.76 +1.3 18,718 + tag SO0016 0.76 +0.5 18,775 + tag SO0016 0.86 +1.1 18,775 + tag SO0016 0.86 +1.1 18,893 + -1.5 18,901 - -2.0 18,901 - +0.3 18,939 + +0.2 18,956 + -1.0 18,963 + -1.4 18,963 + -2.7 18,963 + +1.9 18,963 + +0.1 18,963 + +0.9 18,971 - -0.8 19,016 - -0.5 19,016 - -0.8 19,034 + -0.5 19,166 - +0.4 19,202 + +0.3 19,235 + -1.8 19,236 + +1.4 19,242 + -0.6 19,242 + -0.3 19,242 + +0.2 19,243 - +0.8 19,243 - +1.5 19,250 - -0.9 19,260 - SO0017 0.10 +0.9 19,323 - SO0017 0.12 -0.1 19,352 - SO0017 0.13 -0.0 19,411 - SO0017 0.15 +0.4 19,461 - SO0017 0.17 -0.1 19,471 + SO0017 0.17 +0.3 19,483 + SO0017 0.17 +2.0 19,506 + SO0017 0.18 +0.3 19,514 - SO0017 0.18 +1.2 19,514 - SO0017 0.18 +0.0 19,514 - SO0017 0.18 -0.7 19,545 + SO0017 0.19 +1.3 19,562 + SO0017 0.20 +0.1 19,571 - SO0017 0.20 +1.1 19,653 + SO0017 0.23 +1.3 19,697 + SO0017 0.24 -0.8 19,705 - SO0017 0.24 +0.1 19,771 - SO0017 0.26 +1.2 19,781 - SO0017 0.27 -2.5 19,794 - SO0017 0.27 -0.8 19,838 + SO0017 0.28 +0.3 19,839 + SO0017 0.29 -1.5 19,863 - SO0017 0.29 -1.5 19,863 - SO0017 0.29 +0.6 19,864 - SO0017 0.29 -1.1 19,924 + SO0017 0.31 +1.7 19,924 + SO0017 0.31 +0.6 19,932 - SO0017 0.31 +0.2 19,932 - SO0017 0.31 -0.6 19,932 - SO0017 0.31 +0.8 19,966 - SO0017 0.33 +0.9 19,978 - SO0017 0.33 -1.5 19,988 - SO0017 0.33 -2.9 19,993 - SO0017 0.33 +1.5 20,114 - SO0017 0.37 -1.4 20,153 + SO0017 0.38 +1.4 20,273 + SO0017 0.42 -0.7 20,278 + SO0017 0.42 -0.3 20,281 - SO0017 0.43 -2.1 20,286 - SO0017 0.43 +0.7 20,286 - SO0017 0.43 +0.2 20,286 - SO0017 0.43 -0.4 20,450 + SO0017 0.48 +0.5 20,455 + SO0017 0.48 +1.0 20,484 - SO0017 0.49 -1.7 20,501 + SO0017 0.50 -0.1 20,526 - SO0017 0.50 -0.9 20,604 + SO0017 0.53 +0.9 20,740 + SO0017 0.57 -0.6 20,767 - SO0017 0.58 -1.5 20,787 + SO0017 0.59 +0.7 20,812 + SO0017 0.59 -0.7 20,861 + SO0017 0.61 +1.1 20,866 + SO0017 0.61 +1.8 21,018 + SO0017 0.66 -1.5 21,047 + SO0017 0.67 -0.8 21,047 + SO0017 0.67 +0.7 21,078 - SO0017 0.68 -0.7 21,089 - SO0017 0.68 -0.8 21,192 + SO0017 0.71 -0.6 21,204 + SO0017 0.72 +3.1 21,212 - SO0017 0.72 -0.7 21,285 - SO0017 0.74 -1.4 21,336 + SO0017 0.76 +1.1 21,341 + SO0017 0.76 -1.8 21,342 + SO0017 0.76 +0.5 21,342 + SO0017 0.76 +1.3 21,352 + SO0017 0.77 -2.7 21,355 - SO0017 0.77 +2.0 21,360 - SO0017 0.77 -0.0 21,360 - SO0017 0.77 +0.2 21,361 + SO0017 0.77 -1.8 21,361 + SO0017 0.77 +0.8 21,370 - SO0017 0.77 -0.7 21,374 - SO0017 0.77 +0.9 21,386 + SO0017 0.78 +3.2 21,441 + SO0017 0.79 +1.2 21,597 - SO0017 0.84 +0.4 21,645 - SO0017 0.86 +3.9 21,695 + SO0017 0.87 +0.9 21,700 + SO0017 0.88 -1.3 21,726 - SO0017 0.88 -0.0 21,823 - -0.7 21,823 - +1.3 21,838 - -0.5 21,865 + -1.7 22,075 + +0.8 22,083 - -3.7 22,098 + +2.1 22,114 + -1.7 22,114 + -1.1 22,119 + -0.1 22,122 - +1.3 22,127 - -1.7 22,305 + -0.1 22,313 - -0.0 22,385 - SO0018 0.13 +0.2 22,386 + SO0018 0.13 -0.4 22,387 + SO0018 0.13 +1.5 22,394 - SO0018 0.15 -0.3 22,404 + SO0018 0.17 -1.0 22,404 + SO0018 0.17 +0.9 22,404 + SO0018 0.17 +2.4 22,404 + SO0018 0.17 -0.5 22,412 - SO0018 0.19 -2.3 22,412 - SO0018 0.19 -0.8 22,423 + SO0018 0.21 +1.0 22,576 + SO0018 0.56 -1.3 22,576 + SO0018 0.56 +2.9 22,676 + SO0018 0.79 +1.3 22,679 + SO0018 0.80 -0.6 22,679 + SO0018 0.80 +0.7 22,679 + SO0018 0.80 -0.3 22,687 - SO0018 0.82 -0.6 22,687 - SO0018 0.82 -0.3 22,687 - SO0018 0.82 -0.4 22,708 - SO0018 0.86 -0.3 22,710 + SO0018 0.87 -1.8 22,713 - SO0018 0.87 +0.6 22,808 - +0.0
Or see this region's nucleotide sequence