Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt topB and SO3062 are separated by 199 nucleotides SO3062 and SO3063 are separated by 293 nucleotides
SO3061: topB - DNA topoisomerase III (NCBI ptt file), at 3,175,740 to 3,177,848
topB
SO3062: SO3062 - hypothetical protein (NCBI ptt file), at 3,178,048 to 3,178,335
SO3062
SO3063: SO3063 - sodium:alanine symporter family protein (NCBI ptt file), at 3,178,629 to 3,180,116
SO3063
Position (kb)
3178
3179 Strain fitness (log2 ratio)
-2
-1
0
1 at 3177.238 kb on - strand, within topB at 3177.307 kb on + strand, within topB at 3177.307 kb on + strand, within topB at 3177.427 kb on + strand, within topB at 3177.557 kb on + strand, within topB at 3177.567 kb on - strand, within topB at 3177.735 kb on - strand at 3177.903 kb on - strand at 3177.969 kb on - strand at 3178.041 kb on - strand at 3178.151 kb on + strand, within SO3062 at 3178.159 kb on - strand, within SO3062 at 3178.247 kb on + strand, within SO3062 at 3178.388 kb on + strand at 3178.411 kb on + strand at 3178.500 kb on + strand at 3178.531 kb on + strand at 3178.660 kb on - strand at 3178.818 kb on + strand, within SO3063 at 3178.818 kb on + strand, within SO3063 at 3178.831 kb on - strand, within SO3063 at 3178.956 kb on + strand, within SO3063 at 3178.962 kb on + strand, within SO3063 at 3179.004 kb on + strand, within SO3063 at 3179.103 kb on + strand, within SO3063 at 3179.160 kb on - strand, within SO3063 at 3179.261 kb on + strand, within SO3063
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 3,177,238 - topB SO3061 0.71 -0.2 3,177,307 + topB SO3061 0.74 -0.2 3,177,307 + topB SO3061 0.74 +0.4 3,177,427 + topB SO3061 0.80 -0.5 3,177,557 + topB SO3061 0.86 +0.7 3,177,567 - topB SO3061 0.87 -0.4 3,177,735 - -0.4 3,177,903 - -0.1 3,177,969 - +0.6 3,178,041 - +0.9 3,178,151 + SO3062 0.36 -1.2 3,178,159 - SO3062 0.39 -0.5 3,178,247 + SO3062 0.69 -2.1 3,178,388 + -0.2 3,178,411 + +0.0 3,178,500 + -0.3 3,178,531 + -0.2 3,178,660 - -0.4 3,178,818 + SO3063 0.13 -0.8 3,178,818 + SO3063 0.13 -1.0 3,178,831 - SO3063 0.14 +0.7 3,178,956 + SO3063 0.22 +1.3 3,178,962 + SO3063 0.22 +0.1 3,179,004 + SO3063 0.25 -0.1 3,179,103 + SO3063 0.32 +0.8 3,179,160 - SO3063 0.36 +0.3 3,179,261 + SO3063 0.42 +0.0
Or see this region's nucleotide sequence