Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 85 nucleotides ruvA and ruvC overlap by 4 nucleotides ruvC and SO2432 are separated by 188 nucleotides
SO2429: ruvB - Holliday junction DNA helicase RuvB (NCBI ptt file), at 2,538,186 to 2,539,190
ruvB
SO2430: ruvA - Holliday junction DNA helicase RuvA (NCBI ptt file), at 2,539,276 to 2,539,893
ruvA
SO2431: ruvC - crossover junction endodeoxyribonuclease RuvC (NCBI ptt file), at 2,539,890 to 2,540,411
ruvC
SO2432: SO2432 - conserved hypothetical protein TIGR01033 (NCBI ptt file), at 2,540,600 to 2,541,346
SO2432
Position (kb)
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2538.446 kb on + strand, within ruvB at 2538.619 kb on - strand, within ruvB at 2538.695 kb on - strand, within ruvB at 2539.094 kb on - strand at 2539.131 kb on + strand at 2539.233 kb on - strand at 2539.326 kb on - strand at 2539.326 kb on - strand at 2539.405 kb on + strand, within ruvA at 2539.481 kb on - strand, within ruvA at 2539.503 kb on - strand, within ruvA at 2539.584 kb on + strand, within ruvA at 2539.886 kb on + strand at 2539.891 kb on + strand at 2539.964 kb on - strand, within ruvC at 2540.395 kb on - strand at 2540.475 kb on + strand at 2540.483 kb on - strand at 2540.570 kb on - strand at 2540.855 kb on - strand, within SO2432
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 2,538,446 + ruvB SO2429 0.26 -0.8 2,538,619 - ruvB SO2429 0.43 -0.7 2,538,695 - ruvB SO2429 0.51 -0.1 2,539,094 - -0.6 2,539,131 + -0.4 2,539,233 - +0.0 2,539,326 - -0.0 2,539,326 - -0.7 2,539,405 + ruvA SO2430 0.21 +0.1 2,539,481 - ruvA SO2430 0.33 -0.6 2,539,503 - ruvA SO2430 0.37 -0.4 2,539,584 + ruvA SO2430 0.50 -1.5 2,539,886 + -3.1 2,539,891 + -2.1 2,539,964 - ruvC SO2431 0.14 -2.0 2,540,395 - -1.3 2,540,475 + -2.5 2,540,483 - +0.1 2,540,570 - +0.4 2,540,855 - SO2432 0.34 +0.4
Or see this region's nucleotide sequence