Experiment: Minimal 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1298.4 and hemL are separated by 922 nucleotides hemL and pyrB are separated by 26 nucleotides
SO1298.4: SO1298.4 - hypothetical Na+/H+ antiporter (NCBI ptt file), at 1,350,495 to 1,351,079
SO1298.4
SO1300: hemL - glutamate-1-semialdehyde-2,1-aminomutase (NCBI ptt file), at 1,352,002 to 1,353,294
hemL
SO1301: pyrB - aspartate carbamoyltransferase (NCBI ptt file), at 1,353,321 to 1,354,340
pyrB
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1351.070 kb on - strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.075 kb on + strand at 1351.083 kb on - strand at 1351.083 kb on - strand at 1351.155 kb on + strand at 1351.247 kb on + strand at 1351.255 kb on - strand at 1351.262 kb on + strand at 1351.267 kb on - strand at 1351.313 kb on + strand at 1351.316 kb on - strand at 1351.326 kb on - strand at 1351.336 kb on + strand at 1351.572 kb on - strand at 1351.583 kb on - strand at 1351.610 kb on - strand at 1351.615 kb on + strand at 1351.659 kb on - strand at 1351.776 kb on + strand at 1351.784 kb on + strand at 1351.847 kb on - strand at 1351.854 kb on - strand at 1353.380 kb on + strand at 1353.407 kb on + strand at 1353.510 kb on - strand, within pyrB at 1353.533 kb on - strand, within pyrB at 1353.543 kb on - strand, within pyrB at 1353.673 kb on + strand, within pyrB at 1353.767 kb on + strand, within pyrB at 1353.775 kb on - strand, within pyrB at 1353.775 kb on - strand, within pyrB at 1353.885 kb on + strand, within pyrB at 1353.998 kb on - strand, within pyrB at 1354.012 kb on + strand, within pyrB at 1354.098 kb on + strand, within pyrB at 1354.113 kb on - strand, within pyrB at 1354.137 kb on + strand, within pyrB at 1354.183 kb on + strand, within pyrB at 1354.227 kb on - strand, within pyrB at 1354.240 kb on + strand at 1354.246 kb on - strand at 1354.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal 30C remove 1,351,070 - +0.7 1,351,075 + -0.2 1,351,075 + -0.1 1,351,075 + +1.3 1,351,075 + -0.3 1,351,075 + -0.5 1,351,083 - -0.1 1,351,083 - -0.4 1,351,155 + +0.3 1,351,247 + -0.6 1,351,255 - +1.8 1,351,262 + +1.0 1,351,267 - +0.1 1,351,313 + -2.4 1,351,316 - -0.1 1,351,326 - -0.0 1,351,336 + +0.3 1,351,572 - -0.2 1,351,583 - -0.1 1,351,610 - -0.5 1,351,615 + +1.3 1,351,659 - -0.0 1,351,776 + +0.4 1,351,784 + -0.2 1,351,847 - -0.2 1,351,854 - -0.1 1,353,380 + -2.0 1,353,407 + -1.5 1,353,510 - pyrB SO1301 0.19 -3.4 1,353,533 - pyrB SO1301 0.21 -3.2 1,353,543 - pyrB SO1301 0.22 -4.1 1,353,673 + pyrB SO1301 0.35 -1.3 1,353,767 + pyrB SO1301 0.44 -2.9 1,353,775 - pyrB SO1301 0.45 -4.1 1,353,775 - pyrB SO1301 0.45 -2.1 1,353,885 + pyrB SO1301 0.55 -2.8 1,353,998 - pyrB SO1301 0.66 -1.0 1,354,012 + pyrB SO1301 0.68 -4.4 1,354,098 + pyrB SO1301 0.76 -4.6 1,354,113 - pyrB SO1301 0.78 -0.7 1,354,137 + pyrB SO1301 0.80 -1.9 1,354,183 + pyrB SO1301 0.85 -1.3 1,354,227 - pyrB SO1301 0.89 -4.5 1,354,240 + -2.9 1,354,246 - -3.0 1,354,248 - -1.8
Or see this region's nucleotide sequence