Experiment: m.b. Choline Lysine 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05280 and PGA1_c05290 are separated by 33 nucleotides PGA1_c05290 and PGA1_c05300 are separated by 144 nucleotides
PGA1_c05280: PGA1_c05280 - hypothetical protein, at 523,673 to 524,959
_c05280
PGA1_c05290: PGA1_c05290 - polysaccharide biosynthesis protein ExoT-like protein, at 524,993 to 526,357
_c05290
PGA1_c05300: PGA1_c05300 - putative glycosyl transferase, at 526,502 to 527,719
_c05300
Position (kb)
524
525
526
527 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 524.113 kb on + strand, within PGA1_c05280 at 524.192 kb on + strand, within PGA1_c05280 at 524.192 kb on + strand, within PGA1_c05280 at 524.193 kb on + strand, within PGA1_c05280 at 524.200 kb on - strand, within PGA1_c05280 at 524.220 kb on - strand, within PGA1_c05280 at 524.235 kb on + strand, within PGA1_c05280 at 524.246 kb on - strand, within PGA1_c05280 at 524.288 kb on + strand, within PGA1_c05280 at 524.288 kb on + strand, within PGA1_c05280 at 524.309 kb on - strand, within PGA1_c05280 at 524.347 kb on + strand, within PGA1_c05280 at 524.355 kb on - strand, within PGA1_c05280 at 524.355 kb on - strand, within PGA1_c05280 at 524.400 kb on + strand, within PGA1_c05280 at 524.400 kb on + strand, within PGA1_c05280 at 524.410 kb on + strand, within PGA1_c05280 at 524.436 kb on - strand, within PGA1_c05280 at 524.463 kb on + strand, within PGA1_c05280 at 524.464 kb on + strand, within PGA1_c05280 at 524.571 kb on - strand, within PGA1_c05280 at 524.578 kb on + strand, within PGA1_c05280 at 524.578 kb on + strand, within PGA1_c05280 at 524.730 kb on - strand, within PGA1_c05280 at 524.762 kb on + strand, within PGA1_c05280 at 524.811 kb on + strand, within PGA1_c05280 at 524.819 kb on - strand, within PGA1_c05280 at 524.867 kb on + strand at 524.867 kb on + strand at 524.875 kb on - strand at 524.935 kb on + strand at 524.940 kb on + strand at 524.974 kb on + strand at 524.982 kb on - strand at 524.997 kb on - strand at 524.997 kb on - strand at 525.060 kb on - strand at 525.083 kb on - strand at 525.151 kb on - strand, within PGA1_c05290 at 525.151 kb on - strand, within PGA1_c05290 at 525.190 kb on - strand, within PGA1_c05290 at 525.221 kb on + strand, within PGA1_c05290 at 525.269 kb on + strand, within PGA1_c05290 at 525.269 kb on + strand, within PGA1_c05290 at 525.300 kb on - strand, within PGA1_c05290 at 525.307 kb on - strand, within PGA1_c05290 at 525.343 kb on + strand, within PGA1_c05290 at 525.346 kb on + strand, within PGA1_c05290 at 525.346 kb on + strand, within PGA1_c05290 at 525.356 kb on + strand, within PGA1_c05290 at 525.375 kb on + strand, within PGA1_c05290 at 525.383 kb on - strand, within PGA1_c05290 at 525.394 kb on + strand, within PGA1_c05290 at 525.408 kb on - strand, within PGA1_c05290 at 525.448 kb on + strand, within PGA1_c05290 at 525.471 kb on - strand, within PGA1_c05290 at 525.479 kb on + strand, within PGA1_c05290 at 525.479 kb on + strand, within PGA1_c05290 at 525.481 kb on + strand, within PGA1_c05290 at 525.487 kb on - strand, within PGA1_c05290 at 525.487 kb on - strand, within PGA1_c05290 at 525.487 kb on - strand, within PGA1_c05290 at 525.487 kb on - strand, within PGA1_c05290 at 525.487 kb on - strand, within PGA1_c05290 at 525.496 kb on + strand, within PGA1_c05290 at 525.501 kb on + strand, within PGA1_c05290 at 525.501 kb on + strand, within PGA1_c05290 at 525.501 kb on + strand, within PGA1_c05290 at 525.503 kb on - strand, within PGA1_c05290 at 525.509 kb on - strand, within PGA1_c05290 at 525.541 kb on + strand, within PGA1_c05290 at 525.548 kb on + strand, within PGA1_c05290 at 525.549 kb on - strand, within PGA1_c05290 at 525.556 kb on - strand, within PGA1_c05290 at 525.596 kb on + strand, within PGA1_c05290 at 525.613 kb on + strand, within PGA1_c05290 at 525.626 kb on - strand, within PGA1_c05290 at 525.628 kb on + strand, within PGA1_c05290 at 525.674 kb on - strand, within PGA1_c05290 at 525.712 kb on - strand, within PGA1_c05290 at 525.754 kb on + strand, within PGA1_c05290 at 525.762 kb on - strand, within PGA1_c05290 at 525.796 kb on - strand, within PGA1_c05290 at 525.798 kb on - strand, within PGA1_c05290 at 525.836 kb on + strand, within PGA1_c05290 at 525.844 kb on - strand, within PGA1_c05290 at 525.888 kb on - strand, within PGA1_c05290 at 525.924 kb on + strand, within PGA1_c05290 at 525.940 kb on + strand, within PGA1_c05290 at 525.943 kb on - strand, within PGA1_c05290 at 525.953 kb on - strand, within PGA1_c05290 at 525.984 kb on + strand, within PGA1_c05290 at 525.984 kb on + strand, within PGA1_c05290 at 525.986 kb on + strand, within PGA1_c05290 at 525.992 kb on - strand, within PGA1_c05290 at 525.992 kb on - strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.036 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.062 kb on - strand, within PGA1_c05290 at 526.080 kb on - strand, within PGA1_c05290 at 526.116 kb on + strand, within PGA1_c05290 at 526.116 kb on + strand, within PGA1_c05290 at 526.182 kb on + strand, within PGA1_c05290 at 526.185 kb on - strand, within PGA1_c05290 at 526.187 kb on + strand, within PGA1_c05290 at 526.190 kb on - strand, within PGA1_c05290 at 526.285 kb on - strand at 526.304 kb on - strand at 526.330 kb on - strand at 526.346 kb on + strand at 526.390 kb on + strand at 526.437 kb on - strand at 526.532 kb on - strand at 526.535 kb on - strand at 526.573 kb on - strand at 526.579 kb on - strand at 526.593 kb on - strand at 526.660 kb on + strand, within PGA1_c05300 at 526.660 kb on + strand, within PGA1_c05300 at 526.663 kb on - strand, within PGA1_c05300 at 526.663 kb on - strand, within PGA1_c05300 at 526.668 kb on + strand, within PGA1_c05300 at 526.671 kb on + strand, within PGA1_c05300 at 526.752 kb on - strand, within PGA1_c05300 at 526.759 kb on + strand, within PGA1_c05300 at 526.925 kb on + strand, within PGA1_c05300 at 526.932 kb on - strand, within PGA1_c05300 at 526.939 kb on + strand, within PGA1_c05300 at 526.988 kb on - strand, within PGA1_c05300 at 526.988 kb on - strand, within PGA1_c05300 at 527.083 kb on - strand, within PGA1_c05300 at 527.085 kb on + strand, within PGA1_c05300 at 527.093 kb on + strand, within PGA1_c05300 at 527.115 kb on + strand, within PGA1_c05300 at 527.124 kb on - strand, within PGA1_c05300 at 527.154 kb on - strand, within PGA1_c05300 at 527.226 kb on - strand, within PGA1_c05300 at 527.250 kb on + strand, within PGA1_c05300 at 527.250 kb on + strand, within PGA1_c05300 at 527.258 kb on - strand, within PGA1_c05300 at 527.277 kb on + strand, within PGA1_c05300 at 527.320 kb on + strand, within PGA1_c05300
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Choline Lysine 3 mM remove 524,113 + PGA1_c05280 0.34 -0.2 524,192 + PGA1_c05280 0.40 +0.6 524,192 + PGA1_c05280 0.40 -0.4 524,193 + PGA1_c05280 0.40 +0.6 524,200 - PGA1_c05280 0.41 +0.8 524,220 - PGA1_c05280 0.43 -0.6 524,235 + PGA1_c05280 0.44 +0.6 524,246 - PGA1_c05280 0.45 +0.7 524,288 + PGA1_c05280 0.48 -0.7 524,288 + PGA1_c05280 0.48 +0.2 524,309 - PGA1_c05280 0.49 +0.3 524,347 + PGA1_c05280 0.52 -0.5 524,355 - PGA1_c05280 0.53 -0.0 524,355 - PGA1_c05280 0.53 +1.1 524,400 + PGA1_c05280 0.56 -0.3 524,400 + PGA1_c05280 0.56 +0.8 524,410 + PGA1_c05280 0.57 +0.1 524,436 - PGA1_c05280 0.59 +2.5 524,463 + PGA1_c05280 0.61 +0.2 524,464 + PGA1_c05280 0.61 +0.5 524,571 - PGA1_c05280 0.70 +0.2 524,578 + PGA1_c05280 0.70 -0.5 524,578 + PGA1_c05280 0.70 +1.1 524,730 - PGA1_c05280 0.82 +0.1 524,762 + PGA1_c05280 0.85 -0.1 524,811 + PGA1_c05280 0.88 -0.1 524,819 - PGA1_c05280 0.89 -1.9 524,867 + -0.7 524,867 + +0.2 524,875 - +1.7 524,935 + -0.6 524,940 + -1.0 524,974 + +0.3 524,982 - -0.1 524,997 - -0.2 524,997 - -0.6 525,060 - +0.7 525,083 - +0.1 525,151 - PGA1_c05290 0.12 +0.5 525,151 - PGA1_c05290 0.12 +0.3 525,190 - PGA1_c05290 0.14 +0.2 525,221 + PGA1_c05290 0.17 -0.6 525,269 + PGA1_c05290 0.20 +0.4 525,269 + PGA1_c05290 0.20 +0.6 525,300 - PGA1_c05290 0.22 -0.1 525,307 - PGA1_c05290 0.23 -0.7 525,343 + PGA1_c05290 0.26 +0.3 525,346 + PGA1_c05290 0.26 +1.4 525,346 + PGA1_c05290 0.26 -0.8 525,356 + PGA1_c05290 0.27 +1.0 525,375 + PGA1_c05290 0.28 +0.4 525,383 - PGA1_c05290 0.29 +1.2 525,394 + PGA1_c05290 0.29 +0.2 525,408 - PGA1_c05290 0.30 +1.5 525,448 + PGA1_c05290 0.33 +0.4 525,471 - PGA1_c05290 0.35 +0.1 525,479 + PGA1_c05290 0.36 +1.2 525,479 + PGA1_c05290 0.36 +1.2 525,481 + PGA1_c05290 0.36 +0.6 525,487 - PGA1_c05290 0.36 +0.6 525,487 - PGA1_c05290 0.36 +0.2 525,487 - PGA1_c05290 0.36 -1.6 525,487 - PGA1_c05290 0.36 -0.1 525,487 - PGA1_c05290 0.36 +0.6 525,496 + PGA1_c05290 0.37 +0.9 525,501 + PGA1_c05290 0.37 +0.6 525,501 + PGA1_c05290 0.37 +0.6 525,501 + PGA1_c05290 0.37 -1.6 525,503 - PGA1_c05290 0.37 +1.0 525,509 - PGA1_c05290 0.38 +0.3 525,541 + PGA1_c05290 0.40 +1.2 525,548 + PGA1_c05290 0.41 +0.7 525,549 - PGA1_c05290 0.41 +0.2 525,556 - PGA1_c05290 0.41 -0.8 525,596 + PGA1_c05290 0.44 +1.2 525,613 + PGA1_c05290 0.45 -0.3 525,626 - PGA1_c05290 0.46 +0.6 525,628 + PGA1_c05290 0.47 +0.8 525,674 - PGA1_c05290 0.50 -2.0 525,712 - PGA1_c05290 0.53 +0.7 525,754 + PGA1_c05290 0.56 -0.8 525,762 - PGA1_c05290 0.56 +0.1 525,796 - PGA1_c05290 0.59 -0.4 525,798 - PGA1_c05290 0.59 +1.2 525,836 + PGA1_c05290 0.62 +0.4 525,844 - PGA1_c05290 0.62 +0.9 525,888 - PGA1_c05290 0.66 -1.0 525,924 + PGA1_c05290 0.68 +0.3 525,940 + PGA1_c05290 0.69 +0.6 525,943 - PGA1_c05290 0.70 -0.3 525,953 - PGA1_c05290 0.70 +0.3 525,984 + PGA1_c05290 0.73 -0.1 525,984 + PGA1_c05290 0.73 +0.2 525,986 + PGA1_c05290 0.73 +1.5 525,992 - PGA1_c05290 0.73 -1.4 525,992 - PGA1_c05290 0.73 -0.3 526,029 + PGA1_c05290 0.76 -1.3 526,029 + PGA1_c05290 0.76 -0.7 526,029 + PGA1_c05290 0.76 +0.3 526,036 - PGA1_c05290 0.76 +0.9 526,037 - PGA1_c05290 0.76 -0.6 526,037 - PGA1_c05290 0.76 -0.7 526,037 - PGA1_c05290 0.76 +1.7 526,062 - PGA1_c05290 0.78 +0.2 526,080 - PGA1_c05290 0.80 -0.5 526,116 + PGA1_c05290 0.82 -0.5 526,116 + PGA1_c05290 0.82 +0.2 526,182 + PGA1_c05290 0.87 +0.2 526,185 - PGA1_c05290 0.87 +0.7 526,187 + PGA1_c05290 0.87 +0.6 526,190 - PGA1_c05290 0.88 +0.1 526,285 - -2.4 526,304 - -0.4 526,330 - -2.4 526,346 + +0.1 526,390 + -0.0 526,437 - +1.0 526,532 - +0.1 526,535 - +0.7 526,573 - +1.5 526,579 - +0.3 526,593 - +1.1 526,660 + PGA1_c05300 0.13 +1.0 526,660 + PGA1_c05300 0.13 +0.2 526,663 - PGA1_c05300 0.13 -0.9 526,663 - PGA1_c05300 0.13 +0.6 526,668 + PGA1_c05300 0.14 -0.8 526,671 + PGA1_c05300 0.14 +0.2 526,752 - PGA1_c05300 0.21 +0.6 526,759 + PGA1_c05300 0.21 -0.2 526,925 + PGA1_c05300 0.35 -0.1 526,932 - PGA1_c05300 0.35 +0.4 526,939 + PGA1_c05300 0.36 +1.4 526,988 - PGA1_c05300 0.40 -0.6 526,988 - PGA1_c05300 0.40 +0.5 527,083 - PGA1_c05300 0.48 -0.7 527,085 + PGA1_c05300 0.48 -1.4 527,093 + PGA1_c05300 0.49 -0.5 527,115 + PGA1_c05300 0.50 +0.8 527,124 - PGA1_c05300 0.51 +0.9 527,154 - PGA1_c05300 0.54 +0.8 527,226 - PGA1_c05300 0.59 +1.6 527,250 + PGA1_c05300 0.61 +0.7 527,250 + PGA1_c05300 0.61 +2.2 527,258 - PGA1_c05300 0.62 -0.0 527,277 + PGA1_c05300 0.64 +0.9 527,320 + PGA1_c05300 0.67 +0.6
Or see this region's nucleotide sequence