Experiment: m.b. Choline Lysine 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30290 and PGA1_c30300 are separated by 183 nucleotides PGA1_c30300 and PGA1_c30310 are separated by 255 nucleotides PGA1_c30310 and PGA1_c30320 are separated by 142 nucleotides
PGA1_c30290: PGA1_c30290 - recombination protein RecR, at 3,167,803 to 3,168,402
_c30290
PGA1_c30300: PGA1_c30300 - tRNA-Met, at 3,168,586 to 3,168,662
_c30300
PGA1_c30310: PGA1_c30310 - Uncharacterized protein conserved in bacteria, at 3,168,918 to 3,169,697
_c30310
PGA1_c30320: PGA1_c30320 - putative ribonuclease, at 3,169,840 to 3,170,505
_c30320
Position (kb)
3168
3169
3170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3167.978 kb on + strand, within PGA1_c30290 at 3167.986 kb on - strand, within PGA1_c30290 at 3167.986 kb on - strand, within PGA1_c30290 at 3168.009 kb on + strand, within PGA1_c30290 at 3168.009 kb on + strand, within PGA1_c30290 at 3168.017 kb on - strand, within PGA1_c30290 at 3168.017 kb on - strand, within PGA1_c30290 at 3168.028 kb on + strand, within PGA1_c30290 at 3168.028 kb on + strand, within PGA1_c30290 at 3168.028 kb on + strand, within PGA1_c30290 at 3168.028 kb on + strand, within PGA1_c30290 at 3168.028 kb on + strand, within PGA1_c30290 at 3168.036 kb on - strand, within PGA1_c30290 at 3168.036 kb on - strand, within PGA1_c30290 at 3168.169 kb on - strand, within PGA1_c30290 at 3168.309 kb on - strand, within PGA1_c30290 at 3168.396 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.423 kb on + strand at 3168.431 kb on - strand at 3168.431 kb on - strand at 3168.431 kb on - strand at 3168.431 kb on - strand at 3168.443 kb on + strand at 3168.446 kb on - strand at 3168.446 kb on - strand at 3168.464 kb on + strand at 3168.464 kb on + strand at 3168.477 kb on - strand at 3168.477 kb on - strand at 3168.524 kb on - strand at 3168.727 kb on + strand at 3168.727 kb on + strand at 3168.773 kb on - strand at 3168.781 kb on + strand at 3168.783 kb on - strand at 3168.783 kb on - strand at 3168.790 kb on - strand at 3168.873 kb on + strand at 3169.060 kb on - strand, within PGA1_c30310 at 3169.083 kb on + strand, within PGA1_c30310 at 3169.084 kb on + strand, within PGA1_c30310 at 3169.089 kb on - strand, within PGA1_c30310 at 3169.091 kb on - strand, within PGA1_c30310 at 3169.123 kb on + strand, within PGA1_c30310 at 3169.131 kb on - strand, within PGA1_c30310 at 3169.847 kb on + strand at 3169.847 kb on + strand at 3169.851 kb on - strand at 3169.855 kb on - strand at 3169.855 kb on - strand at 3169.855 kb on - strand at 3169.888 kb on + strand at 3169.917 kb on - strand, within PGA1_c30320 at 3169.964 kb on + strand, within PGA1_c30320 at 3169.965 kb on - strand, within PGA1_c30320 at 3170.020 kb on - strand, within PGA1_c30320 at 3170.037 kb on - strand, within PGA1_c30320 at 3170.120 kb on - strand, within PGA1_c30320 at 3170.120 kb on - strand, within PGA1_c30320 at 3170.124 kb on - strand, within PGA1_c30320 at 3170.162 kb on + strand, within PGA1_c30320 at 3170.182 kb on - strand, within PGA1_c30320 at 3170.204 kb on + strand, within PGA1_c30320 at 3170.204 kb on + strand, within PGA1_c30320 at 3170.204 kb on + strand, within PGA1_c30320 at 3170.204 kb on + strand, within PGA1_c30320 at 3170.204 kb on + strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.212 kb on - strand, within PGA1_c30320 at 3170.231 kb on - strand, within PGA1_c30320 at 3170.249 kb on + strand, within PGA1_c30320 at 3170.267 kb on - strand, within PGA1_c30320 at 3170.323 kb on - strand, within PGA1_c30320 at 3170.340 kb on + strand, within PGA1_c30320 at 3170.348 kb on - strand, within PGA1_c30320 at 3170.353 kb on - strand, within PGA1_c30320 at 3170.366 kb on - strand, within PGA1_c30320 at 3170.394 kb on + strand, within PGA1_c30320 at 3170.402 kb on - strand, within PGA1_c30320 at 3170.404 kb on + strand, within PGA1_c30320 at 3170.535 kb on - strand at 3170.550 kb on + strand at 3170.559 kb on - strand at 3170.641 kb on + strand at 3170.641 kb on + strand at 3170.689 kb on + strand at 3170.689 kb on + strand at 3170.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Choline Lysine 3 mM remove 3,167,978 + PGA1_c30290 0.29 -0.7 3,167,986 - PGA1_c30290 0.30 -0.4 3,167,986 - PGA1_c30290 0.30 +0.4 3,168,009 + PGA1_c30290 0.34 -0.0 3,168,009 + PGA1_c30290 0.34 -2.4 3,168,017 - PGA1_c30290 0.36 -1.6 3,168,017 - PGA1_c30290 0.36 -0.4 3,168,028 + PGA1_c30290 0.38 -1.3 3,168,028 + PGA1_c30290 0.38 -2.0 3,168,028 + PGA1_c30290 0.38 -2.5 3,168,028 + PGA1_c30290 0.38 +1.6 3,168,028 + PGA1_c30290 0.38 -1.3 3,168,036 - PGA1_c30290 0.39 -0.9 3,168,036 - PGA1_c30290 0.39 -0.1 3,168,169 - PGA1_c30290 0.61 -3.4 3,168,309 - PGA1_c30290 0.84 -0.1 3,168,396 + -0.7 3,168,423 + -0.8 3,168,423 + -1.4 3,168,423 + -0.1 3,168,423 + +0.4 3,168,423 + -0.6 3,168,423 + -0.0 3,168,423 + +0.9 3,168,423 + -0.2 3,168,431 - -0.8 3,168,431 - -0.4 3,168,431 - -1.2 3,168,431 - +0.7 3,168,443 + +0.3 3,168,446 - -0.4 3,168,446 - +0.2 3,168,464 + -0.7 3,168,464 + -0.0 3,168,477 - -2.7 3,168,477 - -0.1 3,168,524 - -0.4 3,168,727 + -0.2 3,168,727 + -1.0 3,168,773 - +0.3 3,168,781 + +0.3 3,168,783 - -0.4 3,168,783 - -1.4 3,168,790 - -0.7 3,168,873 + +0.6 3,169,060 - PGA1_c30310 0.18 +0.1 3,169,083 + PGA1_c30310 0.21 +0.5 3,169,084 + PGA1_c30310 0.21 -1.4 3,169,089 - PGA1_c30310 0.22 -1.4 3,169,091 - PGA1_c30310 0.22 -2.9 3,169,123 + PGA1_c30310 0.26 +2.2 3,169,131 - PGA1_c30310 0.27 -1.7 3,169,847 + +0.2 3,169,847 + -0.4 3,169,851 - -0.3 3,169,855 - +1.5 3,169,855 - +3.2 3,169,855 - -0.6 3,169,888 + -0.3 3,169,917 - PGA1_c30320 0.12 +0.1 3,169,964 + PGA1_c30320 0.19 -0.3 3,169,965 - PGA1_c30320 0.19 +0.2 3,170,020 - PGA1_c30320 0.27 -0.5 3,170,037 - PGA1_c30320 0.30 -1.6 3,170,120 - PGA1_c30320 0.42 -1.1 3,170,120 - PGA1_c30320 0.42 -1.3 3,170,124 - PGA1_c30320 0.43 -0.3 3,170,162 + PGA1_c30320 0.48 +1.4 3,170,182 - PGA1_c30320 0.51 -1.3 3,170,204 + PGA1_c30320 0.55 -2.0 3,170,204 + PGA1_c30320 0.55 +0.1 3,170,204 + PGA1_c30320 0.55 +0.5 3,170,204 + PGA1_c30320 0.55 +0.1 3,170,204 + PGA1_c30320 0.55 -0.6 3,170,212 - PGA1_c30320 0.56 +0.2 3,170,212 - PGA1_c30320 0.56 -0.3 3,170,212 - PGA1_c30320 0.56 +0.2 3,170,212 - PGA1_c30320 0.56 +1.6 3,170,212 - PGA1_c30320 0.56 +0.5 3,170,212 - PGA1_c30320 0.56 +0.8 3,170,212 - PGA1_c30320 0.56 +0.2 3,170,212 - PGA1_c30320 0.56 -1.2 3,170,212 - PGA1_c30320 0.56 -1.2 3,170,212 - PGA1_c30320 0.56 -0.1 3,170,212 - PGA1_c30320 0.56 -1.7 3,170,212 - PGA1_c30320 0.56 -0.5 3,170,212 - PGA1_c30320 0.56 -0.3 3,170,231 - PGA1_c30320 0.59 +0.5 3,170,249 + PGA1_c30320 0.61 -1.0 3,170,267 - PGA1_c30320 0.64 -0.3 3,170,323 - PGA1_c30320 0.73 +0.9 3,170,340 + PGA1_c30320 0.75 +1.6 3,170,348 - PGA1_c30320 0.76 -1.4 3,170,353 - PGA1_c30320 0.77 +0.4 3,170,366 - PGA1_c30320 0.79 -0.0 3,170,394 + PGA1_c30320 0.83 +0.7 3,170,402 - PGA1_c30320 0.84 -0.8 3,170,404 + PGA1_c30320 0.85 +0.2 3,170,535 - +0.6 3,170,550 + -0.2 3,170,559 - +0.7 3,170,641 + -0.1 3,170,641 + +0.5 3,170,689 + +0.4 3,170,689 + -0.1 3,170,697 - +1.1
Or see this region's nucleotide sequence