Experiment: Minimal 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvA and ruvC overlap by 4 nucleotides ruvC and SO2432 are separated by 188 nucleotides SO2432 and aspS are separated by 216 nucleotides
SO2430: ruvA - Holliday junction DNA helicase RuvA (NCBI ptt file), at 2,539,276 to 2,539,893
ruvA
SO2431: ruvC - crossover junction endodeoxyribonuclease RuvC (NCBI ptt file), at 2,539,890 to 2,540,411
ruvC
SO2432: SO2432 - conserved hypothetical protein TIGR01033 (NCBI ptt file), at 2,540,600 to 2,541,346
SO2432
SO2433: aspS - aspartyl-tRNA synthetase (NCBI ptt file), at 2,541,563 to 2,543,338
aspS
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-2
-1
0
1 at 2539.886 kb on + strand at 2539.891 kb on + strand at 2539.964 kb on - strand, within ruvC at 2540.395 kb on - strand at 2540.475 kb on + strand at 2540.483 kb on - strand at 2540.570 kb on - strand at 2540.855 kb on - strand, within SO2432 at 2540.924 kb on + strand, within SO2432 at 2540.980 kb on - strand, within SO2432 at 2541.074 kb on + strand, within SO2432 at 2541.311 kb on - strand at 2541.356 kb on + strand at 2541.362 kb on - strand at 2541.364 kb on - strand at 2541.364 kb on - strand at 2541.364 kb on - strand at 2541.390 kb on + strand at 2541.441 kb on + strand at 2541.441 kb on + strand at 2541.592 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal 20C remove 2,539,886 + -2.2 2,539,891 + -1.2 2,539,964 - ruvC SO2431 0.14 -1.5 2,540,395 - +0.1 2,540,475 + -1.5 2,540,483 - +0.6 2,540,570 - +0.4 2,540,855 - SO2432 0.34 -0.0 2,540,924 + SO2432 0.43 -0.4 2,540,980 - SO2432 0.51 -0.1 2,541,074 + SO2432 0.63 +0.1 2,541,311 - -1.6 2,541,356 + +0.4 2,541,362 - +0.2 2,541,364 - -0.1 2,541,364 - +0.4 2,541,364 - -0.2 2,541,390 + +0.1 2,541,441 + -0.2 2,541,441 + -0.5 2,541,592 + -0.4
Or see this region's nucleotide sequence