Experiment: biofilm; Day2; NLDM; Aluminum chloride 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS04180 and LRK54_RS04185 are separated by 130 nucleotides LRK54_RS04185 and LRK54_RS04190 overlap by 4 nucleotides LRK54_RS04190 and LRK54_RS04195 are separated by 22 nucleotides
LRK54_RS04180: LRK54_RS04180 - methyl-accepting chemotaxis protein, at 885,817 to 887,847
_RS04180
LRK54_RS04185: LRK54_RS04185 - chemotaxis protein CheW, at 887,978 to 888,517
_RS04185
LRK54_RS04190: LRK54_RS04190 - response regulator, at 888,514 to 888,885
_RS04190
LRK54_RS04195: LRK54_RS04195 - twitching motility response regulator PilG, at 888,908 to 889,306
_RS04195
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 887.198 kb on + strand, within LRK54_RS04180 at 887.198 kb on + strand, within LRK54_RS04180 at 887.198 kb on + strand, within LRK54_RS04180 at 887.198 kb on + strand, within LRK54_RS04180 at 887.198 kb on + strand, within LRK54_RS04180 at 887.199 kb on - strand, within LRK54_RS04180 at 887.199 kb on - strand, within LRK54_RS04180 at 887.199 kb on - strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.409 kb on + strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.410 kb on - strand, within LRK54_RS04180 at 887.543 kb on + strand, within LRK54_RS04180 at 887.543 kb on + strand, within LRK54_RS04180 at 887.543 kb on + strand, within LRK54_RS04180 at 887.544 kb on - strand, within LRK54_RS04180 at 887.808 kb on + strand at 887.808 kb on + strand at 887.808 kb on + strand at 887.808 kb on + strand at 887.808 kb on + strand at 887.808 kb on + strand at 887.808 kb on + strand at 887.809 kb on - strand at 887.809 kb on - strand at 887.809 kb on - strand at 887.809 kb on - strand at 887.809 kb on - strand at 887.809 kb on - strand at 887.810 kb on + strand at 887.811 kb on - strand at 887.811 kb on - strand at 887.811 kb on - strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.838 kb on + strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.839 kb on - strand at 887.864 kb on + strand at 887.864 kb on + strand at 887.864 kb on + strand at 887.864 kb on + strand at 887.865 kb on - strand at 887.865 kb on - strand at 887.865 kb on - strand at 887.872 kb on + strand at 887.872 kb on + strand at 887.872 kb on + strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 887.873 kb on - strand at 888.202 kb on + strand, within LRK54_RS04185 at 888.440 kb on - strand, within LRK54_RS04185 at 888.471 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.507 kb on + strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.508 kb on - strand at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.690 kb on + strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.691 kb on - strand, within LRK54_RS04190 at 888.920 kb on + strand at 888.920 kb on + strand at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.984 kb on + strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 888.985 kb on - strand, within LRK54_RS04195 at 889.351 kb on + strand at 889.351 kb on + strand at 889.351 kb on + strand at 889.352 kb on - strand at 889.352 kb on - strand at 889.352 kb on - strand at 889.352 kb on - strand at 889.352 kb on - strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.386 kb on + strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.387 kb on - strand at 889.394 kb on + strand at 889.394 kb on + strand at 889.394 kb on + strand at 889.395 kb on - strand at 889.395 kb on - strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.429 kb on + strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.459 kb on + strand at 889.459 kb on + strand at 889.460 kb on - strand at 889.460 kb on - strand at 889.489 kb on + strand at 889.489 kb on + strand at 889.490 kb on - strand at 889.490 kb on - strand at 889.490 kb on - strand at 889.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; Day2; NLDM; Aluminum chloride 1mM remove 887,198 + LRK54_RS04180 0.68 +1.8 887,198 + LRK54_RS04180 0.68 +1.1 887,198 + LRK54_RS04180 0.68 +0.3 887,198 + LRK54_RS04180 0.68 -1.9 887,198 + LRK54_RS04180 0.68 -0.2 887,199 - LRK54_RS04180 0.68 +1.8 887,199 - LRK54_RS04180 0.68 -0.5 887,199 - LRK54_RS04180 0.68 +1.7 887,409 + LRK54_RS04180 0.78 +0.3 887,409 + LRK54_RS04180 0.78 -2.0 887,409 + LRK54_RS04180 0.78 -0.9 887,409 + LRK54_RS04180 0.78 +3.8 887,409 + LRK54_RS04180 0.78 -0.2 887,409 + LRK54_RS04180 0.78 +0.7 887,410 - LRK54_RS04180 0.78 -0.9 887,410 - LRK54_RS04180 0.78 +0.4 887,410 - LRK54_RS04180 0.78 +0.1 887,410 - LRK54_RS04180 0.78 +0.6 887,410 - LRK54_RS04180 0.78 -1.1 887,410 - LRK54_RS04180 0.78 -1.7 887,543 + LRK54_RS04180 0.85 +1.5 887,543 + LRK54_RS04180 0.85 -0.5 887,543 + LRK54_RS04180 0.85 +1.8 887,544 - LRK54_RS04180 0.85 -2.5 887,808 + -0.1 887,808 + +0.9 887,808 + +0.0 887,808 + +0.6 887,808 + -0.6 887,808 + -1.4 887,808 + +1.7 887,809 - -2.0 887,809 - -0.6 887,809 - +2.8 887,809 - +0.8 887,809 - +1.5 887,809 - +0.1 887,810 + +3.3 887,811 - +1.5 887,811 - +1.7 887,811 - +1.7 887,838 + +0.6 887,838 + +1.9 887,838 + +0.2 887,838 + +1.0 887,838 + +0.1 887,838 + -2.2 887,838 + +3.0 887,838 + +0.1 887,838 + +1.3 887,839 - -0.8 887,839 - +1.7 887,839 - -1.1 887,839 - -1.1 887,839 - +2.1 887,839 - -0.3 887,839 - -0.5 887,839 - +0.8 887,864 + -0.4 887,864 + +0.5 887,864 + -1.2 887,864 + -1.6 887,865 - +0.4 887,865 - +1.0 887,865 - +0.3 887,872 + +0.8 887,872 + -0.2 887,872 + +0.4 887,873 - -1.2 887,873 - -0.1 887,873 - +1.8 887,873 - +0.8 887,873 - +1.1 887,873 - -0.5 887,873 - -1.1 887,873 - -0.7 887,873 - -1.1 888,202 + LRK54_RS04185 0.41 +1.3 888,440 - LRK54_RS04185 0.86 -0.5 888,471 + -0.5 888,507 + -0.2 888,507 + +2.1 888,507 + -0.7 888,507 + +0.7 888,507 + +0.3 888,507 + -0.1 888,507 + +0.8 888,507 + -1.0 888,508 - -2.4 888,508 - -0.5 888,508 - +0.5 888,508 - -1.6 888,508 - -2.0 888,508 - +1.8 888,508 - +1.7 888,690 + LRK54_RS04190 0.47 +1.5 888,690 + LRK54_RS04190 0.47 -0.5 888,690 + LRK54_RS04190 0.47 +1.0 888,690 + LRK54_RS04190 0.47 +1.4 888,690 + LRK54_RS04190 0.47 +0.4 888,690 + LRK54_RS04190 0.47 -0.1 888,690 + LRK54_RS04190 0.47 +1.3 888,691 - LRK54_RS04190 0.48 -1.1 888,691 - LRK54_RS04190 0.48 +0.7 888,691 - LRK54_RS04190 0.48 +0.3 888,691 - LRK54_RS04190 0.48 +1.2 888,691 - LRK54_RS04190 0.48 +0.4 888,691 - LRK54_RS04190 0.48 -1.0 888,691 - LRK54_RS04190 0.48 -0.5 888,691 - LRK54_RS04190 0.48 +0.0 888,920 + +1.7 888,920 + -1.8 888,984 + LRK54_RS04195 0.19 +0.3 888,984 + LRK54_RS04195 0.19 +1.0 888,984 + LRK54_RS04195 0.19 +0.3 888,984 + LRK54_RS04195 0.19 -0.5 888,984 + LRK54_RS04195 0.19 -2.7 888,984 + LRK54_RS04195 0.19 -2.0 888,984 + LRK54_RS04195 0.19 +1.0 888,984 + LRK54_RS04195 0.19 +0.5 888,984 + LRK54_RS04195 0.19 -2.1 888,985 - LRK54_RS04195 0.19 -0.1 888,985 - LRK54_RS04195 0.19 -0.2 888,985 - LRK54_RS04195 0.19 +0.7 888,985 - LRK54_RS04195 0.19 -1.9 888,985 - LRK54_RS04195 0.19 +1.2 888,985 - LRK54_RS04195 0.19 +0.2 888,985 - LRK54_RS04195 0.19 -1.0 888,985 - LRK54_RS04195 0.19 +0.6 888,985 - LRK54_RS04195 0.19 +1.2 888,985 - LRK54_RS04195 0.19 +0.9 888,985 - LRK54_RS04195 0.19 +2.7 888,985 - LRK54_RS04195 0.19 +1.3 888,985 - LRK54_RS04195 0.19 +1.0 889,351 + +0.4 889,351 + +1.0 889,351 + -0.2 889,352 - -0.2 889,352 - +0.3 889,352 - +0.9 889,352 - +0.9 889,352 - +0.3 889,386 + +3.9 889,386 + -0.7 889,386 + -0.1 889,386 + +4.4 889,386 + -0.3 889,386 + -1.2 889,386 + +1.0 889,386 + -0.5 889,386 + +1.2 889,386 + +1.5 889,386 + +1.5 889,386 + +0.0 889,386 + -0.1 889,386 + -1.0 889,386 + +0.0 889,386 + +2.5 889,386 + -2.9 889,386 + -1.5 889,386 + +1.3 889,386 + -2.9 889,386 + +1.1 889,386 + +1.0 889,386 + +2.9 889,386 + +2.1 889,386 + +1.4 889,387 - -2.6 889,387 - +0.2 889,387 - -1.1 889,387 - -2.1 889,387 - +1.0 889,387 - -1.3 889,387 - -0.5 889,387 - -0.6 889,387 - -0.9 889,387 - -0.5 889,387 - +1.9 889,387 - -3.0 889,387 - -0.7 889,387 - -1.2 889,387 - -2.9 889,387 - +0.8 889,387 - -0.7 889,387 - +0.3 889,387 - -1.8 889,387 - +0.7 889,387 - +0.0 889,387 - -0.3 889,387 - -0.3 889,387 - -2.4 889,387 - -1.0 889,387 - +0.9 889,387 - +1.4 889,387 - +1.6 889,387 - -1.9 889,387 - +0.9 889,387 - +1.9 889,387 - -0.6 889,387 - -0.3 889,387 - +0.9 889,387 - +1.0 889,387 - -1.3 889,387 - -2.5 889,394 + +1.7 889,394 + +1.3 889,394 + -0.5 889,395 - +0.5 889,395 - +0.9 889,429 + +2.7 889,429 + +0.6 889,429 + +2.3 889,429 + -2.1 889,429 + +1.6 889,429 + -0.1 889,429 + +1.9 889,429 + +0.6 889,429 + -1.3 889,430 - +0.3 889,430 - +0.5 889,430 - +1.4 889,430 - -0.7 889,430 - +4.1 889,430 - +0.3 889,430 - -2.5 889,430 - -1.4 889,430 - -1.0 889,459 + +0.6 889,459 + +2.3 889,460 - -1.8 889,460 - -0.5 889,489 + +1.2 889,489 + -1.4 889,490 - +1.3 889,490 - -1.2 889,490 - -0.1 889,490 - +0.4
Or see this region's nucleotide sequence