Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00220 and MPMX20_00221 are separated by 9 nucleotides MPMX20_00221 and MPMX20_00222 are separated by 44 nucleotides MPMX20_00222 and MPMX20_00223 are separated by 186 nucleotides MPMX20_00223 and MPMX20_00224 are separated by 11 nucleotides
MPMX20_00220: MPMX20_00220 - Uroporphyrinogen decarboxylase, at 229,258 to 230,322
_00220
MPMX20_00221: MPMX20_00221 - Endonuclease V, at 230,332 to 231,000
_00221
MPMX20_00222: MPMX20_00222 - hypothetical protein, at 231,045 to 231,635
_00222
MPMX20_00223: MPMX20_00223 - DNA-binding protein HU-alpha, at 231,822 to 232,094
_00223
MPMX20_00224: MPMX20_00224 - hypothetical protein, at 232,106 to 232,798
_00224
Position (kb) 231
232 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 230.324 kb on - strand at 230.327 kb on + strand at 230.359 kb on - strand at 230.377 kb on + strand at 230.395 kb on + strand at 230.403 kb on - strand, within MPMX20_00221 at 230.442 kb on - strand, within MPMX20_00221 at 230.442 kb on - strand, within MPMX20_00221 at 230.498 kb on + strand, within MPMX20_00221 at 230.506 kb on - strand, within MPMX20_00221 at 230.514 kb on + strand, within MPMX20_00221 at 230.514 kb on + strand, within MPMX20_00221 at 230.514 kb on + strand, within MPMX20_00221 at 230.522 kb on - strand, within MPMX20_00221 at 230.552 kb on + strand, within MPMX20_00221 at 230.650 kb on - strand, within MPMX20_00221 at 230.656 kb on + strand, within MPMX20_00221 at 230.656 kb on + strand, within MPMX20_00221 at 230.674 kb on - strand, within MPMX20_00221 at 230.762 kb on - strand, within MPMX20_00221 at 230.798 kb on - strand, within MPMX20_00221 at 230.882 kb on + strand, within MPMX20_00221 at 230.884 kb on + strand, within MPMX20_00221 at 230.896 kb on + strand, within MPMX20_00221 at 230.896 kb on + strand, within MPMX20_00221 at 230.922 kb on - strand, within MPMX20_00221 at 230.956 kb on + strand at 231.016 kb on - strand at 231.179 kb on - strand, within MPMX20_00222 at 231.305 kb on + strand, within MPMX20_00222 at 231.363 kb on + strand, within MPMX20_00222 at 231.532 kb on + strand, within MPMX20_00222 at 231.532 kb on + strand, within MPMX20_00222 at 231.564 kb on - strand, within MPMX20_00222 at 231.608 kb on - strand at 231.707 kb on + strand at 231.805 kb on - strand at 231.837 kb on - strand at 231.876 kb on + strand, within MPMX20_00223 at 231.877 kb on - strand, within MPMX20_00223 at 231.881 kb on + strand, within MPMX20_00223 at 231.881 kb on + strand, within MPMX20_00223 at 231.889 kb on - strand, within MPMX20_00223 at 231.889 kb on - strand, within MPMX20_00223 at 231.889 kb on - strand, within MPMX20_00223 at 231.889 kb on - strand, within MPMX20_00223 at 231.889 kb on - strand, within MPMX20_00223 at 231.943 kb on + strand, within MPMX20_00223 at 231.961 kb on - strand, within MPMX20_00223 at 231.961 kb on - strand, within MPMX20_00223 at 232.183 kb on - strand, within MPMX20_00224 at 232.246 kb on + strand, within MPMX20_00224 at 232.300 kb on + strand, within MPMX20_00224 at 232.329 kb on + strand, within MPMX20_00224 at 232.404 kb on + strand, within MPMX20_00224 at 232.449 kb on + strand, within MPMX20_00224 at 232.457 kb on - strand, within MPMX20_00224 at 232.457 kb on - strand, within MPMX20_00224 at 232.481 kb on - strand, within MPMX20_00224 at 232.540 kb on + strand, within MPMX20_00224 at 232.548 kb on - strand, within MPMX20_00224 at 232.566 kb on + strand, within MPMX20_00224 at 232.574 kb on - strand, within MPMX20_00224 at 232.607 kb on + strand, within MPMX20_00224 at 232.615 kb on - strand, within MPMX20_00224 at 232.622 kb on + strand, within MPMX20_00224
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 230,324 - +0.1 230,327 + +2.4 230,359 - -0.4 230,377 + -0.4 230,395 + -0.0 230,403 - MPMX20_00221 0.11 +0.4 230,442 - MPMX20_00221 0.16 -1.8 230,442 - MPMX20_00221 0.16 -0.4 230,498 + MPMX20_00221 0.25 +1.3 230,506 - MPMX20_00221 0.26 +0.5 230,514 + MPMX20_00221 0.27 +0.8 230,514 + MPMX20_00221 0.27 -1.8 230,514 + MPMX20_00221 0.27 -2.1 230,522 - MPMX20_00221 0.28 -0.2 230,552 + MPMX20_00221 0.33 +3.7 230,650 - MPMX20_00221 0.48 -0.2 230,656 + MPMX20_00221 0.48 -0.2 230,656 + MPMX20_00221 0.48 +0.2 230,674 - MPMX20_00221 0.51 -1.1 230,762 - MPMX20_00221 0.64 +1.1 230,798 - MPMX20_00221 0.70 +0.0 230,882 + MPMX20_00221 0.82 -1.1 230,884 + MPMX20_00221 0.83 +0.0 230,896 + MPMX20_00221 0.84 -0.1 230,896 + MPMX20_00221 0.84 -0.2 230,922 - MPMX20_00221 0.88 -0.2 230,956 + +0.9 231,016 - +0.1 231,179 - MPMX20_00222 0.23 -0.9 231,305 + MPMX20_00222 0.44 -0.9 231,363 + MPMX20_00222 0.54 -1.1 231,532 + MPMX20_00222 0.82 -1.4 231,532 + MPMX20_00222 0.82 +0.3 231,564 - MPMX20_00222 0.88 -0.7 231,608 - +0.3 231,707 + +0.0 231,805 - +0.0 231,837 - -0.4 231,876 + MPMX20_00223 0.20 +0.3 231,877 - MPMX20_00223 0.20 +1.9 231,881 + MPMX20_00223 0.22 +0.5 231,881 + MPMX20_00223 0.22 +0.2 231,889 - MPMX20_00223 0.25 +1.8 231,889 - MPMX20_00223 0.25 +0.5 231,889 - MPMX20_00223 0.25 +0.4 231,889 - MPMX20_00223 0.25 +0.1 231,889 - MPMX20_00223 0.25 -0.6 231,943 + MPMX20_00223 0.44 -0.4 231,961 - MPMX20_00223 0.51 -0.3 231,961 - MPMX20_00223 0.51 -0.3 232,183 - MPMX20_00224 0.11 +0.7 232,246 + MPMX20_00224 0.20 +0.4 232,300 + MPMX20_00224 0.28 +0.5 232,329 + MPMX20_00224 0.32 +0.1 232,404 + MPMX20_00224 0.43 -0.0 232,449 + MPMX20_00224 0.49 -0.2 232,457 - MPMX20_00224 0.51 -1.8 232,457 - MPMX20_00224 0.51 +0.2 232,481 - MPMX20_00224 0.54 -2.2 232,540 + MPMX20_00224 0.63 +0.5 232,548 - MPMX20_00224 0.64 -0.5 232,566 + MPMX20_00224 0.66 +2.1 232,574 - MPMX20_00224 0.68 +0.2 232,607 + MPMX20_00224 0.72 -0.3 232,615 - MPMX20_00224 0.73 +0.4 232,622 + MPMX20_00224 0.74 +0.4
Or see this region's nucleotide sequence