Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02474 and MPMX20_02475 overlap by 4 nucleotides MPMX20_02475 and MPMX20_02476 overlap by 14 nucleotides MPMX20_02476 and MPMX20_02477 overlap by 1 nucleotides MPMX20_02477 and MPMX20_02478 are separated by 1 nucleotides
MPMX20_02474: MPMX20_02474 - Peptide transport periplasmic protein SapA, at 2,613,982 to 2,615,622
_02474
MPMX20_02475: MPMX20_02475 - Putrescine export system permease protein SapB, at 2,615,619 to 2,616,584
_02475
MPMX20_02476: MPMX20_02476 - Putrescine export system permease protein SapC, at 2,616,571 to 2,617,461
_02476
MPMX20_02477: MPMX20_02477 - Peptide transport system ATP-binding protein SapD, at 2,617,461 to 2,618,453
_02477
MPMX20_02478: MPMX20_02478 - Putrescine export system ATP-binding protein SapF, at 2,618,455 to 2,619,261
_02478
Position (kb)
2616
2617
2618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2615.576 kb on + strand at 2615.685 kb on - strand at 2615.709 kb on - strand at 2615.823 kb on - strand, within MPMX20_02475 at 2615.823 kb on - strand, within MPMX20_02475 at 2615.823 kb on - strand, within MPMX20_02475 at 2615.825 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.856 kb on - strand, within MPMX20_02475 at 2615.960 kb on + strand, within MPMX20_02475 at 2615.964 kb on - strand, within MPMX20_02475 at 2616.022 kb on + strand, within MPMX20_02475 at 2616.027 kb on + strand, within MPMX20_02475 at 2616.126 kb on + strand, within MPMX20_02475 at 2616.172 kb on + strand, within MPMX20_02475 at 2616.235 kb on + strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.328 kb on - strand, within MPMX20_02475 at 2616.412 kb on + strand, within MPMX20_02475 at 2616.425 kb on - strand, within MPMX20_02475 at 2616.425 kb on - strand, within MPMX20_02475 at 2616.542 kb on + strand at 2616.585 kb on + strand at 2616.721 kb on - strand, within MPMX20_02476 at 2616.789 kb on + strand, within MPMX20_02476 at 2616.931 kb on - strand, within MPMX20_02476 at 2616.981 kb on - strand, within MPMX20_02476 at 2617.099 kb on + strand, within MPMX20_02476 at 2617.150 kb on + strand, within MPMX20_02476 at 2617.158 kb on - strand, within MPMX20_02476 at 2617.296 kb on + strand, within MPMX20_02476 at 2617.349 kb on + strand, within MPMX20_02476 at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.434 kb on - strand at 2617.434 kb on - strand at 2617.540 kb on + strand at 2617.546 kb on + strand at 2617.548 kb on - strand at 2617.601 kb on - strand, within MPMX20_02477 at 2617.651 kb on + strand, within MPMX20_02477 at 2617.731 kb on - strand, within MPMX20_02477 at 2617.762 kb on + strand, within MPMX20_02477 at 2617.762 kb on + strand, within MPMX20_02477 at 2618.050 kb on - strand, within MPMX20_02477 at 2618.076 kb on - strand, within MPMX20_02477 at 2618.115 kb on - strand, within MPMX20_02477 at 2618.188 kb on + strand, within MPMX20_02477 at 2618.195 kb on + strand, within MPMX20_02477 at 2618.201 kb on - strand, within MPMX20_02477 at 2618.203 kb on - strand, within MPMX20_02477 at 2618.203 kb on - strand, within MPMX20_02477 at 2618.260 kb on + strand, within MPMX20_02477 at 2618.299 kb on + strand, within MPMX20_02477 at 2618.312 kb on + strand, within MPMX20_02477 at 2618.312 kb on - strand, within MPMX20_02477 at 2618.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 2,615,576 + -1.5 2,615,685 - -1.1 2,615,709 - -2.4 2,615,823 - MPMX20_02475 0.21 -1.7 2,615,823 - MPMX20_02475 0.21 -1.8 2,615,823 - MPMX20_02475 0.21 -2.5 2,615,825 + MPMX20_02475 0.21 -0.8 2,615,848 + MPMX20_02475 0.24 -1.4 2,615,848 + MPMX20_02475 0.24 -1.4 2,615,848 + MPMX20_02475 0.24 -1.4 2,615,848 + MPMX20_02475 0.24 -0.8 2,615,856 - MPMX20_02475 0.25 -1.8 2,615,960 + MPMX20_02475 0.35 +0.6 2,615,964 - MPMX20_02475 0.36 -1.9 2,616,022 + MPMX20_02475 0.42 -2.9 2,616,027 + MPMX20_02475 0.42 -0.8 2,616,126 + MPMX20_02475 0.52 -1.4 2,616,172 + MPMX20_02475 0.57 -1.6 2,616,235 + MPMX20_02475 0.64 -0.7 2,616,243 - MPMX20_02475 0.65 -0.4 2,616,243 - MPMX20_02475 0.65 -0.4 2,616,243 - MPMX20_02475 0.65 -1.4 2,616,328 - MPMX20_02475 0.73 -1.4 2,616,412 + MPMX20_02475 0.82 -3.1 2,616,425 - MPMX20_02475 0.83 -2.1 2,616,425 - MPMX20_02475 0.83 -0.4 2,616,542 + -0.5 2,616,585 + -3.5 2,616,721 - MPMX20_02476 0.17 -0.8 2,616,789 + MPMX20_02476 0.24 +0.6 2,616,931 - MPMX20_02476 0.40 -1.6 2,616,981 - MPMX20_02476 0.46 -1.6 2,617,099 + MPMX20_02476 0.59 -1.8 2,617,150 + MPMX20_02476 0.65 -2.7 2,617,158 - MPMX20_02476 0.66 -0.8 2,617,296 + MPMX20_02476 0.81 +3.2 2,617,349 + MPMX20_02476 0.87 -1.4 2,617,426 + -1.7 2,617,426 + -0.5 2,617,426 + -1.1 2,617,426 + -3.2 2,617,434 - -3.2 2,617,434 - -0.5 2,617,540 + -1.6 2,617,546 + -1.8 2,617,548 - -1.1 2,617,601 - MPMX20_02477 0.14 -0.4 2,617,651 + MPMX20_02477 0.19 +1.2 2,617,731 - MPMX20_02477 0.27 -0.4 2,617,762 + MPMX20_02477 0.30 -1.1 2,617,762 + MPMX20_02477 0.30 -1.9 2,618,050 - MPMX20_02477 0.59 -2.9 2,618,076 - MPMX20_02477 0.62 -2.1 2,618,115 - MPMX20_02477 0.66 -0.4 2,618,188 + MPMX20_02477 0.73 -2.6 2,618,195 + MPMX20_02477 0.74 -0.8 2,618,201 - MPMX20_02477 0.75 -2.6 2,618,203 - MPMX20_02477 0.75 -0.9 2,618,203 - MPMX20_02477 0.75 +2.2 2,618,260 + MPMX20_02477 0.80 +0.2 2,618,299 + MPMX20_02477 0.84 -1.6 2,618,312 + MPMX20_02477 0.86 -1.6 2,618,312 - MPMX20_02477 0.86 -2.6 2,618,457 + -1.4
Or see this region's nucleotide sequence