Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01450 and MMJJ_RS01455 are separated by 164 nucleotides MMJJ_RS01455 and MMJJ_RS01460 are separated by 78 nucleotides MMJJ_RS01460 and MMJJ_RS01465 are separated by 399 nucleotides
MMJJ_RS01450: MMJJ_RS01450 - iron ABC transporter substrate-binding protein, at 283,642 to 284,769
_RS01450
MMJJ_RS01455: MMJJ_RS01455 - triose-phosphate isomerase, at 284,934 to 285,599
_RS01455
MMJJ_RS01460: MMJJ_RS01460 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, at 285,678 to 286,496
_RS01460
MMJJ_RS01465: MMJJ_RS01465 - METTL5 family protein, at 286,896 to 287,537
_RS01465
Position (kb) 285
286
287 Strain fitness (log2 ratio) -2
-1
0
1 at 284.697 kb on - strand at 284.698 kb on + strand at 284.709 kb on - strand at 284.769 kb on - strand at 284.770 kb on + strand at 284.796 kb on - strand at 284.824 kb on + strand at 284.837 kb on - strand at 284.842 kb on + strand at 284.878 kb on - strand at 284.929 kb on - strand at 285.481 kb on + strand, within MMJJ_RS01455 at 285.574 kb on - strand at 285.586 kb on + strand at 285.599 kb on - strand at 285.619 kb on + strand at 285.620 kb on - strand at 285.624 kb on + strand at 285.644 kb on - strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.649 kb on + strand at 285.696 kb on - strand at 286.105 kb on - strand, within MMJJ_RS01460 at 286.543 kb on - strand at 286.546 kb on + strand at 286.547 kb on - strand at 286.547 kb on - strand at 286.548 kb on + strand at 286.548 kb on + strand at 286.548 kb on + strand at 286.621 kb on + strand at 286.783 kb on - strand at 286.837 kb on - strand at 286.840 kb on + strand at 286.842 kb on + strand at 286.914 kb on + strand at 286.987 kb on + strand, within MMJJ_RS01465 at 286.987 kb on + strand, within MMJJ_RS01465 at 286.989 kb on + strand, within MMJJ_RS01465 at 286.989 kb on + strand at 287.020 kb on - strand, within MMJJ_RS01465 at 287.020 kb on - strand, within MMJJ_RS01465 at 287.021 kb on + strand, within MMJJ_RS01465 at 287.021 kb on + strand, within MMJJ_RS01465 at 287.021 kb on + strand, within MMJJ_RS01465 at 287.055 kb on - strand, within MMJJ_RS01465 at 287.060 kb on - strand, within MMJJ_RS01465 at 287.169 kb on + strand, within MMJJ_RS01465 at 287.214 kb on + strand, within MMJJ_RS01465 at 287.260 kb on + strand, within MMJJ_RS01465 at 287.260 kb on + strand, within MMJJ_RS01465 at 287.359 kb on + strand, within MMJJ_RS01465 at 287.360 kb on - strand, within MMJJ_RS01465 at 287.361 kb on + strand, within MMJJ_RS01465 at 287.382 kb on - strand, within MMJJ_RS01465 at 287.408 kb on - strand, within MMJJ_RS01465
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 284,697 - +0.4 284,698 + +1.0 284,709 - -1.0 284,769 - -0.5 284,770 + -0.7 284,796 - -0.4 284,824 + +0.3 284,837 - -0.9 284,842 + +0.3 284,878 - -0.4 284,929 - +0.0 285,481 + MMJJ_RS01455 0.82 -1.6 285,574 - -1.8 285,586 + -2.4 285,599 - -0.4 285,619 + +0.3 285,620 - -2.2 285,624 + -1.5 285,644 - -1.1 285,649 + -0.9 285,649 + -0.9 285,649 + -0.8 285,696 - -1.1 286,105 - MMJJ_RS01460 0.52 -1.4 286,543 - -0.7 286,546 + +0.3 286,547 - -1.6 286,547 - -1.7 286,548 + +0.1 286,548 + -2.0 286,548 + -1.1 286,621 + +0.8 286,783 - +0.3 286,837 - -0.2 286,840 + +1.5 286,842 + -1.8 286,914 + -0.4 286,987 + MMJJ_RS01465 0.14 -1.6 286,987 + MMJJ_RS01465 0.14 +0.6 286,989 + MMJJ_RS01465 0.14 +0.8 286,989 + +0.6 287,020 - MMJJ_RS01465 0.19 +1.2 287,020 - MMJJ_RS01465 0.19 +0.6 287,021 + MMJJ_RS01465 0.19 +1.4 287,021 + MMJJ_RS01465 0.19 +0.3 287,021 + MMJJ_RS01465 0.19 +1.2 287,055 - MMJJ_RS01465 0.25 +0.7 287,060 - MMJJ_RS01465 0.26 +0.8 287,169 + MMJJ_RS01465 0.43 +0.4 287,214 + MMJJ_RS01465 0.50 +1.3 287,260 + MMJJ_RS01465 0.57 +0.4 287,260 + MMJJ_RS01465 0.57 -1.6 287,359 + MMJJ_RS01465 0.72 -0.3 287,360 - MMJJ_RS01465 0.72 +0.2 287,361 + MMJJ_RS01465 0.72 +0.9 287,382 - MMJJ_RS01465 0.76 +0.8 287,408 - MMJJ_RS01465 0.80 +0.5
Or see this region's nucleotide sequence