Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08510 and MMJJ_RS08515 are separated by 73 nucleotides MMJJ_RS08515 and MMJJ_RS08520 are separated by 114 nucleotides MMJJ_RS08520 and MMJJ_RS08525 are separated by 41 nucleotides
MMJJ_RS08510: MMJJ_RS08510 - peptide chain release factor aRF-1, at 1,565,687 to 1,566,946
_RS08510
MMJJ_RS08515: MMJJ_RS08515 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,567,020 to 1,567,418
_RS08515
MMJJ_RS08520: MMJJ_RS08520 - peptidylprolyl isomerase, at 1,567,533 to 1,568,021
_RS08520
MMJJ_RS08525: MMJJ_RS08525 - peptidylprolyl isomerase, at 1,568,063 to 1,568,665
_RS08525
Position (kb)
1567
1568 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8
9
10 at 1566.838 kb on - strand at 1566.944 kb on - strand at 1566.944 kb on - strand at 1566.964 kb on + strand at 1566.980 kb on - strand at 1566.981 kb on + strand at 1566.987 kb on + strand at 1566.991 kb on + strand at 1567.001 kb on + strand at 1567.020 kb on + strand at 1567.088 kb on + strand, within MMJJ_RS08515 at 1567.148 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.166 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.315 kb on - strand, within MMJJ_RS08515 at 1567.316 kb on + strand, within MMJJ_RS08515 at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.422 kb on + strand at 1567.442 kb on + strand at 1567.449 kb on + strand at 1567.454 kb on + strand at 1567.573 kb on - strand at 1567.579 kb on - strand at 1567.579 kb on - strand at 1567.666 kb on - strand, within MMJJ_RS08520 at 1567.808 kb on + strand, within MMJJ_RS08520 at 1567.835 kb on + strand, within MMJJ_RS08520 at 1567.966 kb on + strand, within MMJJ_RS08520 at 1568.010 kb on + strand at 1568.037 kb on - strand at 1568.038 kb on + strand at 1568.089 kb on + strand at 1568.132 kb on + strand, within MMJJ_RS08525 at 1568.172 kb on - strand, within MMJJ_RS08525 at 1568.173 kb on + strand, within MMJJ_RS08525 at 1568.178 kb on + strand, within MMJJ_RS08525 at 1568.310 kb on - strand at 1568.311 kb on + strand, within MMJJ_RS08525
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 1,566,838 - -0.8 1,566,944 - -0.1 1,566,944 - +0.6 1,566,964 + +0.7 1,566,980 - +0.8 1,566,981 + +0.4 1,566,987 + -1.5 1,566,991 + +0.5 1,567,001 + -0.1 1,567,020 + -0.0 1,567,088 + MMJJ_RS08515 0.17 -0.2 1,567,148 - MMJJ_RS08515 0.32 +0.4 1,567,165 - MMJJ_RS08515 0.36 +0.8 1,567,165 - MMJJ_RS08515 0.36 +1.0 1,567,166 + MMJJ_RS08515 0.37 +0.5 1,567,252 + MMJJ_RS08515 0.58 +0.5 1,567,252 + MMJJ_RS08515 0.58 +9.8 1,567,315 - MMJJ_RS08515 0.74 +1.0 1,567,316 + MMJJ_RS08515 0.74 -0.5 1,567,421 - +0.5 1,567,421 - -0.1 1,567,421 - -0.4 1,567,422 + +0.3 1,567,442 + +0.7 1,567,449 + +0.1 1,567,454 + -0.0 1,567,573 - -0.0 1,567,579 - -0.5 1,567,579 - -0.7 1,567,666 - MMJJ_RS08520 0.27 -0.3 1,567,808 + MMJJ_RS08520 0.56 -0.6 1,567,835 + MMJJ_RS08520 0.62 -1.3 1,567,966 + MMJJ_RS08520 0.89 -0.2 1,568,010 + +0.7 1,568,037 - +0.4 1,568,038 + +0.4 1,568,089 + -0.0 1,568,132 + MMJJ_RS08525 0.11 +0.5 1,568,172 - MMJJ_RS08525 0.18 -0.2 1,568,173 + MMJJ_RS08525 0.18 -0.3 1,568,178 + MMJJ_RS08525 0.19 +0.4 1,568,310 - -0.4 1,568,311 + MMJJ_RS08525 0.41 +0.2
Or see this region's nucleotide sequence