Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06575 and MMJJ_RS06580 are separated by 295 nucleotides MMJJ_RS06580 and MMJJ_RS06585 are separated by 55 nucleotides
MMJJ_RS06575: MMJJ_RS06575 - prephenate dehydrogenase, at 1,213,317 to 1,214,651
_RS06575
MMJJ_RS06580: MMJJ_RS06580 - alanine dehydrogenase, at 1,214,947 to 1,216,068
_RS06580
MMJJ_RS06585: MMJJ_RS06585 - alanine racemase, at 1,216,124 to 1,217,245
_RS06585
Position (kb)
1214
1215
1216
1217 Strain fitness (log2 ratio)
-1
0
1
2 at 1214.096 kb on + strand, within MMJJ_RS06575 at 1214.122 kb on + strand, within MMJJ_RS06575 at 1214.122 kb on + strand, within MMJJ_RS06575 at 1214.298 kb on - strand at 1214.532 kb on - strand at 1214.668 kb on - strand at 1214.682 kb on - strand at 1214.685 kb on - strand at 1214.696 kb on - strand at 1214.696 kb on - strand at 1214.696 kb on - strand at 1214.696 kb on - strand at 1214.696 kb on - strand at 1214.696 kb on - strand at 1214.697 kb on + strand at 1214.697 kb on + strand at 1214.697 kb on + strand at 1214.697 kb on + strand at 1214.697 kb on + strand at 1214.700 kb on - strand at 1214.701 kb on + strand at 1214.701 kb on + strand at 1214.703 kb on - strand at 1214.723 kb on + strand at 1214.746 kb on - strand at 1214.746 kb on - strand at 1214.746 kb on - strand at 1214.751 kb on - strand at 1214.757 kb on - strand at 1214.758 kb on + strand at 1214.758 kb on + strand at 1214.758 kb on + strand at 1214.760 kb on - strand at 1214.816 kb on - strand at 1214.817 kb on + strand at 1214.820 kb on + strand at 1214.820 kb on + strand at 1214.820 kb on + strand at 1214.827 kb on + strand at 1214.827 kb on + strand at 1214.837 kb on - strand at 1214.841 kb on - strand at 1214.841 kb on - strand at 1214.872 kb on + strand at 1214.875 kb on - strand at 1214.875 kb on - strand at 1214.876 kb on + strand at 1214.876 kb on + strand at 1214.876 kb on + strand at 1214.891 kb on - strand at 1214.892 kb on + strand at 1214.892 kb on + strand at 1214.902 kb on + strand at 1214.906 kb on - strand at 1214.907 kb on + strand at 1214.951 kb on - strand at 1214.997 kb on - strand at 1215.014 kb on - strand at 1215.015 kb on + strand at 1215.044 kb on - strand at 1215.044 kb on - strand at 1215.046 kb on - strand at 1215.046 kb on - strand at 1215.047 kb on + strand at 1215.047 kb on + strand at 1215.094 kb on - strand, within MMJJ_RS06580 at 1215.095 kb on + strand, within MMJJ_RS06580 at 1215.169 kb on + strand, within MMJJ_RS06580 at 1215.226 kb on - strand, within MMJJ_RS06580 at 1215.226 kb on - strand, within MMJJ_RS06580 at 1215.226 kb on - strand, within MMJJ_RS06580 at 1215.226 kb on - strand, within MMJJ_RS06580 at 1215.226 kb on - strand, within MMJJ_RS06580 at 1215.227 kb on + strand, within MMJJ_RS06580 at 1215.227 kb on + strand, within MMJJ_RS06580 at 1215.255 kb on - strand, within MMJJ_RS06580 at 1215.255 kb on - strand, within MMJJ_RS06580 at 1215.255 kb on - strand, within MMJJ_RS06580 at 1215.255 kb on - strand, within MMJJ_RS06580 at 1215.256 kb on + strand, within MMJJ_RS06580 at 1215.326 kb on - strand, within MMJJ_RS06580 at 1215.326 kb on - strand, within MMJJ_RS06580 at 1215.327 kb on + strand, within MMJJ_RS06580 at 1215.327 kb on + strand, within MMJJ_RS06580 at 1215.335 kb on - strand, within MMJJ_RS06580 at 1215.336 kb on + strand, within MMJJ_RS06580 at 1215.388 kb on - strand, within MMJJ_RS06580 at 1215.389 kb on + strand, within MMJJ_RS06580 at 1215.422 kb on - strand, within MMJJ_RS06580 at 1215.468 kb on + strand, within MMJJ_RS06580 at 1215.468 kb on + strand, within MMJJ_RS06580 at 1215.487 kb on + strand, within MMJJ_RS06580 at 1215.510 kb on + strand, within MMJJ_RS06580 at 1215.537 kb on + strand, within MMJJ_RS06580 at 1215.563 kb on + strand, within MMJJ_RS06580 at 1215.563 kb on + strand, within MMJJ_RS06580 at 1215.573 kb on - strand, within MMJJ_RS06580 at 1215.574 kb on + strand, within MMJJ_RS06580 at 1215.599 kb on - strand, within MMJJ_RS06580 at 1215.599 kb on - strand, within MMJJ_RS06580 at 1215.600 kb on + strand, within MMJJ_RS06580 at 1215.666 kb on + strand, within MMJJ_RS06580 at 1215.811 kb on - strand, within MMJJ_RS06580 at 1215.877 kb on + strand, within MMJJ_RS06580 at 1215.877 kb on + strand, within MMJJ_RS06580 at 1215.877 kb on + strand, within MMJJ_RS06580 at 1215.880 kb on - strand, within MMJJ_RS06580 at 1215.881 kb on + strand, within MMJJ_RS06580 at 1215.915 kb on + strand, within MMJJ_RS06580 at 1215.947 kb on - strand, within MMJJ_RS06580 at 1215.977 kb on - strand at 1216.004 kb on + strand at 1216.004 kb on + strand at 1216.037 kb on + strand at 1216.067 kb on + strand at 1216.071 kb on + strand at 1216.082 kb on + strand at 1216.109 kb on - strand at 1216.109 kb on - strand at 1216.109 kb on - strand at 1216.112 kb on + strand at 1216.112 kb on + strand at 1216.112 kb on + strand at 1216.168 kb on - strand at 1216.169 kb on + strand at 1216.230 kb on - strand at 1216.350 kb on - strand, within MMJJ_RS06585 at 1216.368 kb on - strand, within MMJJ_RS06585 at 1216.419 kb on - strand, within MMJJ_RS06585 at 1216.419 kb on - strand, within MMJJ_RS06585 at 1216.514 kb on + strand, within MMJJ_RS06585 at 1216.642 kb on - strand, within MMJJ_RS06585 at 1216.649 kb on - strand, within MMJJ_RS06585 at 1216.649 kb on - strand, within MMJJ_RS06585 at 1216.650 kb on + strand, within MMJJ_RS06585 at 1216.650 kb on + strand, within MMJJ_RS06585 at 1216.658 kb on + strand at 1216.669 kb on - strand, within MMJJ_RS06585 at 1216.739 kb on + strand, within MMJJ_RS06585 at 1216.749 kb on - strand, within MMJJ_RS06585 at 1216.777 kb on - strand, within MMJJ_RS06585 at 1216.796 kb on + strand, within MMJJ_RS06585 at 1216.843 kb on - strand, within MMJJ_RS06585 at 1216.872 kb on - strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.878 kb on - strand, within MMJJ_RS06585 at 1216.878 kb on - strand, within MMJJ_RS06585 at 1216.879 kb on + strand, within MMJJ_RS06585 at 1216.887 kb on - strand, within MMJJ_RS06585 at 1216.887 kb on - strand, within MMJJ_RS06585 at 1216.888 kb on + strand, within MMJJ_RS06585 at 1216.888 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.976 kb on - strand, within MMJJ_RS06585
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 1,214,096 + MMJJ_RS06575 0.58 -1.1 1,214,122 + MMJJ_RS06575 0.60 -1.1 1,214,122 + MMJJ_RS06575 0.60 -0.8 1,214,298 - +0.0 1,214,532 - +0.7 1,214,668 - +0.3 1,214,682 - +0.5 1,214,685 - -0.5 1,214,696 - -1.0 1,214,696 - -0.3 1,214,696 - -0.2 1,214,696 - -0.9 1,214,696 - +0.3 1,214,696 - -0.2 1,214,697 + -1.1 1,214,697 + +0.4 1,214,697 + +0.2 1,214,697 + +0.1 1,214,697 + -0.5 1,214,700 - +0.1 1,214,701 + -0.8 1,214,701 + +0.3 1,214,703 - +1.7 1,214,723 + -0.9 1,214,746 - +0.4 1,214,746 - +2.3 1,214,746 - +0.9 1,214,751 - +0.0 1,214,757 - +0.4 1,214,758 + +0.3 1,214,758 + +0.3 1,214,758 + +0.3 1,214,760 - -0.1 1,214,816 - +0.0 1,214,817 + +0.2 1,214,820 + +0.2 1,214,820 + -0.6 1,214,820 + -0.4 1,214,827 + +0.6 1,214,827 + +0.5 1,214,837 - +0.2 1,214,841 - -0.1 1,214,841 - +0.3 1,214,872 + +0.2 1,214,875 - +0.1 1,214,875 - +0.1 1,214,876 + +0.4 1,214,876 + +0.2 1,214,876 + +1.1 1,214,891 - -0.6 1,214,892 + +0.4 1,214,892 + +0.2 1,214,902 + -0.5 1,214,906 - -0.2 1,214,907 + +0.2 1,214,951 - +0.6 1,214,997 - +0.5 1,215,014 - -0.1 1,215,015 + +0.2 1,215,044 - +0.1 1,215,044 - -0.1 1,215,046 - +0.5 1,215,046 - +1.2 1,215,047 + -0.0 1,215,047 + -0.4 1,215,094 - MMJJ_RS06580 0.13 +0.0 1,215,095 + MMJJ_RS06580 0.13 +0.6 1,215,169 + MMJJ_RS06580 0.20 +0.0 1,215,226 - MMJJ_RS06580 0.25 +0.2 1,215,226 - MMJJ_RS06580 0.25 -0.3 1,215,226 - MMJJ_RS06580 0.25 +0.5 1,215,226 - MMJJ_RS06580 0.25 -0.2 1,215,226 - MMJJ_RS06580 0.25 -0.2 1,215,227 + MMJJ_RS06580 0.25 +0.6 1,215,227 + MMJJ_RS06580 0.25 +0.2 1,215,255 - MMJJ_RS06580 0.27 +0.6 1,215,255 - MMJJ_RS06580 0.27 +0.5 1,215,255 - MMJJ_RS06580 0.27 +0.1 1,215,255 - MMJJ_RS06580 0.27 -0.1 1,215,256 + MMJJ_RS06580 0.28 +0.4 1,215,326 - MMJJ_RS06580 0.34 +0.5 1,215,326 - MMJJ_RS06580 0.34 +0.4 1,215,327 + MMJJ_RS06580 0.34 -0.1 1,215,327 + MMJJ_RS06580 0.34 +0.4 1,215,335 - MMJJ_RS06580 0.35 +0.0 1,215,336 + MMJJ_RS06580 0.35 +1.3 1,215,388 - MMJJ_RS06580 0.39 -0.1 1,215,389 + MMJJ_RS06580 0.39 +0.1 1,215,422 - MMJJ_RS06580 0.42 +0.7 1,215,468 + MMJJ_RS06580 0.46 -0.3 1,215,468 + MMJJ_RS06580 0.46 +0.1 1,215,487 + MMJJ_RS06580 0.48 -0.4 1,215,510 + MMJJ_RS06580 0.50 -0.2 1,215,537 + MMJJ_RS06580 0.53 +1.0 1,215,563 + MMJJ_RS06580 0.55 +0.7 1,215,563 + MMJJ_RS06580 0.55 +0.8 1,215,573 - MMJJ_RS06580 0.56 -0.3 1,215,574 + MMJJ_RS06580 0.56 +0.3 1,215,599 - MMJJ_RS06580 0.58 +0.2 1,215,599 - MMJJ_RS06580 0.58 +0.4 1,215,600 + MMJJ_RS06580 0.58 -0.1 1,215,666 + MMJJ_RS06580 0.64 -0.7 1,215,811 - MMJJ_RS06580 0.77 +1.6 1,215,877 + MMJJ_RS06580 0.83 +0.1 1,215,877 + MMJJ_RS06580 0.83 +1.0 1,215,877 + MMJJ_RS06580 0.83 +0.3 1,215,880 - MMJJ_RS06580 0.83 +0.2 1,215,881 + MMJJ_RS06580 0.83 -0.1 1,215,915 + MMJJ_RS06580 0.86 -0.4 1,215,947 - MMJJ_RS06580 0.89 -0.1 1,215,977 - -0.3 1,216,004 + +0.6 1,216,004 + +0.3 1,216,037 + +1.4 1,216,067 + -0.1 1,216,071 + -0.1 1,216,082 + +0.4 1,216,109 - +0.2 1,216,109 - +0.8 1,216,109 - +0.2 1,216,112 + +1.6 1,216,112 + +0.3 1,216,112 + +0.1 1,216,168 - +0.6 1,216,169 + +0.5 1,216,230 - +0.0 1,216,350 - MMJJ_RS06585 0.20 -0.0 1,216,368 - MMJJ_RS06585 0.22 +0.5 1,216,419 - MMJJ_RS06585 0.26 +0.8 1,216,419 - MMJJ_RS06585 0.26 +0.8 1,216,514 + MMJJ_RS06585 0.35 +0.4 1,216,642 - MMJJ_RS06585 0.46 +1.0 1,216,649 - MMJJ_RS06585 0.47 -0.0 1,216,649 - MMJJ_RS06585 0.47 -0.0 1,216,650 + MMJJ_RS06585 0.47 +0.5 1,216,650 + MMJJ_RS06585 0.47 +1.2 1,216,658 + +0.5 1,216,669 - MMJJ_RS06585 0.49 +0.5 1,216,739 + MMJJ_RS06585 0.55 +1.0 1,216,749 - MMJJ_RS06585 0.56 -0.4 1,216,777 - MMJJ_RS06585 0.58 +0.0 1,216,796 + MMJJ_RS06585 0.60 -0.0 1,216,843 - MMJJ_RS06585 0.64 -0.3 1,216,872 - MMJJ_RS06585 0.67 +0.8 1,216,873 + MMJJ_RS06585 0.67 -0.2 1,216,873 + MMJJ_RS06585 0.67 +0.4 1,216,873 + MMJJ_RS06585 0.67 +0.7 1,216,878 - MMJJ_RS06585 0.67 +0.1 1,216,878 - MMJJ_RS06585 0.67 +0.0 1,216,879 + MMJJ_RS06585 0.67 -0.1 1,216,887 - MMJJ_RS06585 0.68 +0.4 1,216,887 - MMJJ_RS06585 0.68 +0.6 1,216,888 + MMJJ_RS06585 0.68 +0.1 1,216,888 + MMJJ_RS06585 0.68 +1.4 1,216,920 + MMJJ_RS06585 0.71 +0.2 1,216,920 + MMJJ_RS06585 0.71 +1.9 1,216,920 + MMJJ_RS06585 0.71 +0.4 1,216,976 - MMJJ_RS06585 0.76 +0.8
Or see this region's nucleotide sequence