Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05145 and MMJJ_RS05150 are separated by 229 nucleotides MMJJ_RS05150 and MMJJ_RS05155 are separated by 115 nucleotides
MMJJ_RS05145: MMJJ_RS05145 - 5,10-methylenetetrahydromethanopterin reductase, at 952,092 to 953,054
_RS05145
MMJJ_RS05150: MMJJ_RS05150 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 953,284 to 954,360
_RS05150
MMJJ_RS05155: MMJJ_RS05155 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 954,476 to 955,555
_RS05155
Position (kb) 953
954
955 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 952.390 kb on + strand at 952.747 kb on + strand at 953.096 kb on - strand at 953.104 kb on + strand at 953.152 kb on - strand at 953.152 kb on - strand at 953.237 kb on + strand at 953.237 kb on + strand at 953.241 kb on + strand at 953.340 kb on + strand at 953.350 kb on + strand at 953.391 kb on - strand at 953.392 kb on + strand, within MMJJ_RS05150 at 953.460 kb on - strand, within MMJJ_RS05150 at 953.473 kb on + strand, within MMJJ_RS05150 at 953.553 kb on - strand, within MMJJ_RS05150 at 953.575 kb on + strand, within MMJJ_RS05150 at 953.584 kb on + strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.644 kb on + strand, within MMJJ_RS05150 at 953.679 kb on + strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.692 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.737 kb on + strand, within MMJJ_RS05150 at 953.822 kb on - strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.871 kb on - strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.901 kb on - strand, within MMJJ_RS05150 at 953.902 kb on + strand, within MMJJ_RS05150 at 953.950 kb on + strand, within MMJJ_RS05150 at 954.029 kb on - strand, within MMJJ_RS05150 at 954.030 kb on + strand, within MMJJ_RS05150 at 954.066 kb on - strand, within MMJJ_RS05150 at 954.088 kb on - strand, within MMJJ_RS05150 at 954.127 kb on + strand, within MMJJ_RS05150 at 954.135 kb on - strand, within MMJJ_RS05150 at 954.238 kb on + strand, within MMJJ_RS05150 at 954.261 kb on - strand at 954.262 kb on + strand at 954.262 kb on + strand at 954.265 kb on - strand at 954.265 kb on - strand at 954.266 kb on + strand at 954.266 kb on + strand at 954.281 kb on - strand at 954.287 kb on - strand at 954.288 kb on + strand at 954.303 kb on - strand at 954.304 kb on + strand at 954.304 kb on + strand at 954.350 kb on - strand at 954.351 kb on + strand at 954.359 kb on + strand at 954.389 kb on - strand at 954.390 kb on + strand at 954.400 kb on - strand at 954.401 kb on + strand at 954.401 kb on + strand at 954.451 kb on - strand at 954.452 kb on + strand at 954.456 kb on + strand at 954.614 kb on - strand, within MMJJ_RS05155 at 954.614 kb on - strand, within MMJJ_RS05155 at 954.614 kb on - strand, within MMJJ_RS05155 at 954.615 kb on + strand, within MMJJ_RS05155 at 954.632 kb on + strand at 954.812 kb on - strand, within MMJJ_RS05155 at 954.927 kb on - strand, within MMJJ_RS05155 at 955.014 kb on - strand, within MMJJ_RS05155 at 955.015 kb on + strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155 at 955.325 kb on - strand, within MMJJ_RS05155
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 952,390 + -0.2 952,747 + -0.5 953,096 - -0.8 953,104 + -1.3 953,152 - -0.6 953,152 - +0.5 953,237 + -1.1 953,237 + -0.7 953,241 + -2.1 953,340 + -1.5 953,350 + -3.1 953,391 - -1.3 953,392 + MMJJ_RS05150 0.10 -1.7 953,460 - MMJJ_RS05150 0.16 -1.5 953,473 + MMJJ_RS05150 0.18 -2.8 953,553 - MMJJ_RS05150 0.25 -0.9 953,575 + MMJJ_RS05150 0.27 -2.3 953,584 + MMJJ_RS05150 0.28 -2.1 953,643 - MMJJ_RS05150 0.33 -2.3 953,643 - MMJJ_RS05150 0.33 -2.3 953,644 + MMJJ_RS05150 0.33 -2.2 953,679 + MMJJ_RS05150 0.37 -3.3 953,680 - MMJJ_RS05150 0.37 -2.9 953,680 - MMJJ_RS05150 0.37 -1.4 953,680 - MMJJ_RS05150 0.37 -2.0 953,680 - MMJJ_RS05150 0.37 -1.6 953,680 - MMJJ_RS05150 0.37 -2.6 953,681 + MMJJ_RS05150 0.37 -3.2 953,681 + MMJJ_RS05150 0.37 -0.7 953,692 - MMJJ_RS05150 0.38 -2.1 953,736 - MMJJ_RS05150 0.42 -1.1 953,736 - MMJJ_RS05150 0.42 -1.9 953,737 + MMJJ_RS05150 0.42 -2.4 953,822 - MMJJ_RS05150 0.50 -1.8 953,823 + MMJJ_RS05150 0.50 -2.6 953,823 + MMJJ_RS05150 0.50 -2.5 953,871 - MMJJ_RS05150 0.55 -1.9 953,872 + MMJJ_RS05150 0.55 -2.6 953,872 + MMJJ_RS05150 0.55 -1.9 953,901 - MMJJ_RS05150 0.57 -2.2 953,902 + MMJJ_RS05150 0.57 -2.8 953,950 + MMJJ_RS05150 0.62 -1.1 954,029 - MMJJ_RS05150 0.69 -2.5 954,030 + MMJJ_RS05150 0.69 -3.4 954,066 - MMJJ_RS05150 0.73 -2.1 954,088 - MMJJ_RS05150 0.75 -1.4 954,127 + MMJJ_RS05150 0.78 -0.6 954,135 - MMJJ_RS05150 0.79 +0.6 954,238 + MMJJ_RS05150 0.89 -2.3 954,261 - -2.5 954,262 + -2.3 954,262 + -3.2 954,265 - -2.2 954,265 - -0.5 954,266 + -1.5 954,266 + -2.4 954,281 - -1.5 954,287 - -1.8 954,288 + -1.5 954,303 - +0.3 954,304 + -2.7 954,304 + -1.5 954,350 - -2.0 954,351 + -2.2 954,359 + -3.3 954,389 - -3.3 954,390 + -0.8 954,400 - -2.5 954,401 + -2.9 954,401 + -1.0 954,451 - -3.2 954,452 + -2.4 954,456 + -1.1 954,614 - MMJJ_RS05155 0.13 -2.4 954,614 - MMJJ_RS05155 0.13 -3.5 954,614 - MMJJ_RS05155 0.13 -1.6 954,615 + MMJJ_RS05155 0.13 -1.3 954,632 + -0.4 954,812 - MMJJ_RS05155 0.31 -2.9 954,927 - MMJJ_RS05155 0.42 -1.9 955,014 - MMJJ_RS05155 0.50 -2.9 955,015 + MMJJ_RS05155 0.50 -2.8 955,325 - MMJJ_RS05155 0.79 +0.8 955,325 - MMJJ_RS05155 0.79 -1.6 955,325 - MMJJ_RS05155 0.79 -2.6 955,325 - MMJJ_RS05155 0.79 -3.0
Or see this region's nucleotide sequence