Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08140 and MMJJ_RS08145 are separated by 179 nucleotides MMJJ_RS08145 and MMJJ_RS08150 are separated by 62 nucleotides
MMJJ_RS08140: MMJJ_RS08140 - type I glutamate--ammonia ligase, at 1,493,926 to 1,495,266
_RS08140
MMJJ_RS08145: MMJJ_RS08145 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,495,446 to 1,496,735
_RS08145
MMJJ_RS08150: MMJJ_RS08150 - M24 family metallopeptidase, at 1,496,798 to 1,497,817
_RS08150
Position (kb)
1495
1496
1497 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1495.239 kb on + strand at 1495.290 kb on - strand at 1495.322 kb on - strand at 1495.327 kb on + strand at 1495.340 kb on - strand at 1495.340 kb on - strand at 1495.340 kb on - strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.341 kb on + strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.350 kb on - strand at 1495.351 kb on + strand at 1495.351 kb on + strand at 1495.363 kb on + strand at 1495.363 kb on + strand at 1495.363 kb on + strand at 1495.382 kb on + strand at 1495.396 kb on - strand at 1495.403 kb on + strand at 1495.422 kb on + strand at 1495.427 kb on - strand at 1495.454 kb on + strand at 1495.556 kb on - strand at 1495.716 kb on + strand, within MMJJ_RS08145 at 1496.449 kb on - strand at 1496.743 kb on + strand at 1496.755 kb on - strand at 1496.758 kb on - strand at 1496.759 kb on + strand at 1496.761 kb on + strand at 1496.763 kb on - strand at 1496.763 kb on - strand at 1496.771 kb on - strand at 1496.781 kb on + strand at 1496.798 kb on + strand at 1496.830 kb on + strand at 1496.861 kb on + strand at 1496.876 kb on + strand at 1496.877 kb on - strand at 1496.877 kb on - strand at 1496.878 kb on + strand at 1496.878 kb on + strand at 1496.898 kb on + strand at 1496.932 kb on - strand, within MMJJ_RS08150 at 1496.932 kb on - strand at 1496.933 kb on + strand, within MMJJ_RS08150 at 1496.941 kb on - strand, within MMJJ_RS08150 at 1496.942 kb on + strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.953 kb on + strand, within MMJJ_RS08150 at 1496.958 kb on - strand, within MMJJ_RS08150 at 1496.991 kb on + strand, within MMJJ_RS08150 at 1497.037 kb on - strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.111 kb on - strand, within MMJJ_RS08150 at 1497.169 kb on - strand, within MMJJ_RS08150 at 1497.217 kb on - strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.281 kb on + strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.299 kb on + strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.375 kb on - strand, within MMJJ_RS08150 at 1497.378 kb on - strand, within MMJJ_RS08150 at 1497.413 kb on - strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.433 kb on - strand, within MMJJ_RS08150 at 1497.434 kb on + strand, within MMJJ_RS08150 at 1497.436 kb on - strand, within MMJJ_RS08150 at 1497.447 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.680 kb on + strand, within MMJJ_RS08150 at 1497.681 kb on - strand, within MMJJ_RS08150 at 1497.685 kb on + strand, within MMJJ_RS08150 at 1497.722 kb on - strand at 1497.722 kb on - strand at 1497.724 kb on - strand at 1497.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 1,495,239 + +1.8 1,495,290 - +0.1 1,495,322 - +1.2 1,495,327 + +2.0 1,495,340 - -0.0 1,495,340 - +0.5 1,495,340 - +0.7 1,495,341 + -0.9 1,495,341 + -0.4 1,495,341 + -0.3 1,495,341 + +0.3 1,495,350 - -0.1 1,495,350 - -0.4 1,495,350 - +0.7 1,495,350 - -0.4 1,495,351 + +0.1 1,495,351 + -0.2 1,495,363 + +1.7 1,495,363 + +1.4 1,495,363 + +2.3 1,495,382 + +0.2 1,495,396 - -0.4 1,495,403 + +0.6 1,495,422 + +1.8 1,495,427 - +0.6 1,495,454 + +2.2 1,495,556 - +1.5 1,495,716 + MMJJ_RS08145 0.21 +1.3 1,496,449 - +3.0 1,496,743 + +2.9 1,496,755 - -0.1 1,496,758 - -0.1 1,496,759 + -0.1 1,496,761 + +1.0 1,496,763 - -0.6 1,496,763 - +0.2 1,496,771 - -0.8 1,496,781 + +0.3 1,496,798 + -1.4 1,496,830 + -0.3 1,496,861 + -0.8 1,496,876 + -1.4 1,496,877 - +0.3 1,496,877 - -0.6 1,496,878 + -0.5 1,496,878 + -0.7 1,496,898 + -0.8 1,496,932 - MMJJ_RS08150 0.13 +0.4 1,496,932 - +2.0 1,496,933 + MMJJ_RS08150 0.13 -0.7 1,496,941 - MMJJ_RS08150 0.14 +2.5 1,496,942 + MMJJ_RS08150 0.14 +0.3 1,496,952 - MMJJ_RS08150 0.15 -0.4 1,496,952 - MMJJ_RS08150 0.15 -0.3 1,496,953 + MMJJ_RS08150 0.15 -1.8 1,496,958 - MMJJ_RS08150 0.16 -0.5 1,496,991 + MMJJ_RS08150 0.19 -2.4 1,497,037 - MMJJ_RS08150 0.23 +0.6 1,497,038 + MMJJ_RS08150 0.24 -1.6 1,497,038 + MMJJ_RS08150 0.24 -2.2 1,497,111 - MMJJ_RS08150 0.31 -1.1 1,497,169 - MMJJ_RS08150 0.36 -0.8 1,497,217 - MMJJ_RS08150 0.41 -1.6 1,497,218 + MMJJ_RS08150 0.41 +0.4 1,497,218 + MMJJ_RS08150 0.41 -0.3 1,497,218 + MMJJ_RS08150 0.41 -0.3 1,497,281 + MMJJ_RS08150 0.47 +0.6 1,497,298 - MMJJ_RS08150 0.49 -0.0 1,497,298 - MMJJ_RS08150 0.49 +0.2 1,497,299 + MMJJ_RS08150 0.49 +0.3 1,497,301 - MMJJ_RS08150 0.49 -0.7 1,497,301 - MMJJ_RS08150 0.49 -0.5 1,497,375 - MMJJ_RS08150 0.57 -1.1 1,497,378 - MMJJ_RS08150 0.57 +1.7 1,497,413 - MMJJ_RS08150 0.60 +0.3 1,497,414 + MMJJ_RS08150 0.60 +0.1 1,497,414 + MMJJ_RS08150 0.60 -1.5 1,497,433 - MMJJ_RS08150 0.62 +0.3 1,497,434 + MMJJ_RS08150 0.62 +0.5 1,497,436 - MMJJ_RS08150 0.63 -0.6 1,497,447 - MMJJ_RS08150 0.64 -1.0 1,497,577 - MMJJ_RS08150 0.76 -0.3 1,497,577 - MMJJ_RS08150 0.76 -1.7 1,497,578 + MMJJ_RS08150 0.76 +0.2 1,497,578 + MMJJ_RS08150 0.76 -1.0 1,497,604 + MMJJ_RS08150 0.79 -0.9 1,497,604 + MMJJ_RS08150 0.79 -0.9 1,497,680 + MMJJ_RS08150 0.86 +0.3 1,497,681 - MMJJ_RS08150 0.87 -0.7 1,497,685 + MMJJ_RS08150 0.87 +0.2 1,497,722 - -0.6 1,497,722 - -0.8 1,497,724 - -0.4 1,497,724 - -0.3
Or see this region's nucleotide sequence