Experiment: Formate 100 mM hypophosphite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01420 and MMJJ_RS01425 are separated by 65 nucleotides MMJJ_RS01425 and MMJJ_RS01430 are separated by 85 nucleotides MMJJ_RS01430 and MMJJ_RS01435 are separated by 5 nucleotides
MMJJ_RS01420: MMJJ_RS01420 - hypothetical protein, at 277,257 to 278,123
_RS01420
MMJJ_RS01425: MMJJ_RS01425 - cell wall-binding protein, at 278,189 to 279,145
_RS01425
MMJJ_RS01430: MMJJ_RS01430 - hypothetical protein, at 279,231 to 279,776
_RS01430
MMJJ_RS01435: MMJJ_RS01435 - 4Fe-4S binding protein, at 279,782 to 280,483
_RS01435
Position (kb)
278
279
280 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12
13 at 277.232 kb on - strand at 277.233 kb on + strand at 277.233 kb on + strand at 277.233 kb on + strand at 277.234 kb on - strand at 277.234 kb on - strand at 277.235 kb on + strand at 277.240 kb on - strand at 277.240 kb on - strand at 277.240 kb on - strand at 277.241 kb on + strand at 277.253 kb on - strand at 277.254 kb on + strand at 277.264 kb on + strand at 277.330 kb on - strand at 277.331 kb on + strand at 277.365 kb on + strand, within MMJJ_RS01420 at 277.380 kb on - strand, within MMJJ_RS01420 at 277.389 kb on - strand, within MMJJ_RS01420 at 277.389 kb on - strand, within MMJJ_RS01420 at 277.390 kb on + strand, within MMJJ_RS01420 at 277.390 kb on + strand, within MMJJ_RS01420 at 277.397 kb on - strand, within MMJJ_RS01420 at 277.398 kb on + strand, within MMJJ_RS01420 at 277.398 kb on + strand, within MMJJ_RS01420 at 277.399 kb on - strand, within MMJJ_RS01420 at 277.399 kb on - strand, within MMJJ_RS01420 at 277.400 kb on + strand, within MMJJ_RS01420 at 277.400 kb on + strand, within MMJJ_RS01420 at 277.403 kb on - strand, within MMJJ_RS01420 at 277.403 kb on - strand, within MMJJ_RS01420 at 277.445 kb on + strand, within MMJJ_RS01420 at 277.506 kb on - strand, within MMJJ_RS01420 at 277.519 kb on - strand, within MMJJ_RS01420 at 277.534 kb on - strand, within MMJJ_RS01420 at 277.549 kb on - strand, within MMJJ_RS01420 at 277.551 kb on - strand, within MMJJ_RS01420 at 277.551 kb on - strand, within MMJJ_RS01420 at 277.552 kb on + strand, within MMJJ_RS01420 at 277.562 kb on - strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.566 kb on - strand, within MMJJ_RS01420 at 277.586 kb on + strand, within MMJJ_RS01420 at 277.623 kb on + strand, within MMJJ_RS01420 at 277.635 kb on - strand, within MMJJ_RS01420 at 277.825 kb on - strand, within MMJJ_RS01420 at 277.826 kb on + strand, within MMJJ_RS01420 at 277.830 kb on + strand, within MMJJ_RS01420 at 277.860 kb on + strand, within MMJJ_RS01420 at 277.889 kb on + strand, within MMJJ_RS01420 at 277.961 kb on + strand, within MMJJ_RS01420 at 277.961 kb on + strand, within MMJJ_RS01420 at 277.964 kb on - strand, within MMJJ_RS01420 at 277.987 kb on - strand, within MMJJ_RS01420 at 277.987 kb on - strand, within MMJJ_RS01420 at 277.988 kb on + strand, within MMJJ_RS01420 at 277.998 kb on - strand, within MMJJ_RS01420 at 278.029 kb on - strand, within MMJJ_RS01420 at 278.038 kb on + strand at 278.085 kb on - strand at 278.086 kb on + strand at 278.103 kb on + strand at 278.108 kb on - strand at 278.113 kb on + strand at 278.130 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.134 kb on + strand at 278.135 kb on - strand at 278.135 kb on - strand at 278.135 kb on - strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.137 kb on - strand at 278.138 kb on + strand at 278.138 kb on + strand at 278.141 kb on + strand at 278.141 kb on + strand at 278.148 kb on + strand at 278.150 kb on - strand at 278.208 kb on - strand at 278.209 kb on + strand at 278.214 kb on - strand at 278.215 kb on + strand at 278.226 kb on - strand at 278.226 kb on - strand at 278.226 kb on - strand at 278.227 kb on + strand at 278.227 kb on + strand at 278.243 kb on - strand at 278.263 kb on + strand at 278.263 kb on + strand at 278.266 kb on + strand at 278.292 kb on - strand, within MMJJ_RS01425 at 278.302 kb on - strand, within MMJJ_RS01425 at 278.366 kb on + strand, within MMJJ_RS01425 at 278.371 kb on + strand, within MMJJ_RS01425 at 278.379 kb on - strand, within MMJJ_RS01425 at 278.403 kb on - strand, within MMJJ_RS01425 at 278.442 kb on + strand, within MMJJ_RS01425 at 278.468 kb on + strand, within MMJJ_RS01425 at 278.518 kb on - strand, within MMJJ_RS01425 at 278.519 kb on + strand, within MMJJ_RS01425 at 278.519 kb on + strand, within MMJJ_RS01425 at 278.519 kb on + strand, within MMJJ_RS01425 at 278.523 kb on - strand, within MMJJ_RS01425 at 278.524 kb on + strand at 278.527 kb on - strand, within MMJJ_RS01425 at 278.591 kb on + strand, within MMJJ_RS01425 at 278.613 kb on - strand, within MMJJ_RS01425 at 278.623 kb on + strand, within MMJJ_RS01425 at 278.626 kb on + strand, within MMJJ_RS01425 at 278.671 kb on - strand, within MMJJ_RS01425 at 278.686 kb on - strand, within MMJJ_RS01425 at 278.709 kb on + strand, within MMJJ_RS01425 at 278.709 kb on + strand, within MMJJ_RS01425 at 278.715 kb on + strand, within MMJJ_RS01425 at 278.727 kb on - strand, within MMJJ_RS01425 at 278.816 kb on + strand, within MMJJ_RS01425 at 278.841 kb on - strand, within MMJJ_RS01425 at 278.842 kb on + strand, within MMJJ_RS01425 at 278.883 kb on + strand, within MMJJ_RS01425 at 278.883 kb on + strand, within MMJJ_RS01425 at 278.892 kb on - strand, within MMJJ_RS01425 at 278.893 kb on + strand, within MMJJ_RS01425 at 278.903 kb on - strand, within MMJJ_RS01425 at 278.903 kb on - strand, within MMJJ_RS01425 at 278.903 kb on - strand, within MMJJ_RS01425 at 278.903 kb on - strand, within MMJJ_RS01425 at 278.904 kb on + strand, within MMJJ_RS01425 at 278.919 kb on + strand, within MMJJ_RS01425 at 278.924 kb on - strand, within MMJJ_RS01425 at 278.924 kb on - strand, within MMJJ_RS01425 at 278.925 kb on + strand, within MMJJ_RS01425 at 278.925 kb on + strand, within MMJJ_RS01425 at 278.925 kb on + strand, within MMJJ_RS01425 at 278.930 kb on - strand, within MMJJ_RS01425 at 278.962 kb on + strand, within MMJJ_RS01425 at 278.962 kb on + strand, within MMJJ_RS01425 at 278.968 kb on - strand, within MMJJ_RS01425 at 278.969 kb on + strand, within MMJJ_RS01425 at 279.035 kb on + strand, within MMJJ_RS01425 at 279.157 kb on - strand at 279.158 kb on + strand at 279.158 kb on + strand at 279.159 kb on - strand at 279.159 kb on - strand at 279.160 kb on + strand at 279.208 kb on + strand at 279.210 kb on - strand at 279.210 kb on - strand at 279.213 kb on - strand at 279.213 kb on - strand at 279.213 kb on - strand at 279.214 kb on + strand at 279.216 kb on - strand at 279.216 kb on - strand at 279.217 kb on + strand at 279.218 kb on - strand at 279.218 kb on - strand at 279.218 kb on - strand at 279.219 kb on + strand at 279.259 kb on - strand at 279.260 kb on + strand at 279.278 kb on + strand at 279.315 kb on + strand, within MMJJ_RS01430 at 279.354 kb on - strand, within MMJJ_RS01430 at 279.354 kb on - strand at 279.354 kb on - strand, within MMJJ_RS01430 at 279.354 kb on - strand, within MMJJ_RS01430 at 279.355 kb on + strand, within MMJJ_RS01430 at 279.355 kb on + strand, within MMJJ_RS01430 at 279.356 kb on - strand, within MMJJ_RS01430 at 279.363 kb on + strand, within MMJJ_RS01430 at 279.368 kb on - strand, within MMJJ_RS01430 at 279.447 kb on - strand, within MMJJ_RS01430 at 279.448 kb on + strand, within MMJJ_RS01430 at 279.457 kb on - strand, within MMJJ_RS01430 at 279.458 kb on + strand, within MMJJ_RS01430 at 279.458 kb on + strand, within MMJJ_RS01430 at 279.468 kb on - strand, within MMJJ_RS01430 at 279.468 kb on - strand, within MMJJ_RS01430 at 279.469 kb on + strand, within MMJJ_RS01430 at 279.503 kb on - strand, within MMJJ_RS01430 at 279.504 kb on + strand, within MMJJ_RS01430 at 279.567 kb on + strand, within MMJJ_RS01430 at 279.574 kb on - strand, within MMJJ_RS01430 at 279.574 kb on - strand, within MMJJ_RS01430 at 279.588 kb on + strand, within MMJJ_RS01430 at 279.611 kb on + strand, within MMJJ_RS01430 at 279.611 kb on + strand, within MMJJ_RS01430 at 279.635 kb on + strand, within MMJJ_RS01430 at 279.640 kb on + strand, within MMJJ_RS01430 at 279.677 kb on + strand, within MMJJ_RS01430 at 279.681 kb on + strand, within MMJJ_RS01430 at 279.747 kb on + strand at 279.779 kb on - strand at 279.779 kb on - strand at 279.780 kb on + strand at 279.814 kb on + strand at 279.869 kb on + strand, within MMJJ_RS01435 at 279.869 kb on + strand, within MMJJ_RS01435 at 279.873 kb on - strand, within MMJJ_RS01435 at 279.982 kb on - strand, within MMJJ_RS01435 at 279.982 kb on - strand, within MMJJ_RS01435 at 279.983 kb on + strand, within MMJJ_RS01435 at 279.983 kb on + strand, within MMJJ_RS01435 at 279.985 kb on + strand, within MMJJ_RS01435 at 280.016 kb on - strand, within MMJJ_RS01435 at 280.017 kb on + strand, within MMJJ_RS01435 at 280.018 kb on - strand, within MMJJ_RS01435 at 280.019 kb on + strand, within MMJJ_RS01435 at 280.035 kb on - strand, within MMJJ_RS01435 at 280.040 kb on + strand, within MMJJ_RS01435 at 280.040 kb on + strand, within MMJJ_RS01435 at 280.063 kb on + strand, within MMJJ_RS01435 at 280.063 kb on + strand, within MMJJ_RS01435 at 280.091 kb on - strand, within MMJJ_RS01435 at 280.091 kb on - strand, within MMJJ_RS01435 at 280.095 kb on - strand, within MMJJ_RS01435 at 280.100 kb on - strand, within MMJJ_RS01435 at 280.101 kb on + strand at 280.124 kb on - strand, within MMJJ_RS01435 at 280.125 kb on + strand, within MMJJ_RS01435 at 280.125 kb on + strand, within MMJJ_RS01435
Per-strain Table
Position Strand Gene LocusTag Fraction Formate 100 mM hypophosphite remove 277,232 - -1.6 277,233 + +0.4 277,233 + -0.4 277,233 + -0.8 277,234 - +1.7 277,234 - -2.0 277,235 + -0.8 277,240 - +0.4 277,240 - +2.1 277,240 - +0.6 277,241 + -1.0 277,253 - -0.5 277,254 + +0.5 277,264 + +0.7 277,330 - -0.1 277,331 + -0.3 277,365 + MMJJ_RS01420 0.12 +1.1 277,380 - MMJJ_RS01420 0.14 +0.8 277,389 - MMJJ_RS01420 0.15 +1.4 277,389 - MMJJ_RS01420 0.15 -0.9 277,390 + MMJJ_RS01420 0.15 +0.1 277,390 + MMJJ_RS01420 0.15 -0.2 277,397 - MMJJ_RS01420 0.16 -0.2 277,398 + MMJJ_RS01420 0.16 +0.3 277,398 + MMJJ_RS01420 0.16 -0.6 277,399 - MMJJ_RS01420 0.16 +0.4 277,399 - MMJJ_RS01420 0.16 -0.1 277,400 + MMJJ_RS01420 0.16 +1.5 277,400 + MMJJ_RS01420 0.16 +2.6 277,403 - MMJJ_RS01420 0.17 +0.3 277,403 - MMJJ_RS01420 0.17 -2.4 277,445 + MMJJ_RS01420 0.22 -0.6 277,506 - MMJJ_RS01420 0.29 -0.7 277,519 - MMJJ_RS01420 0.30 +0.5 277,534 - MMJJ_RS01420 0.32 +0.8 277,549 - MMJJ_RS01420 0.34 +1.6 277,551 - MMJJ_RS01420 0.34 +0.4 277,551 - MMJJ_RS01420 0.34 +0.4 277,552 + MMJJ_RS01420 0.34 -1.7 277,562 - MMJJ_RS01420 0.35 -0.9 277,563 + MMJJ_RS01420 0.35 +0.0 277,563 + MMJJ_RS01420 0.35 +2.8 277,563 + MMJJ_RS01420 0.35 -0.0 277,566 - MMJJ_RS01420 0.36 +0.4 277,586 + MMJJ_RS01420 0.38 +1.0 277,623 + MMJJ_RS01420 0.42 -0.0 277,635 - MMJJ_RS01420 0.44 +0.4 277,825 - MMJJ_RS01420 0.66 +0.0 277,826 + MMJJ_RS01420 0.66 -0.3 277,830 + MMJJ_RS01420 0.66 -0.2 277,860 + MMJJ_RS01420 0.70 -0.9 277,889 + MMJJ_RS01420 0.73 +4.6 277,961 + MMJJ_RS01420 0.81 -1.0 277,961 + MMJJ_RS01420 0.81 -1.2 277,964 - MMJJ_RS01420 0.82 -2.1 277,987 - MMJJ_RS01420 0.84 -0.0 277,987 - MMJJ_RS01420 0.84 -0.4 277,988 + MMJJ_RS01420 0.84 +0.6 277,998 - MMJJ_RS01420 0.85 +1.1 278,029 - MMJJ_RS01420 0.89 +4.1 278,038 + +0.4 278,085 - -0.0 278,086 + +0.3 278,103 + +0.5 278,108 - +0.4 278,113 + +1.4 278,130 - -0.6 278,133 - +0.6 278,133 - -1.0 278,133 - -0.8 278,133 - +0.7 278,134 + -0.4 278,135 - -1.1 278,135 - -0.4 278,135 - +2.1 278,136 + +0.1 278,136 + -0.0 278,136 + -1.3 278,136 + +1.1 278,136 + -1.1 278,137 - +0.3 278,138 + -0.4 278,138 + +0.3 278,141 + +0.3 278,141 + -0.5 278,148 + -1.4 278,150 - -0.0 278,208 - +3.0 278,209 + +0.0 278,214 - -0.7 278,215 + -0.5 278,226 - -0.3 278,226 - +0.1 278,226 - -0.2 278,227 + -0.9 278,227 + -1.3 278,243 - -0.8 278,263 + -1.8 278,263 + +0.4 278,266 + -0.7 278,292 - MMJJ_RS01425 0.11 +0.3 278,302 - MMJJ_RS01425 0.12 +1.1 278,366 + MMJJ_RS01425 0.18 +0.7 278,371 + MMJJ_RS01425 0.19 +0.3 278,379 - MMJJ_RS01425 0.20 +0.6 278,403 - MMJJ_RS01425 0.22 +1.0 278,442 + MMJJ_RS01425 0.26 -0.9 278,468 + MMJJ_RS01425 0.29 +2.0 278,518 - MMJJ_RS01425 0.34 -0.8 278,519 + MMJJ_RS01425 0.34 +0.4 278,519 + MMJJ_RS01425 0.34 -1.2 278,519 + MMJJ_RS01425 0.34 -1.4 278,523 - MMJJ_RS01425 0.35 +0.6 278,524 + +2.3 278,527 - MMJJ_RS01425 0.35 +0.2 278,591 + MMJJ_RS01425 0.42 -0.4 278,613 - MMJJ_RS01425 0.44 -0.3 278,623 + MMJJ_RS01425 0.45 -1.7 278,626 + MMJJ_RS01425 0.46 +2.1 278,671 - MMJJ_RS01425 0.50 -0.5 278,686 - MMJJ_RS01425 0.52 +0.3 278,709 + MMJJ_RS01425 0.54 -1.8 278,709 + MMJJ_RS01425 0.54 +0.9 278,715 + MMJJ_RS01425 0.55 -0.3 278,727 - MMJJ_RS01425 0.56 -0.5 278,816 + MMJJ_RS01425 0.66 +0.3 278,841 - MMJJ_RS01425 0.68 +2.4 278,842 + MMJJ_RS01425 0.68 +0.7 278,883 + MMJJ_RS01425 0.73 +0.4 278,883 + MMJJ_RS01425 0.73 +0.8 278,892 - MMJJ_RS01425 0.73 -0.4 278,893 + MMJJ_RS01425 0.74 +0.9 278,903 - MMJJ_RS01425 0.75 +0.0 278,903 - MMJJ_RS01425 0.75 +0.1 278,903 - MMJJ_RS01425 0.75 -0.3 278,903 - MMJJ_RS01425 0.75 -0.7 278,904 + MMJJ_RS01425 0.75 +13.7 278,919 + MMJJ_RS01425 0.76 -1.5 278,924 - MMJJ_RS01425 0.77 +0.4 278,924 - MMJJ_RS01425 0.77 -1.1 278,925 + MMJJ_RS01425 0.77 +0.3 278,925 + MMJJ_RS01425 0.77 -0.7 278,925 + MMJJ_RS01425 0.77 -1.2 278,930 - MMJJ_RS01425 0.77 -1.4 278,962 + MMJJ_RS01425 0.81 +0.0 278,962 + MMJJ_RS01425 0.81 -1.8 278,968 - MMJJ_RS01425 0.81 +2.1 278,969 + MMJJ_RS01425 0.82 +0.2 279,035 + MMJJ_RS01425 0.88 -0.1 279,157 - +1.2 279,158 + +0.2 279,158 + -1.0 279,159 - +1.0 279,159 - -0.8 279,160 + -0.6 279,208 + +0.1 279,210 - +1.1 279,210 - -0.6 279,213 - +0.3 279,213 - +0.2 279,213 - +2.4 279,214 + +0.1 279,216 - +0.4 279,216 - +0.0 279,217 + -0.0 279,218 - -2.2 279,218 - +0.3 279,218 - +0.2 279,219 + +0.5 279,259 - -0.7 279,260 + -0.1 279,278 + +1.0 279,315 + MMJJ_RS01430 0.15 -0.7 279,354 - MMJJ_RS01430 0.23 -1.4 279,354 - +2.4 279,354 - MMJJ_RS01430 0.23 -1.1 279,354 - MMJJ_RS01430 0.23 +0.2 279,355 + MMJJ_RS01430 0.23 +0.2 279,355 + MMJJ_RS01430 0.23 -0.3 279,356 - MMJJ_RS01430 0.23 -0.1 279,363 + MMJJ_RS01430 0.24 +0.2 279,368 - MMJJ_RS01430 0.25 +0.0 279,447 - MMJJ_RS01430 0.40 -0.4 279,448 + MMJJ_RS01430 0.40 -0.5 279,457 - MMJJ_RS01430 0.41 -0.6 279,458 + MMJJ_RS01430 0.42 -0.8 279,458 + MMJJ_RS01430 0.42 -0.8 279,468 - MMJJ_RS01430 0.43 +0.1 279,468 - MMJJ_RS01430 0.43 -1.3 279,469 + MMJJ_RS01430 0.44 -0.8 279,503 - MMJJ_RS01430 0.50 +0.2 279,504 + MMJJ_RS01430 0.50 -0.2 279,567 + MMJJ_RS01430 0.62 -0.3 279,574 - MMJJ_RS01430 0.63 -0.7 279,574 - MMJJ_RS01430 0.63 +2.1 279,588 + MMJJ_RS01430 0.65 +0.9 279,611 + MMJJ_RS01430 0.70 +0.1 279,611 + MMJJ_RS01430 0.70 +0.9 279,635 + MMJJ_RS01430 0.74 -0.2 279,640 + MMJJ_RS01430 0.75 +3.0 279,677 + MMJJ_RS01430 0.82 +2.5 279,681 + MMJJ_RS01430 0.82 -0.1 279,747 + -1.2 279,779 - +0.5 279,779 - +0.1 279,780 + +0.4 279,814 + -0.6 279,869 + MMJJ_RS01435 0.12 +0.3 279,869 + MMJJ_RS01435 0.12 -0.6 279,873 - MMJJ_RS01435 0.13 -1.1 279,982 - MMJJ_RS01435 0.28 +1.4 279,982 - MMJJ_RS01435 0.28 +0.4 279,983 + MMJJ_RS01435 0.29 -3.0 279,983 + MMJJ_RS01435 0.29 -0.5 279,985 + MMJJ_RS01435 0.29 +2.4 280,016 - MMJJ_RS01435 0.33 -0.2 280,017 + MMJJ_RS01435 0.33 -0.0 280,018 - MMJJ_RS01435 0.34 +0.2 280,019 + MMJJ_RS01435 0.34 +1.0 280,035 - MMJJ_RS01435 0.36 +0.5 280,040 + MMJJ_RS01435 0.37 -0.1 280,040 + MMJJ_RS01435 0.37 -0.5 280,063 + MMJJ_RS01435 0.40 -1.2 280,063 + MMJJ_RS01435 0.40 +1.2 280,091 - MMJJ_RS01435 0.44 -1.0 280,091 - MMJJ_RS01435 0.44 +0.0 280,095 - MMJJ_RS01435 0.45 +1.4 280,100 - MMJJ_RS01435 0.45 +0.2 280,101 + +3.6 280,124 - MMJJ_RS01435 0.49 -0.3 280,125 + MMJJ_RS01435 0.49 -0.6 280,125 + MMJJ_RS01435 0.49 -1.9
Or see this region's nucleotide sequence